Assessing oral brushing technique as a source to collect DNA and its use in detecting human papillomavirus

Prevalence of oral and oropharyngeal human papillomavirus infection in Brazilian population studies: a systematic review

“…Lira (2010) 25 Mean of 65 y ------49/26 Oliveira (2008) 26 Mean of 59 y 81.6% ---73/14 Simonato (2008) 27 79.3% under 60 y 89.7% 62.1% 27/2 Soares (2008) 28 Mean of 65 y ---------Oliveira (2009) 29 59% over 60 y ------57/31 Pereira (2011) 30 Mean of 63 y ------19/8 Spindula-Filho (2011) 31 55.3% over 65 y 72.3% 61.7% 34/13 Cordeiro-Silva (2012) 32 44.5% over 58 y 80.0% 68.9% 35/10 Kaminagakura (2012) 33 Mean of 51 y 84.0% 62.0% 81/33 Marques-Silva (2012) 34 33 38 Mean of 45 y ------21/43 Ribeiro (2014) 39 Mean of 32 y 27.4% ---31/31 Vidotti (2014) 40 42 Mean of 29 y ------30/0 Peixoto (2011) 43 Mean of 37 70 3 (4.3%) ---------Sacramento (2006) 6 50 7 (14.0%) 1 (2.0%) 1 (2.0%) 2 (4.0%) Silva (2007) 16 10 1 (10.0%) ---------Esquenazi (2010) 7 100 0 (0.0%) ---------Horewicz (2010) 8 104 0 (0.0%) 0 (0.0%) ------Araujo (2011) 45 16 1 (6.2%) 0 (0.0%) 0 (0.0%) 0 (0.0%) Betiol (2012) 13 22 16 16 (100.0%) ---------Mazon (2011) 17 18 6 (33.0%) 0 (0.0%) 0 (0.0%) 0 (0.0%) Fregonezi (2012) 23 Oral/oropharyngeal HPV infection in patients with oral or oropharyngeal SCC Miguel (1998) 24 28 4 (14.3%) ---------Soares (2002) 18 10 0 (0.0%) ---------Soares (2003) 19 27 13 (48.1%) ------10 (37.0%) Fregonesi (2003) 20 17 6 (35.3%) ------5 (29.0%) Cortezzi (2004) 15 16 4 (25.0%) 4 (25.0%) ------Silva (2007) 16 50 37 (74.0%) ---------Soares (2007) 27 29 5 (17.2%) --------...…”

Low rate of oropharyngeal human papillomavirus infection of women with cervical lesions and their partners: new data from Brazilian population

“…According to the method previously described by this group (21), biological material samples were collected with brushing techniques, using sterile cytology brush and 1 ml storage and transport media vial (Digene Corporation, Gaithersburg, MD, USA). Collection technique consisted of 10 push/pull movements in the oropharyngeal mucosa bilaterally, specifically in the soft palate, tonsillar pillars, and tonsils.…”

Oropharynx HPV status and its relation to HIV infection

“…Regarding to methods of sample collection, many procedures are reported for cell collection such as mouth rinsing, saliva collection, scrape and cytobrush ( Donà et al, 2014 ; Marks et al, 2014 ; Beachler et al, 2013 ; Fakhry et al, 2011 ; Fuller et al, 2015 ; Marques et al, 2013 ). In our view, scraping with flexible brush allows better sample collection from a specific anatomic location (oropharyngeal mucosa-posterior and anterior pharyngeal walls, tonsils, soft palate, and tongue base).…”