Assay of Coenzyme Q10 in Plasma by a Single Dilution Step

Confirmation of oxidative stress and fatty acid disturbances in two further Papillon–Lefèvre syndrome families with identification of a new mutation

“…CoQ and vitamin E were assayed by means of HPLC methods assessed as modification of methods that we successfully developed in the last years . The HPLC system consisted of a Beckman model 126 pump (Beckman Coulter, Fullerton, CA, USA), a Rheodyne model 7125 valve (Rheodyne, Cotati, CA, USA) fitted with a 20 μL loop, a stainless steel column 15 cm long 4.6 mm I.D.…”

In Stored Human Blood, The Inhibitor Effect of Tannic Acid and Caffeic Acid on Lipid Peroxidation and Oxidative DNA Damage

“…The obtained results are expressed as μM. Oxide CoQ10 Analysis: Oxidized CoQ10 analysis was performed according to the method by Littarru et al (15) The reductive CoQ10 was forced to oxidize in plasma samples treated with para-benzoquinone following extraction with 1-propanol directly injected into the HPLC device. For HPLC analysis, a reverse-phase ODS supercoil C-18 (15x0.46 cm i.d.…”

Actions of resveratrol and serotonin added to stored bloods on lipid peroxidation and oxidative DNA damage

“…The obtained results are expressed as µM. Analysis of Oxidized CoQ10: Oxidized CoQ10 levels were measured according to the method previously described by Littarru et al (28). Briefly, reduced CoQ10 was forced to oxidize in plasma samples treated with para-benzoquinone following extraction with 1-propanol directly injected into the HPLC device.…”