Assessment of Antioxidant and Cytotoxic Activities and Identification of Phenolic Compounds of Centaurea solstitialis and Urospermum picroides from Turkey

Abstract

It is known that some genera of the Asteraceae family are commonly used in Turkish folk medicine. Several studies have investigated the biological effects of different extracts of Centaurea and Urospermum species, but studies involving the phenolic composition of C. solstitialis and U. picroides extracts are very limited. This study aimed to investigate the phenolic composition and antioxidant activity of C. solstitialis and U. picroides and evaluate their possible cytotoxic effect. RP-HPLC analysis was used to elucidate the phenolic profiles of the ethanolic extracts of flowering parts of C. solstitialis and U. picroides.The both ethanolic extracts were assessed for their antioxidant properties using DPPH, FRAP, phosphomolybdenum and metal chelating assays. Furthermore, the effect of the extracts on cell viability was evaluated against MCF-7 and PC-3 cancer cells and HEK293 cell line using the MTT assay. The most abundant phenolic compound in both extracts was determined to be caffeic acid, and the amount of this compound was 24078.03 HIGHLIGHTS  The phenolic composition, antioxidant activity and cytotoxic potential of the extracts of C. solstitialis and U. picroides were investigated. Caffeic acid was found as the most abundant phenolic compound in the extracts. Both species showed promising antioxidant activity towards different assays. The highest cytotoxic potential was observed in the extract of C. solstitialis.

Antioxidant Properties, Total Phenolic Content and LC-MS/MS Analysis of Mentha Pulegium, Lepidium Draba and Centaurea Solstitialis

“…In the study by Alper et al, (2021) phenolic components in the ethanolic extract of Centaurea solstitialis gallic acid, 4-hydroxybenzoic acid, chlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid and naringin were found as 92.59, 2 077.33, 52.16, 24 078.00, 41.00, 127.21 and 120.62 μg g -1 extract respectively. In our study, unlike the study of Alper et al,quinic acid,fumaric acid,protocatechuic acid,chlorogenic acid,protocatechuic aldehyde,vanillin,ferulic acid,coumarin,salicylic acid,cyranoside,cosmosiin,astragalin,luteolin,apigenin,chrysin and acacetin were found as 44.029,0.267,0.491,16.994,0.024,0.048,0.151,0.05,0.036,0.805,0.854,0.8,0.003,0.157,0.025 and 0.086 mg g -1 extract respectively.…”

“…Taştan et al, (2022) reported DPPH radical scavenging activities in methanolic extract of three different Centaurea species as IC 50 of 55.82, 17.18 and 36.71 μg mL -1 , respectively. In a study by Alper et al, (2021) DPPH radical scavenging IC 50 values of Centaurea solstitialis were found to be 55.04 mg trolox equivalents g -1 for ethanolic extract. In this study, ABTS scavenging activity was found to be 97%, 26% and 94% for Centaurea solstitialis, 98%, 76% and 94% for Mentha pulegium and 98%, 41% and 94% for Lepidium draba in acetone, n-hexane and methanol extracts respectively (Figure 3).…”

“…The total phenolic substance amounts in the methanol extract of Centaurea species are 182 460, 40 2080 and 24 3910 μg GAE g -1 dry matter respectively (Taştan et al, 2022). In the study conducted by Alper et al, they found the total amount of phenolic substance in the ethanolic extract of Centaurea solstitialis as 52 310 μg GAE g -1 extract (Alper et al, 2021).…”

Cytotoxic effect of Centaurea solstitialis L. (Asteraceae) on cell cultures

“…In other words, HeLa and Daudi cells were found to be the most sensitive cell lines in terms of cytotoxicity. 24 In 2021, Alper et al 25 aimed to investigate the phenolic composition and antioxidant activity of C. solstitialis and Urospermum picroides and evaluate their possible cytotoxic effects. RP-HPLC analysis was used to elucidate the phenolic profiles of ethanolic extracts of the flowering parts of C. solstitialis and U. picroides.…”

Antibacterial and Cytotoxic Activities of Copper-Functionalized Silsesquioxane 3-n-Propylpyridinium Chloride

“…The supernatant was then removed. For the solubilization of these crystals, 200 μL of dimethyl sulfoxide was added [20]. The spectrophotometric absorbance reading was performed at a wavelength of 550 nm in a μQuant microplate reader (BioTek, Winooski, VT, USA).…”