Assessing paternities with inconclusive STR results: The suitability of bi-allelic markers

Pinto, Nádia; Magalhães, Marta; Conde-Sousa, Eduardo; Gomes, Cláudia; Pereira, Rui; Alves, Cı́ntia; Gusmão, Leonor; Amorim, António

doi:10.1016/j.fsigen.2012.05.002

Cited by 33 publications

(19 citation statements)

References 18 publications

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“…No redundant multilocus genotypes were identified, which indicates that no clones were present in our dataset. All loci were then tested for departure from Hardy-Weinberg equilibrium using the hw.test function of the "pegas" package in R (Paradis et al, 2013). Significance was tested using 1,000 random permutations.…”

Section: Genetic Data and Bioinformaticsmentioning

confidence: 99%

Partitioning genetic and species diversity refines our understanding of species–genetic diversity relationships

Pfeiffer

Ford

Housset

et al. 2018

Ecology and Evolution

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Disentangling the origin of species–genetic diversity correlations (SGDCs) is a challenging task that provides insight into the way that neutral and adaptive processes influence diversity at multiple levels. Genetic and species diversity are comprised by components that respond differently to the same ecological processes. Thus, it can be useful to partition species and genetic diversity into their different components to infer the mechanisms behind SGDCs. In this study, we applied such an approach using a high‐elevation Andean wetland system, where previous evidence identified neutral processes as major determinants of the strong and positive covariation between plant species richness and AFLP genetic diversity of the common sedge Carex gayana. To tease apart putative neutral and non‐neutral genetic variation of C. gayana, we identified loci putatively under selection from a dataset of 1,709 SNPs produced using restriction site‐associated DNA sequencing (RAD‐seq). Significant and positive relationships between local estimates of genetic and species diversities (α‐SGDCs) were only found with the putatively neutral loci datasets and with species richness, confirming that neutral processes were primarily driving the correlations and that the involved processes differentially influenced local species diversity components (i.e., richness and evenness). In contrast, SGDCs based on genetic and community dissimilarities (β‐SGDCs) were only significant with the putative non‐neutral datasets. This suggests that selective processes influencing C. gayana genetic diversity were involved in the detected correlations. Together, our results demonstrate that analyzing distinct components of genetic and species diversity simultaneously is useful to determine the mechanisms behind species–genetic diversity relationships.

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Section: Genetic Data and Bioinformaticsmentioning

confidence: 99%

Partitioning genetic and species diversity refines our understanding of species–genetic diversity relationships

Pfeiffer

Ford

Housset

et al. 2018

Ecology and Evolution

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“…However, supplementary STRs are required to significantly evaluate pairwise, distant and/or complex relationships [3][4][5]. Additionally, additional STRs may be needed to confirm paternity in cases in which genetic mutations occur between the parents and progeny [6], null alleles are present [7] or a relative of an alleged father is involved [8][9][10]. All DNA samples were amplified using the Microreader TM 23sp ID System following the manufacturer's protocol.…”

Section: Dear Editormentioning

confidence: 99%

Genetic polymorphism of 22 autosomal STR markers in a Han population of Southern China

Liu¹,

Chen²,

Chen³

et al. 2016

Forensic Science International: Genetics

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“…Opublikowano także kilka opracowań naukowych prezentujących wyniki symulacji z wykorzystywaniem autosomalnych SNP jako uzupełniających lub głównych markerów w badaniach pokrewieństwa [9,21,26,27]. Dostępne są jednak tylko nieliczne prace opisujące przypadki analizy ojcostwa, w których obliczono wartości LR lub wskaźnika ojcostwa (PI) przed i po zastosowaniu analizowanych SNP [19,28,29]. W niektórych z tych doniesień badano indele należące do innej klasy SNP [30].…”

Section: Introductionunclassified

SNPs and STRs in forensic medicine. A strategy for kinship evaluation

Pontes¹,

Sousa²,

Medeiros³

2017

amsik

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Some emerging technologies are used as strategies for the analyses of single nucleotide polymorphisms (SNPs) that have attracted much interest in recent years, applied to various scientific areas. They have been extensively used as markers to identify genes that underlie complex diseases and also to realize the potential of pharmacogenomics in relation to different drug responses. Additionally, SNPs have been shown to be very useful in forensic genetics resolving all kinds of legal problems, namely crime cases, disaster victim identification and paternity and kinship investigation testing. The low mutation rate of SNPs, makes these markers very suitable for relationship testing. In the great majority of the cases, analyses with the widely used sets of STR markers provide powerful statistical evidence but some of them remain with ambiguous results. Those include cases with complex pedigrees or cases with some problems, like mutations, that are inherent to the use of STRs. At this time several forensic laboratories are using SNPs especially to complement the study of STRs in some of their casework cases. This paper intends to analyze some of our casework examples and to providea data update on the joint use of STRs and autosomal SNPs in the evaluation and kinship calculation, one of the strategies currently used for this purpose, namely reviewing and comparing results published by various working groups.

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Assessing paternities with inconclusive STR results: The suitability of bi-allelic markers

Cited by 33 publications

References 18 publications

Partitioning genetic and species diversity refines our understanding of species–genetic diversity relationships

Partitioning genetic and species diversity refines our understanding of species–genetic diversity relationships

Genetic polymorphism of 22 autosomal STR markers in a Han population of Southern China

SNPs and STRs in forensic medicine. A strategy for kinship evaluation

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