Assembly of Gold Nanorods on HSA Amyloid Fibrils to Develop a Conductive Nanoscaffold for Potential Biomedical and Biosensing Applications

Abstract

Today, Gold Nanorods have promised variety of applications in conjugation with biomolecules of interest. Discovery of functional amyloids has also been highlighted with possible use in designing high performance materials. To exploit dual properties of both Nano and Bio counterparts in new functional materials, this effort has focused on synthesis of a potential hybrid system of Gold nanorods (GNRs) and HSA amyloid fibrils to develop a conductive nanoscaffold. UV-Vis spectroscopy, Thioflavin T (ThT) assay, Far-UV Circular Dichroism (CD) spectropolarimetry, fluorescence and Transmission Electron microscopy were used to characterize formation of the nanostructures and amyloid fibrils. Surface plasmon resonance of GNRs was also monitored upon interaction with HSA amyloid fibrils, showing that the plasmonic component of the hybrid system has maintained its characteristic rod morphology without any perturbations. Analysis of Nyquist plots for the hybrid nanoscaffold showed that the electronic behavior of the hybrid system has been enhanced due to the presence of the assembled GNRs. Results of this investigation highlight the possibility of fabricating hybrid nano-bioscaffolds as promising candidates in versatile biomedical and biosensing applications.

Surface Functionalization of Gold Nanorods Improves Nanostructure Assemblies on Amyloid Fibril Scaffolds

“…Our group has recently reported synthesis and characterization of assembled gold nanorods on HSA amyloid fibrils. 29 Therefore, the fibrillar biotemplate with evidenced mechanical and chemical stability can properly compensate the fibrillar pattern of CNTs and assembly of GNRs can retrieve its conductivity.…”

Nanotechnology‐Based Strategies for Early Diagnosis of Central Nervous System Disorders

“…[ 194 ] Taheri et al reported a AuNPs conjugated amyloid fibrils based conductive nanoscaffold for PD biosensing. [ 195 ] Recently, Liu et al developed scFv antibodies (called W20) conjugated to PEGylated SPIONs of 12 nm size, targeting oligomers for PD diagnosis with advanced BBB bypassing ability and higher specificity in the oligomer area of PD brain compared with healthy mice. [ 196 ]…”

“…[194] Taheri et al reported a AuNPs conjugated amyloid fibrils based conductive nanoscaffold for PD biosensing. [195] Recently, Liu et al developed scFv antibodies (called W20) conjugated to PEGylated SPIONs of 12 nm size, targeting oligomers for PD diagnosis with advanced BBB bypassing ability and higher specificity in the oligomer area of PD brain compared with healthy mice. [196] Surface-based fluorescence intensity distribution analysis (sFIDA) is a type of fluorescent immunoassay with a higher sensitivity for quantifying single AβO proteinaceous NPs in body fluids such as CSF and plasma.…”

Multi‐walled Carbon Nanotubes and Gold Nanorod Decorated Biosensor for Detection of microRNA‐126

“…They could be manufactured in different aspect ratios, which could alter their optical properties [13]. Moreover, they have high stability and could be prepared with self‐assembly techniques [13a].…”