Developmental effects of aerosols and coal burning particles in zebrafish embryos

Olivares, Alba; Drooge, Barend L. van; Casado, Marta; Prats, Eva; Serra, Montserrat; Ven, Leo T. van der; Kamstra, Jorke H.; Hamers, Timo; Hermsen, Sanne A.B.; Grimalt, Joan O.; Piña, Benjamı́n

doi:10.1016/j.envpol.2013.02.026

Cited by 20 publications

(6 citation statements)

References 63 publications

(82 reference statements)

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“…Oxy-PAHs have also been linked to developmental toxicity in zebrafish (Wincent et al, 2015). Furthermore, fine PMinduced increases in metabolizing enzymes (Olivares et al, 2013) and developmental effects (Mesquita et al, 2016) in zebrafish strongly correlated with PAH content. By contrast, the relative inactivity of F1 was likely due to a predominance of inert nonpolar unburned fuel components (ie, alkanes and alkenes).…”

Section: Discussionmentioning

confidence: 99%

Zebrafish Locomotor Responses Reveal Irritant Effects of Fine Particulate Matter Extracts and a Role for TRPA1

Stevens

Padilla²,

DeMarini³

et al. 2017

Toxicological Sciences

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Exposure to fine particulate matter (PM) air pollution causes adverse cardiopulmonary outcomes. Yet, the limited capacity to readily identify contributing PM sources and associated PM constituents in any given ambient air shed impedes risk assessment efforts. The health effects of PM have been attributed in part to its capacity to elicit irritant responses. A variety of chemicals trigger irritant behavior responses in zebrafish that can be easily measured. The purposes of this study were to examine the utility of zebrafish locomotor responses in the toxicity assessment of fine PM and its chemical fractions and uncover mechanisms of action. Locomotor responses were recorded in 6-day-old zebrafish exposed for 60 min in the dark at 26 °C to the extractable organic matter of a compressor-generated diesel exhaust PM (C-DEP) and 4 of its fractions (F1-F4) containing varying chemical classes of increasing polarity. The role of the transient receptor potential (TRP) cation channel TRPA1, a chemical sensor in mammals and zebrafish, in locomotor responses to C-DEP, was also examined. Acrolein, an environmental irritant and known activator of TRPA1, and all extracts induced concentration-dependent locomotor responses whose potencies ranked as follows: polar F3 > weakly polar F2 > C-DEP > highly polar F4 > nonpolar F1, indicating that polar and weakly polar fractions that included nitro- and oxy-polyaromatic hydrocarbons (PAHs), drove C-DEP responses. Irritant potencies in fish positively correlated with mutagenic potencies of the same extracts in strains of Salmonella sensitive to nitro- and oxy-PAHs, further implicating these chemical classes in the zebrafish responses to C-DEP. Pharmacologic inhibition of TRPA1 blocked locomotor responses to acrolein and the extracts. Taken together, these data indicate that the zebrafish locomotor assay may help expedite toxicity screening of fine PM sources, identify causal chemical classes, and uncover plausible biological mechanisms.

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Section: Discussionmentioning

confidence: 99%

Zebrafish Locomotor Responses Reveal Irritant Effects of Fine Particulate Matter Extracts and a Role for TRPA1

Stevens

Padilla²,

DeMarini³

et al. 2017

Toxicological Sciences

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“…Zebrafish model is used for determining toxicity associated to air pollution [7] , [8] , [9] , the toxicodynamic mechanisms of the pollutants found in the air used to be conserved through evolution. Althought zebrafish model has been intensively used for determining developmental toxicity associated to air pollutants [7] , [8] , [9] , information on the effects of these pollutants on the sleep/wake cycles in zebrafish is, however, still scarce, probably because of the difficulty for the environmental toxicology labs to find medium- and high-throughput protocols suitable for assessing this endpoint.…”

Section: Description Of Protocolmentioning

confidence: 99%

Analysis of sleep/wake cycles in zebrafish larvae

Ricarte,

Prats,

Bedrossiantz

et al. 2024

MethodsX

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“…In general, it is recognized that neither the amount of PM emission nor chemical analysis alone provides sufficient information to estimate the overall toxicity of combustion-derived particles [7,18,29,51]. Accordingly, the use of effect-based methods (e.g., in vitro tests) is recommended in order to allow a more complete view on the toxic effects combustion particle may exert [7,22,29,51,92].…”

Section: Studies' Conclusion On the Potential Of Combustion-derived Pmentioning

confidence: 99%

From the air to the water phase: implication for toxicity testing of combustion-derived particles

Schmidt

Altenburger

Kühnel

2017

Biomass Conv. Bioref.

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The biological effects of airborne particulate matter (PM) in humans stimulated many research activities in recent years. One type of particles contributing to PM are those derived from combustion processes. By using in vitro test systems for assessing the toxic effects of those particles, the transfer into liquid media is necessary. Thus the question arises, how the transfer of airborne particles into liquid suspensions should be realized and how the exposure of test organisms/cells to the particles can be done and monitored with regard to concentration and stability. Further, the characterization of the airborne particles regarding their composition seems to be of importance in order to understand the induced biological effects. Hence, this review gathers and evaluates results from in vitro and ecotoxicity studies, where the introduction of airborne particles into liquids is required. The aim was to learn on the current practice and recommend on the design of toxicity studies in the future. In total, about 80 papers were retrieved by literature search that deal with the assessment of biological effects of combustion-derived particles or their constituents. The papers were evaluated in detail with regard to the methodology applied. From this evaluation and based on expert knowledge regarding nanoparticle testing, recommendations for the assessment of PM applying toxicological assays were developed. To provide guidance, essential criteria are developed that should help in preparation, testing, and during the evaluation phase of toxicological assays. We further advise whole particle testing, complemented by testing of particle extracts and washed particles.

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Developmental effects of aerosols and coal burning particles in zebrafish embryos

Cited by 20 publications

References 63 publications

Zebrafish Locomotor Responses Reveal Irritant Effects of Fine Particulate Matter Extracts and a Role for TRPA1

Zebrafish Locomotor Responses Reveal Irritant Effects of Fine Particulate Matter Extracts and a Role for TRPA1

Analysis of sleep/wake cycles in zebrafish larvae

From the air to the water phase: implication for toxicity testing of combustion-derived particles

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