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In this study, anticholinesterase and antioxidant effects of HPL aqueous extracts (HPLAE), HPL methanol extracts (HPLME), EAL aqueous extracts (EALAE) and EAL methanol extracts (EALME) obtained from Hypericum perforatum L. (HPL) and Equisetum arvense L. (EAL) were investigated. The HPLME fraction on acetylcholinesterase (AChE) showed an inhibitory effect, while others showed no inhibitory effect. Antioxidant activities of different fractions of HPL and EAL were determined using different in vitro methods including Fe 3+-Fe 2+ reduction capacity, ABTS• + radical scavenging capacity, DPPH • free radical reduction capacity, and CUPRAC methods. In the study, the fractions were compared with the standard antioxidant BHT, BHA, and Trolox. The fractions obtained from these plants have 52% radical scavenger activity close to standards, and moderate metal reduction activity. As a result, different fractions of these medicinal plants used to treat many diseases caused by oxidative stress have varying bioactivities.