Ascorbic acid potentiates mitomycin C-induced micronuclei and sister chromatid exchanges in human peripheral blood lymphocytes in vitro

Krishnaja, A.P.; Sharma, N. K.

doi:10.1002/tcm.10064

Cited by 20 publications

(23 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, Dusre et al 20) reported that mitomycin C (MMC) induced not only DNA damage (alkylation and DNA DSB) but also stimulated oxy-radical formation. It is reported that ascorbic acid, an antioxidant chemical, decreased the frequency of micronuclei induced by MMC 21) . It is considered that the mechanism of micronucleus induction may therefore involve free radicals.…”

Section: Discussionmentioning

confidence: 99%

Indium Chloride‐induced Micronuclei in In Vivo and In Vitro Experimental Systems

Takagi¹,

Suzuki²,

Seki³

et al. 2011

Journal of Occupational Health

View full text Add to dashboard Cite

Indium Chloride-induced Micronuclei inIn Vivo and In Vitro Experimental Systems: Ryo Takagi, et al. Department of Public Health and Environmental Medicine, Jikei University School of Medicine-Objectives: The aim of this study was to investigate the genotoxic effects of indium trichloride (InCl 3 ·4H 2 O; InCl 3 ) using the in vivo bone marrow micronucleus test and the in vitro CHL/IU cell micronucleus test. Method: BALB/c mice were administered a single intraperitoneal (i.p.) injection of InCl 3 at a dose 0.625, 1.25, 2.5, 5, or 10 mg/kg b.w. The frequency of micronuclei, the ratio of polychromatic erythrocytes to normochromatic erythrocytes (P/N ratio) and body weight gain were determined 24 h after administration of the InCl 3. In the in vitro micronucleus test, CHL/IU cells were treated continuously for 24, 48, or 72 h in the absence of S9mix (the continuous treatment method) and/or for 6 h with or without S9 mix followed by an 18, 42 or 66 h recovery time (the short time treatment method). The frequency of micronuclei was determined at the end of each culture period. Results: The frequency of micronuclei induced by InCl 3 increased in the in vivo erythroblast-erythrocyte micronucleus test using BALB/c mice at doses of 2.5 and 5 mg/kg b.w. The P/N ratio, a marker of bone marrow toxicity, decreased significantly following the injection of InCl 3 . Body weight gain was also inhibited by InCl 3 . InCl 3 induced micronuclei in the CHL/IU cell micronucleus test in both the continuous treatment method and the short time treatment method, both with and without S9mix. Conclusions: These results suggest that InCl 3 has a genotoxic effect on mammalian cells both in vivo and in vitro. (J Occup Health 2011; 53: 102-109)

show abstract

Section: Discussionmentioning

confidence: 99%

Indium Chloride‐induced Micronuclei in In Vivo and In Vitro Experimental Systems

Takagi¹,

Suzuki²,

Seki³

et al. 2011

Journal of Occupational Health

View full text Add to dashboard Cite

show abstract

“…Finally, slides rinsed in ice-cold Sorensen's buffer, pH 6.8, followed by tap water were stained with 4% Giemsa (Merck) in Sorensen's buffer, pH 6.8. The optimum staining time varied between slide and stain batches, but was of the order of~8 min (Krishnaja and Sharma, 2003). Harlequin staining of BrdUsubstituted chromatids by FPG staining allowed discriminative scoring of chromosomal aberrations in the ®rst, second, third, fourth and ®fth mitoses after irradiation.…”

Section: Methodsmentioning

confidence: 99%

Transmission of -ray-induced unstable chromosomal aberrations through successive mitotic divisions in human lymphocytes in vitro

Krishnaja

Sharma

2004

Mutagenesis

Self Cite

View full text Add to dashboard Cite

Transmission of unstable chromosomal aberrations through successive mitotic divisions M1±M4 was analysed in 3 Gy g-ray-irradiated G 0 human lymphocytes in vitro, harvested at 50, 72 and 96 h. Bromodeoxyuridine (BrdU) incorporation and subsequently the¯uorescence plus Giemsa (FPG) method allowed the cell cycle status of each cell scored to be ascertained. Higher dicentric frequencies were observed in cells within the same post-irradiation division derived from extended culture times, indicating either a delay in cell cycle progression of aberrant cells or different lymphocyte sub-populations. The yield of complete dicentrics decreased by a factor of two in passing from M1 to M2 and showed further reductions of 26 and 44%, respectively, in moving from M2 to M3 and from M3 to M4. In the case of conventionally stained metaphases, wherein the cell cycle status does not enter the picture, the dicentric frequencies showed a reduction in yield of 39.6% at 72 h and 52.1% at 96 h compared with 50 h, because the cells analysed comprise a mixture of metaphases in different cell cycles. In the FPG stained ®rst division metaphases, 92±100% of dicentrics analysed at 50, 72 and 96 h and in the conventionally stained metaphases, 90±94, 72±84 and 54±80% of dicentrics analysed at 50, 72 and 96 h respectively were complete dicentrics (with fragments). In the M1, M2, M3 and M4 metaphases analysed, 92±100, 50±89, 20±70 and 10±50% of dicentrics, respectively, were complete dicentrics and 55±75, 53±68, 43±57 and 36±50% excess acentrics, respectively, were seen in cells with complete dicentrics. Interindividual variation was observed in cell cycle kinetics, radiation-induced chromosomal aberrations and micronucleus frequencies. A salient feature of the delayed effects was the induction of giant cells and the mirror dicentrics observed in them. The reduction in dicentric frequencies due to either cell cycle delay or cell death in successive generations is aided by the multiplicity of aberrations. Bridge±breakage±fusion (BBF) events mediated by dicentric chromosomes may also be instrumental in the perpetuation of chromosomal instability.

show abstract

“…L-ascorbic acid pretreatment caused a statistically significant increase in MMCinduced micronuclei (MN) and SCE frequencies for all treatment groups, but it did not show an increase in chromosome aberrations compared with MMC treatment alone (Krishnaja and Sharma 2003). This study indicates modulatory effect of L-ascorbic acid on MMC-induced chromosome damage.…”

Section: Discussionmentioning

confidence: 73%

Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes

et al. 2006

View full text Add to dashboard Cite

In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the first, only 10 -5 , 10 -4 , 10 -3 and 10 -2 ll ml -1 concentrations of TE were used for 48 h to detect potential clastogenicity. In the second, MMC (0.38 lg ml -1 ) plus 10 -5 , 10 -4 , 10 -3 and 10 -2 ll ml -1 concentrations of TE were used for 48 h to determine anticlastogenic effects. TE did not increase sister chromatid exchanges (SCEs) (except 10 -2 ll ml concentration) and chromosome aberrations (CAs) significantly compared with negative and solvent controls. However, it decreased the frequency of MMC induced chromosome aberrations. Decreasing was significant at 10 -4 , 10 -3 and 10 -2 ll ml -1 concentrations. On the other hand, TE significantly increased MMC-induced SCEs for all treatment groups compared with positive control.

show abstract

Ascorbic acid potentiates mitomycin C-induced micronuclei and sister chromatid exchanges in human peripheral blood lymphocytes in vitro

Cited by 20 publications

References 45 publications

Indium Chloride‐induced Micronuclei in In Vivo and In Vitro Experimental Systems

Indium Chloride‐induced Micronuclei in In Vivo and In Vitro Experimental Systems

Transmission of -ray-induced unstable chromosomal aberrations through successive mitotic divisions in human lymphocytes in vitro

Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes

Contact Info

Product

Resources

About