Assessment of bacterial populations associated with banana tree roots and development of successful plant probiotics for banana crop

Marcano, Iris-Esther; Díaz-Alcántara, César-Antonio; Urbano, Beatriz; González‐Andrés, Fernando

doi:10.1016/j.soilbio.2016.04.013

Cited by 28 publications

(21 citation statements)

References 72 publications

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“…The use of plant probiotic microorganisms has been shown to hold promise for improving plant health, nutrition, and stress resilience [ 18 , 23 – 25 ], and the delivery of such plant probiotics via for instance bioorganic fertilizers has proven particularly effective in improving soil microbial functionality [ 26 – 29 ]. Although potentially effective, the mechanisms driving the success of such bioorganic fertilizer applications are generally not well described.…”

Section: Introductionmentioning

confidence: 99%

Bio-organic fertilizers stimulate indigenous soil Pseudomonas populations to enhance plant disease suppression

et al. 2020

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Background Plant diseases caused by fungal pathogen result in a substantial economic impact on the global food and fruit industry. Application of organic fertilizers supplemented with biocontrol microorganisms (i.e. bioorganic fertilizers) has been shown to improve resistance against plant pathogens at least in part due to impacts on the structure and function of the resident soil microbiome. However, it remains unclear whether such improvements are driven by the specific action of microbial inoculants, microbial populations naturally resident to the organic fertilizer or the physical-chemical properties of the compost substrate. The aim of this study was to seek the ecological mechanisms involved in the disease suppressive activity of bio-organic fertilizers. Results To disentangle the mechanism of bio-organic fertilizer action, we conducted an experiment tracking Fusarium wilt disease of banana and changes in soil microbial communities over three growth seasons in response to the following four treatments: bio-organic fertilizer (containing Bacillus amyloliquefaciens W19), organic fertilizer, sterilized organic fertilizer and sterilized organic fertilizer supplemented with B. amyloliquefaciens W19. We found that sterilized bioorganic fertilizer to which Bacillus was re-inoculated provided a similar degree of disease suppression as the non-sterilized bioorganic fertilizer across cropping seasons. We further observed that disease suppression in these treatments is linked to impacts on the resident soil microbial communities, specifically by leading to increases in specific Pseudomonas spp.. Observed correlations between Bacillus amendment and indigenous Pseudomonas spp. that might underlie pathogen suppression were further studied in laboratory and pot experiments. These studies revealed that specific bacterial taxa synergistically increase biofilm formation and likely acted as a plant-beneficial consortium against the pathogen. Conclusion Together we demonstrate that the action of bioorganic fertilizer is a product of the biocontrol inoculum within the organic amendment and its impact on the resident soil microbiome. This knowledge should help in the design of more efficient biofertilizers designed to promote soil function.

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Section: Introductionmentioning

confidence: 99%

Bio-organic fertilizers stimulate indigenous soil Pseudomonas populations to enhance plant disease suppression

et al. 2020

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“…The use of plant probiotic microorganisms has been shown to hold promise for improving plant health, nutrition, and stress resilience [18,[23][24][25], and the delivery of such plant probiotics via for instance bioorganic fertilizers has proven particularly effective in improving soil microbial functionality [26][27][28][29].…”

mentioning

confidence: 99%

Bio-organic fertilizers stimulate indigenous soil Pseudomonas populations to enhance plant disease suppression

Tao

Xiong

et al. 2020

Preprint

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Background: Plant diseases caused by fungal pathogen result in a substantial economic impact on the global food and fruit industry. Application of organic fertilizers supplemented with biocontrol microorganisms (i.e. bioorganic fertilizers) has been shown to improve resistance against plant pathogens at least in part due to impacts on the structure and function of the resident soil microbiome. However, it remains unclear whether such improvements are driven by the specific action of microbial inoculants, microbial populations naturally resident to the organic fertilizer or the physical-chemical properties of the compost substrate. The aim of this study was to seek the ecological mechanisms involved in the disease suppressive activity of bio-organic fertilizers.Results: To disentangle the mechanism of bio-organic fertilizer action, we conducted an experiment tracking Fusarium wilt disease of banana and changes in soil microbial communities over three growth seasons in response to the following four treatments: bio-organic fertilizer (containing Bacillus amyloliquefaciens W19), organic fertilizer, sterilized organic fertilizer and sterilized organic fertilizer supplemented with B. amyloliquefaciens W19. We found that sterilized bioorganic fertilizer to which Bacillus was re-inoculated provided a similar degree of disease suppression as the non-sterilized bioorganic fertilizer. We further observed that disease suppression in these treatments could be linked to impacts on the resident soil microbial communities, with noted increases in specific Pseudomonas spp.. The link between Bacillus amendment and indigenous Pseudomonas spp. was further examined using pot experiments and biofilm assays. Conclusion: Together we demonstrate that the action of bioorganic fertilizer is a product of the biocontrol inoculum within the organic amendment and its impact on the resident soil microbiome. This knowledge should help in the design of more efficient biofertilizers designed to promote soil function.

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“…Different authors have reported the incidence of those genera and species associated to banana crop in several parts in the world, such as India (Karthik et al, 2017;Rajamanickam et al, 2018), the Dominican Republic (Marcano et al, 2016), Kenya (Ngamau et al, 2012) and Brazil (Souza et al, 2013). The frequency of these endophytes may be associated with the conditions of the environment and the management of the banana crop.…”

Section: Resultsmentioning

confidence: 99%

Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)

et al. 2018

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The objective of this study was to characterize banana tree endophytic bacteria at genus and species level and to determine the metabolic reactions associated with the nitrogen transformations. The identification at genus and species levels was performed using the partial sequencing of the rDNA 16S region. The assimbyotic nitrogen fixation, the reduction of nitrate and the production of urease were in vitro evaluated. The DNA of the bacterial isolates was also amplified to verify the presence of the nifH, nirK and nirS regions. Biochemical tests were performed in a complete randomized design; the treatments consisted of 39 bacterial isolates with three replications. Sequence analysis enabled the identification of four genera: Bacillus, Rhizobium, Klebsiella and Enterobacter. The Bacillus genus occurred more frequently, nine species were identified. By evaluating the results of biochemical tests, it was observed that three isolates showed multiple abilities: growth in NFb medium, nitrate reduction and production of urease. The isolates belong to the genus Bacillus and of the species subtilis, thuringienses and amyloliquefaciens. Approximately 12.5% of the isolates amplified the region corresponding to the nifH gene, 7.5% amplified gene nirK and 3.9% amplified the nirS gene. Endophytic bacteria evaluated in the present study showed in vitro activity for urease, nitrate reductase enzymes, however, relevant nitrogenase activity was not observed.

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Assessment of bacterial populations associated with banana tree roots and development of successful plant probiotics for banana crop

Cited by 28 publications

References 72 publications

Bio-organic fertilizers stimulate indigenous soil Pseudomonas populations to enhance plant disease suppression

Bio-organic fertilizers stimulate indigenous soil Pseudomonas populations to enhance plant disease suppression

Bio-organic fertilizers stimulate indigenous soil Pseudomonas populations to enhance plant disease suppression

Characterization and activity of endophytic bacteria from ‘Prata Anã’ banana crop (Musa sp., AAB)

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