Effects of Thymus kotschyanus var. glabrescens Boiss. extract on mitomycin-C induced chromosomal aberrations and sister chromatid exchanges in human lymphocytes

Abstract

In this investigation, clastogenic effects of Thymus kotschyanus var. glabrescens Boiss. extract (TE) and anticlastogenic effects of this extract against Mitomycin C (MMC) induced chromosome damage have been evaluated in human peripheral blood lymphocytes in vitro. Two series of experiments were conducted. In the first, only 10 -5 , 10 -4 , 10 -3 and 10 -2 ll ml -1 concentrations of TE were used for 48 h to detect potential clastogenicity. In the second, MMC (0.38 lg ml -1 ) plus 10 -5 , 10 -4 , 10 -3 and 10 -2 ll ml -1 concentrations of TE were used for 48 h to determine anticlastogenic effects. TE did not increase sister chromatid exchanges (SCEs) (except 10 -2 ll ml concentration) and chromosome aberrations (CAs) significantly compared with negative and solvent controls. However, it decreased the frequency of MMC induced chromosome aberrations. Decreasing was significant at 10 -4 , 10 -3 and 10 -2 ll ml -1 concentrations. On the other hand, TE significantly increased MMC-induced SCEs for all treatment groups compared with positive control.