Asiatic clam Corbicula fluminea exhibits distinguishable behavioural responses to crude oil under semi-natural multiple stress conditions

Distribution of oil products in bottom sediments of the Irtysh River in the urban area

“…It was established that under the influence of toxic substances, including oil products in hydroecosystems, changes occur at all levels of the organization: from molecular to ecosystem, significant in terms of environmental consequences for surface water bodies: molecular -changes in the conformation and activity of enzymes, chromosomal and gene mutations, dysregulation of cellular metabolism, depletion of glycogen depots, hyperglycemia, weakened immunity, impaired lipid, protein and energy metabolism, etc. [12,[17][18][19][20][21][22][23][24];…”

Proteome changes in muscles, ganglia, and gills in Corbicula fluminea clams exposed to crude oil: Relationship with behavioural disturbances

“…The artificial stream facility is an outdoor experimental site exposed to natural variations and supplied with freshwater from the Gave de Pau River. The water is derived upstream of a dam, flows by gravity in a flow control system, passes through a living organism "nursery" and is identically distributed in 16 experimental streams (length, 40 m; width, 0.5 m; depth, 0.5 m; water depth, 0.25 m) arranged in parallel (Cailleaud et al, 2019;Miserazzi et al, 2020). At the beginning of streams, a piston pump (Prominent) coupled to a high-pressure pump and a shearing valve (Netzsch; Nemo) allows to continuously inject and disperse crude oil in water as micro-droplets.…”

“…Clams used for tissue sampling were in a second sand zone, located at 29-30 m from the crude oil injection (n = 5 clams per stream for proteomic analysis, see section 2.7 for details; n = 5 clams per stream for contamination analysis, see section 2.8 for details). The spacing between the two zones made it possible to sample in the second zone without disturbing clams in the first zone (Miserazzi et al, 2020).…”

“…Protein identification and quantification of individuals subjected to different situations, completed by functional annotations and pathway analysis of proteins, are powerful tools providing access to a global or detailed view of altered biological processes (Campos et al, 2012;Lemos et al, 2009;Van Aggelen et al, 2010). This study was performed on the freshwater bivalve Corbicula fluminea in a semi-natural environment within outdoor artificial streams during the first 3 d of crude oil exposure (Miserazzi et al, 2020). Relatively few studies on the effects of crude oil have been carried out in freshwater environments (Bhattacharyya et al, 2003;Green and Trett, 1989;Monteiro et al, 2019).…”

“…Finally, based on this set of indicators, behaviour, PAH contamination and proteomic analysis, we propose a couple of explanatory mechanisms linking behaviour and disturbance of the internal medium in crude oil exposed bivalve molluscs. This report is the sister report of a recent work on distinguishable behavioural responses to crude oil in Asian clams Corbicula fluminea under semi-natural multiple stress conditions (Miserazzi et al, 2020). In brief, to mimic field conditions and for the purposes of the present experiment, clams were exposed in two outdoor artificial streams that were subject to natural variations and were continuously fed by freshwater from the Gave de Pau river (S.W.…”