Assessment of Antioxidant and Phenolic Compound Concentrations as well as Xanthine Oxidase and Tyrosinase Inhibitory Properties of Different Extracts ofPleurotus citrinopileatusFruiting Bodies

Abstract

Cellular damage caused by reactive oxygen species has been implicated in several diseases, thus establishing a significant role for antioxidants in maintaining human health. Acetone, methanol, and hot water extracts of Pleurotus citrinopileatus were evaluated for their antioxidant activities against β-carotene-linoleic acid and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power, ferrous ion-chelating abilities, and xanthine oxidase inhibitory activities. In addition, the tyrosinase inhibitory effects and phenolic compound contents of the extracts were also analyzed. Methanol and acetone extracts of P. citrinopileatus showed stronger inhibition of β-carotene-linoleic acid compared to the hot water extract. Methanol extract (8 mg/mL) showed a significantly high reducing power of 2.92 compared to the other extracts. The hot water extract was more effective than the acetone and methanole extracts for scavenging DPPH radicals. The strongest chelating effect (92.72%) was obtained with 1.0 mg/mL of acetone extract. High performance liquid chromatography analysis detected eight phenolic compounds, including gallic acid, protocatechuic acid, chlorogenic acid, ferulic acid, naringenin, hesperetin, formononetin, and biochanin-A, in an acetonitrile and hydrochloric acid (5 : 1) solvent extract. Xanthine oxidase and tyrosinase inhibitory activities of the acetone, methanol, and hot water extracts increased with increasing concentration. This study suggests that fruiting bodies of P. citrinopileatus can potentially be used as a readily accessible source of natural antioxidants.

Comparative biological activities of extracts from three Malaysian Beilschmiedia species

“…Gallic acid, (-)-epicatechin and procyanidin B2 are examples of phenolic acids and flavonoids that are known as tyrosinase inhibitors. The formation of hydrogen bonding of the available hydroxyl groups of these compounds with the active site of the enzyme is suggested as the key factor for tyrosinase inhibition (28)(29)(30). It is worth mentioning that, the extracts of B. pulverulenta had the highest TPC and significant radical scavenger, which is consistent with a previous study which found a relationship between the anti-inflammatory activity and the presence of polyphenols (31).…”

Selenium and Zinc Biofortification of Pleurotus eryngii Mycelium and Fruiting Bodies as a Tool for Controlling Their Biological Activity

“…We determined seven phenolic compounds with phenylalanine as a dominating agent in fruiting bodies and mycelium of P. eryngii. In turn, 12 phenolic acids, namely gallic, protocatechuic, chlorogenic, caffeic, vanillin, ferulic, naringin, resveratrol, naringenin, hesperetin, formononetin, and biochanin-A were detected in fruiting bodies of P. citrinopileatus [18]. Gallic acid, protocatechuic acid, chlorogenic acid, vanillin, ferulic acid, naringin, naringenin, hesperetin, formononetin, and biochanin-A were detected from acetonitrile and 0.1 N hydrochloric acid solvent extract of P. eryngii fruiting bodies, and protocatechuic acid was determined in the highest concentration [35].…”

“…Sporocarp contains almost 20% of protein in dry matter and up to 4% fat, which makes it more nutritious when compared to most of the cultivated mushrooms [3,4]. Generally, mushroom species contain from 3 to 15 phenolic compounds, and gallic, and protocatechuic acids are the most commonly reported [18]. All pohenolics are used as indicators of the antioxidant capacity, which is linked to their hydroxyl groups [19].…”

Xanthine Oxidase Inhibitory Activity of Hungarian Wild-Growing Mushrooms

“…Although sporadic investigations have occurred over the last decade, to our knowledge, this is the first study to screen large number of mushroom species for their potential to inhibit XO activity. Previous studies revealed the XO inhibitory activity of some species, Fistulina hepatica , Lentinus lepideus , Phellinus linteus , and Pleurotus cornucopiae , among others (Song et al ., ; Ribeiro et al ., ; Alam et al ., ; Yoon et al ., ). Armillaria mellea and Pleurotus eryngii , investigated in the present study, have been previously studied for their XO‐inhibiting activity.…”