“…Examining the interactions among the different film components (carbohydrates and protein) and their microstructures allows us to obtain a better idea of film applications. New tools in nanotechnology, such as nanoindentation, atomic force microscopy [10], and ellipsometry [11], as well as traditional techniques, such as X-ray diffraction [12], scanning electron microscopy [13], texture [14] and colour analysis [15], are among the methods used to characterise edible films. The aim of this work was to characterise edible films made from chitosan, zein and their mixtures by applying new technologies, such as ellipsometry, nanoindentation, atomic force microscopy, Raman spectroscopy, and classical film analyses (X-ray diffraction analysis, water vapour permeability) to explore how these properties change as a result of protein addition.…”