Antioxidant Properties, Total Phenolic Content and LC-MS/MS Analysis of Mentha Pulegium, Lepidium Draba and Centaurea Solstitialis

Abstract

The Mentha pulegium, Lepidiıum draba and Centaurea solstitialis have traditionally been used in different cultures for the treatment of various diseases. We investigated the total phenolic content analysis, chemical compositions and antioxidant activities of different solvent extracts such as acetone, methanol and n-hexane obtained from the aerial parts of Mentha pulegium, Lepidiıum draba and Centaurea. The amount of total phenolic substance was determined as gallic acid equivalent determine. Also, the LC-MS/MS technique was used to determine the phenolic profiles of each extract. Finally, antioxidant activities of three extracts were determined by DPPH and ABTS methods. The highest total phenolic content for acetone, n-hexane and methanol extracts was found as 99 507, 46 305, and 18 227 μg GAE mL-1 in Centaurea solstitialis plant, respectively. While the major component rosmarinic acid amount in the acetone extract of Mentha pulegium was 128 195 μg analyte g-1 extract, this amount was determined as 780 383 μg analyte g-1 extract in the methanolic extract. The highest DPPH radical scavenging activity was found in the acetone and methanolic extracts of Mentha pulegium as 77% and 79%, respectively. ABTS radical scavenging activity was also found to be 98% and 94% for Mentha pulegium in acetone and methanol extracts, respectively. The antioxidant capacity of the extracts is related to the total amount of phenolic substances.

Cytotoxic effect of Centaurea solstitialis L. (Asteraceae) on cell cultures

“…Total phenolic content analysis, chemical composition, and antioxidant activity of different solvent extracts, such as acetone, methanol, and n-hexane, obtained from the aboveground parts of M. pulegium, L. draba, and C. solstitialis, were investigated. 26 Total phenolic content was determined as gallic acid equivalent; the LC-MS/MS technique was used to determine the phenolic profiles of each extract; and the antioxidant activities of three extracts were determined by DPPH and ABTS methods. The highest total phenolic contents for acetone, n-hexane, and methanol extracts of Centaurea solstitialis were 99 507, 46 305, and 18 227 μg/mL, respectively.…”

“…The antioxidant capacity of the extracts was found to be related to the total amount of phenolic substances. 26 In 2023, Işık et al 27 used nanoparticles obtained from the water extract of C. solstitialis leaves as green adsorbents for the removal of Reactive Red 180 (RR180) and Basic Red 18 (BR18) dyes in the Fenton reaction. Under optimum operating conditions, the nanoparticles showed high performance in the tested dye removal, with more than 98% elimination.…”

Isolation of phenolic compounds from eco‐friendly white bee propolis: Antioxidant, wound‐healing, and anti‐Alzheimer effects

“…Then, 1 mL of ABTS˙ + solution at different concentrations (10, 20, and 40 μg/mL) was added to the sample. The absorbance was recorded 30 min after mixing, and the percentage of radical scavenging was calculated for each concentration compared to the blank sample without scavenger (Necip & Durgun, 2022 ; Re et al., 1999 ).…”