Proteome changes in muscles, ganglia, and gills in Corbicula fluminea clams exposed to crude oil: Relationship with behavioural disturbances

Miserazzi, Alison; Perrigault, Mickael; Sow, Mohamedou; Gelber, Clémentine; Ciret, Pierre; Lomenech, Anne-Marie; Dalens, J.M.; Weber, C.; Floch, Stéphane Le; Blanc, Philippe; Massabuau, Jean‐Charles

doi:10.1016/j.aquatox.2020.105482

Cited by 8 publications

(3 citation statements)

References 97 publications

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“…Furthermore, biomonitoring surveys must be improved by understanding the physiological changes associated with behavioral monitoring systems. Proteomic analysis has been utilized for clam-based model species [89]. For example, the grazing behavior of aquatic insects is a mechanism that affects algae communities (bottomup processes) [30].…”

Section: Proteomicsmentioning

confidence: 99%

Current Status of Omics in Biological Quality Elements for Freshwater Biomonitoring

Machuca-Sepúlveda

Miranda

Lefin

et al. 2023

Biology

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Freshwater ecosystems have been experiencing various forms of threats, mainly since the last century. The severity of this adverse scenario presents unprecedented challenges to human health, water supply, agriculture, forestry, ecological systems, and biodiversity, among other areas. Despite the progress made in various biomonitoring techniques tailored to specific countries and biotic communities, significant constraints exist, particularly in assessing and quantifying biodiversity and its interplay with detrimental factors. Incorporating modern techniques into biomonitoring methodologies presents a challenging topic with multiple perspectives and assertions. This review aims to present a comprehensive overview of the contemporary advancements in freshwater biomonitoring, specifically by utilizing omics methodologies such as genomics, metagenomics, transcriptomics, proteomics, metabolomics, and multi-omics. The present study aims to elucidate the rationale behind the imperative need for modernization in this field. This will be achieved by presenting case studies, examining the diverse range of organisms that have been studied, and evaluating the potential benefits and drawbacks associated with the utilization of these methodologies. The utilization of advanced high-throughput bioinformatics techniques represents a sophisticated approach that necessitates a significant departure from the conventional practices of contemporary freshwater biomonitoring. The significant contributions of omics techniques in the context of biological quality elements (BQEs) and their interpretations in ecological problems are crucial for biomonitoring programs. Such contributions are primarily attributed to the previously overlooked identification of interactions between different levels of biological organization and their responses, isolated and combined, to specific critical conditions.

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Section: Proteomicsmentioning

confidence: 99%

Current Status of Omics in Biological Quality Elements for Freshwater Biomonitoring

Machuca-Sepúlveda

Miranda

Lefin

et al. 2023

Biology

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“…Several monitoring and early warning systems, based on valvometry measurements, have been described in the literature (e.g., [8,23,[45][46][47][48][49], see also Table A2); additionally, a few systems have been commercialized (Table A1). The system that we proposed in this study is, to the best of our knowledge, the first to be built on a biophysical model of the adductor muscle dynamics.…”

Section: A System Based On Quantification Of the Valve Gape Dynamicsmentioning

confidence: 99%

Designing the Next Generation of Condition Tracking and Early Warning Systems for Shellfish Aquaculture

Guarini¹,

Hinz²,

Coston‐Guarini³

2021

JMSE

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Early detection of environmental disturbances affecting shellfish stock condition is highly desirable for aquaculture activities. In this article, a new biophysical model-based early warning system (EWS) is described, that assesses bivalve stock condition by diagnosing signs of persistent physiological dysfunctioning. The biophysical model represents valve gape dynamics, controlled by active contractions of the adductor muscle countering the passive action of the hinge ligament; the dynamics combine continuous convergence to a steady-state interspersed with discrete closing events. A null simulation was introduced to describe undisturbed conditions. The diagnostic compares valve gape measurements and simulations. Indicators are inferred from the model parameters, and disturbances are assessed when their estimates deviate from their null distribution. Instead of focusing only on discrete events, our EWS exploits the complete observed dynamics within successive time intervals defined by the variation scales. When applied to a valvometry data series, collected in controlled conditions from scallops (Pecten maximus), the EWS indicated that one among four individuals exhibited signs its physiological condition was degrading. This was detected neither during experiments nor during the initial data analysis, suggesting the utility of an approach that quantifies physiological mechanisms underlying functional responses. Practical implementations of biological-EWS at farming sites are then discussed.

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“…Proteome rearrangements are common responses to environmental stress, so they must be distinctive of certain types of toxic exposure [ 28 ]. Currently, proteomic studies conducted on bivalves have been focused primarily on protein expression profile analyses after exposure to a variety of environmental contaminants [ 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. Others have investigated the response to ocean acidification [ 38 , 39 , 40 , 41 ] and evaluated the effect of toxic phytoplankton [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Species of Prorocentrum Genus on the Japanese Oyster Crassostrea gigas Proteomic Profile

Matus-Hernández

Saavedra

2021

Toxins

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This paper assesses the effects of exposure to toxic concentrations (1200 to 6000 cells/mL) of the dinoflagellates Prorocentrum lima, Prorocentrum minimum, and Prorocentrum rhathymum and several concentrations of aqueous and organic extracts obtained from the same species (0 to 20 parts per thousand) on the Crassostrea gigas (5–7 mm) proteomic profile. Through comparative proteomic map analyses, several protein spots were detected with different expression levels, of which eight were selected to be identified by liquid chromatography–mass spectrometry (LC–MS/MS) analyses. The proteomic response suggests that, after 72 h of exposure to whole cells, the biological functions of C. gigas affected proteins in the immune system, stress response, contractile systems and cytoskeletal activities. The exposure to organic and aqueous extracts mainly showed effects on protein expressions in muscle contraction and cytoskeleton morphology. These results enrich the knowledge on early bivalve developmental stages. Therefore, they may be considered a solid base for new bioassays and/or generation of specific analytical tools that allow for some of the main effects of algal proliferation phenomena on bivalve mollusk development to be monitored, characterized and elucidated.

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Proteome changes in muscles, ganglia, and gills in Corbicula fluminea clams exposed to crude oil: Relationship with behavioural disturbances

Cited by 8 publications

References 97 publications

Current Status of Omics in Biological Quality Elements for Freshwater Biomonitoring

Current Status of Omics in Biological Quality Elements for Freshwater Biomonitoring

Designing the Next Generation of Condition Tracking and Early Warning Systems for Shellfish Aquaculture

Effect of Different Species of Prorocentrum Genus on the Japanese Oyster Crassostrea gigas Proteomic Profile

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