Assessment of dioxin-like activity in ambient air particulate matter using recombinant yeast assays

Olivares, Alba; Drooge, Barend L. van; Ballesta, Pascual Pérez; Grimalt, Joan O.; Piña, Benjamı́n

doi:10.1016/j.atmosenv.2010.09.039

Cited by 17 publications

(16 citation statements)

References 25 publications

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“…The biological response correlated better with several high molecular weight PAHs. This correlation can be compared with the excellent correlation between calculated TEQ values and BAPeq observed for the yeast-based YCM-RYA assay (Table 2), in agreement with previous results with air particle samples (Olivares et al, 2011). Whereas a higher dispersion of results form the zebrafish assay may be considered a consequence of the higher complexity of the system, we think that this unexplained high activity of DT2 may be also linked to their deleterious effects on the embryo development (Fig.…”

Section: Correlation Between Biological Activity Of Samples and Theirsupporting

confidence: 91%

“…Table 1 lists AhR-ligand activities for the six measured extracts, as calculated from the plots in Fig. 2, and compares them with those obtained with the yeast AhR-RYA assay (Olivares et al, 2011). Activity values, expressed as BAPeq, varied by more than one order of magnitude in both assays.…”

Section: Assessment Of Ahr-binding Activity In Pah-rich Samples By Thmentioning

confidence: 99%

“…They were obtained on 02e03/10/ 2008 (A1), 21e22/10/2008 (A2), 17e18/11/2008 (A3) and 27e28/11/2008 (A4)) and provide a gradient of PAH concentration related to biomass combustion (Van Drooge and Perez Ballesta, 2009) as well as a range of dioxin-like activity determined by AhR-RYA (Olivares et al, 2011). Coal tar condensates were sampled from spontaneous combustions in two coal waste gobs in the Datong district (Shanxi Province, China) (Querol et al, 2011).…”

Section: Field Samplesmentioning

confidence: 99%

“…Collection and extraction of samples for chemical and biological analyses were performed as described elsewhere (Olivares et al, 2011;Querol et al, 2008;van Drooge et al, 2010). All samples were extracted with a mixture of dichloromethane and methanol (2:1 v/v) for the assays.…”

Section: Sample Treatmentmentioning

confidence: 99%

“…Therefore, assays to identify activation and subsequent signal transduction of AhR are useful for pollution load monitoring in environmental samples (Fent, 2003). A variety of aryl hydrocarbon-responsive reporter assays based on mammalian cell lines (Garrison et al, 1996;Hamers et al, 2000;Schoeters et al, 2004) or genetically modified yeast strains (Misaki et al, 2007;Murahashi et al, 2007;Noguerol et al, 2006b;Olivares et al, 2011) have become common tools to detect AhR-binding activities in different samples and matrices. Bioassays do not provide a chemical identification of the AhR ligands but their low cost and easiness of handling makes them a first choice when testing large numbers of samples or compounds.…”

Section: Introductionmentioning

confidence: 99%

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Developmental effects of aerosols and coal burning particles in zebrafish embryos

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Section: Correlation Between Biological Activity Of Samples and Theirsupporting

confidence: 91%

Section: Assessment Of Ahr-binding Activity In Pah-rich Samples By Thmentioning

confidence: 99%

Section: Field Samplesmentioning

confidence: 99%

Section: Sample Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Developmental effects of aerosols and coal burning particles in zebrafish embryos

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et al. 2013

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Toxicity of atmospheric particle-bound PAHs: an environmental perspective

Mesquita

Drooge

Barata

et al. 2014

Environ Sci Pollut Res

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Atmospheric polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants that represent a risk not only to humans, but to all living organisms. High-molecular weight PAHs are more toxic than lighter relatives, and also have a higher tendency to bind onto air particles (i.e., particle matter, PM). PM is a major constituent of air pollution. Adequate assessment of the biological impact of PM requires the analysis, not only of the effects on human health, but also on the environment. Since the aquatic systems work as a natural sink to these air pollutants, assessing the effects of particle-bound PAHs on aquatic organisms may further characterize its potential aquatic toxicity, also providing simple and low-cost alternative assays to investigate PM biological effects in vivo. We review the current scientific literature, addressing the atmospheric PAHs fate, transformation and deposition, pertinent particle-bound PAHs toxicity data, and the potential aquatic toxic burden. Conceptual and experimental procedures that could improve future investigations and risk assessments are also considered.

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Toxic potential of organic constituents of submicron particulate matter (PM1) in an urban road site (Barcelona)

Mesquita

Drooge

Dall’Osto

et al. 2017

Environ Sci Pollut Res

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Atmospheric particulate matter (PM) is a recognized risk factor contributing to a number of diseases in human populations and wildlife globally. Organic matter is a major component of PM, but its contribution to overall toxicity of PM has not been thoroughly evaluated yet. In the present work, the biological activity of organic extracts from PM1 (particles with less than 1 μm of aerodynamic diameter) collected from an urban road site in the centre of Barcelona (NE Spain) was evaluated using a yeast-based assay (AhR-RYA) and different gene expression markers in zebrafish embryos. Dioxin-like activity of the extracts correlated to primary emissions from local traffic exhausts, reflecting weekday/weekend alternance. Expression levels of cyp1a and of gene markers for key cellular processes and development (ier2, fos) also correlated to vehicle emissions, whereas expression of gene markers related to antioxidant defence and endocrine effects (gstal, hao1, ttr) was strongly reduced in samples with strong contribution from regional air masses with aged secondary organic species or with strong influence of biomass burning emissions. Our data suggest that the toxic potential of PM1 organic chemical constituents strongly depends on the emission sources and on the process of ageing from primary to secondary organic aerosols.

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Assessment of dioxin-like activity in ambient air particulate matter using recombinant yeast assays

Cited by 17 publications

References 25 publications

Developmental effects of aerosols and coal burning particles in zebrafish embryos

Developmental effects of aerosols and coal burning particles in zebrafish embryos

Toxicity of atmospheric particle-bound PAHs: an environmental perspective

Toxic potential of organic constituents of submicron particulate matter (PM1) in an urban road site (Barcelona)

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