Occurrence of Extended Spectrum Beta Lactamase Encoding Genes among Urinary Pathogenic <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates Obtained from a Tertiary Hospital in Gombe Nigeria

Abstract

This study was conducted to assess the occurrence and nature of extended-spectrum beta lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae isolates from patients who presented with urinary tract infection at Federal Teaching Hospital Gombe. Isolates collected were recovered on MacConkey agar at 35˚C and were identified as members of Enterobacteriaceae, and further screened for antimicrobial susceptibility and resistance by disc diffusion method. Isolates resistant to oxyimino-cephalosporins were confirmed as ESBL producers using Double Disks Synergy Test (DDST). The study shows 66% resistance to ceftriaxone (30 µg) in K. pneumoniae, which was the highest value recorded and a 51% resistance to cefpodoxime (10 µg) in E. coli. The sensitivity of E. coli and K. pneumoniae isolates to cefpodoxime (10 µg) were 49% and 33.9% respectively. ESBLs were detected among 40% (40/100) of E. coli and 54.13% (59/109) of K. pneumoniae isolates. Molecular characterization of ESBL encoding genes among E. coli isolates using multiplex-PCR showed 10% prevalence of SHV gene and 5% prevalence for CTX-M gene while TEM gene was not detected. In K. pneumoniae isolates, 5% prevalence was recorded for each of the three genes screened. The study revealed a co-occurrence of SHV and CTX-M in 75% of the E. coli and 70% of the K. pneumoniae isolates; the occurrence of all the three genes was seen in 10% and 5% of K.

Antibiotic resistance and virulence profiles of Proteus mirabilis isolated from broiler chickens at abattoir in South Africa

“…Beta‐lactam antibiotics are a class of broad‐spectrum antibiotics consisting of all antibiotic agents that contain a beta‐lactam ring in their molecular structures and are frequently prescribed antimicrobial agents all over the world in the treatment of infections caused by Gram‐positive and Gram‐negative bacteria (Bradford, 2001 ; Yarima et al., 2020 ). Pathogenic Gram‐negative bacteria frequently produce Extended‐Spectrum Beta‐Lactamases (ESBLs), a genetic enzymatic component that contributes to their resistance to beta‐lactam antimicrobial medicines (Pitout et al., 2005 ; Rao et al., 2014 ).…”