Ascorbic acid content in leaves of Nightshade (Solanum spp.) and spider plant (Cleome gynandra) varieties grown under different fertilizer regimes in Western Kenya

Ayua, Emmanuel; Mugalavai, Violet; Simon, James E.; Weller, Stephen C.; Obura, Pamela; Nyabinda, Naman

doi:10.5897/ajb2015.14936

Cited by 12 publications

(1 citation statement)

References 7 publications

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“…It is because the ascorbic acid transports from the mature leaves to the immature leaves because younger leaves require ascorbic acid for cell division and growth. It is consistent with the statement of Ayua et al, (2016), that the young leaves require more vitamin C, but ascorbic acid synthesis cannot meet its physiological processes. It is in line with the statement of Davey et al (2006) that ascorbic acid plays a role in cell growth and the process of photosynthesis.…”

Section: Resultssupporting

confidence: 92%

The Content of Chlorophyll, and Antioxidant Activity of Malabar plum (Syzygium jambos) Leaves at Different Developmental Stages

2019

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Malabar plum [Syzygium jambos (L.) Alston.] is a tropical plant which is used as a medicinal plant, because it contains secondary metabolites, especially in the leaves. The different leaves developmental stages can affect physiological changes, especially metabolic processes, so it is suspected to affect the antioxidant content and activity. The objective of this research was to study the difference of leaves morphology, chlorophyll contents, antioxidant contents, and activity at the different leaves developmental stages. Samples were taken from Kaliboto Village, Purworejo, Central Java. The leaf color measurement was using colorimetry; determination of chlorophyll, carotenoids, ascorbic acid, and total phenol content is was using spectrophotometry; and antioxidant activity was using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Qualitative data were analyzed descriptively, while quantitative data were analyzed by ANOVA tests. The results showed that the higher level of leaves development, the higher pigment content, total phenol, and antioxidant activity ascorbic acid content in the mature leaves is lower when compared to the young and old leaves. The results of this research provide the information that can support the use of Malabar plum leaves in traditional medicinal activity and pharmaceutical industry, as well as basic information for plant breeding.

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Section: Resultssupporting

confidence: 92%

The Content of Chlorophyll, and Antioxidant Activity of Malabar plum (Syzygium jambos) Leaves at Different Developmental Stages

2019

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Ten Years ofGynandropsis gynandraResearch for Improvement of Nutrient‐Rich Leaf Consumption: Lessons Learnt and Way Forwards

Achigan‐Dako

Sogbohossou

Houdegbe

et al. 2021

Annual Plant Reviews Online

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Article 25fa states that the author of a short scientific work funded either wholly or partially by Dutch public funds is entitled to make that work publicly available for no consideration following a reasonable period of time after the work was first published, provided that clear reference is made to the source of the first publication of the work.This publication is distributed under The Association of Universities in the Netherlands (VSNU) 'Article 25fa implementation' project. In this project research outputs of researchers employed by Dutch Universities that comply with the legal requirements of Article 25fa of the Dutch Copyright Act are distributed online and free of cost or other barriers in institutional repositories. Research outputs are distributed six months after their first online publication in the original published version and with proper attribution to the source of the original publication.

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Rapid screening of glycoalkaloids in Solanum scabrum and S. nigrum berries using ultra‐high‐performance liquid chromatography with pathway‐specified in‐source fragmentation tandem mass spectrometry

Lyu

Yuan

Dinssa³

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale The safe consumption of Solanum scabrum and S. nigrum berries (SNBs) depends on a reliable and rapid chemical screen for the testing of the fruit and/or final food and industrial products for the presence and level of toxic glycoalkaloids. Such a rapid and sensitive screen could also be used by those involved in food safety and forensics, industry, research labs and those in agriculture production, breeding and food processing. Significant variation in the content and composition of glycoalkaloids across SNBs has been reported. To facilitate high‐throughput targeted analysis, this work overcame the slow scan speed of a traditional triple quadruple mass spectrometry (QqQ) method by development of a pseudo‐MS3 method. Methods In‐source fragmentation functioned as a pseudo‐MS or pseudo‐hydrolysis to trim down the structurally diverse and complex glycosides into five types of aglycone ions, which were then analyzed using multiple reaction monitoring (MRM). Characteristic product ions were selected based on the aglycone skeleton and substitution pattern and associated fragmentation pathway. Results A compact method with only 15 MRM transitions were developed for high‐throughput screening of very diverse glycoalkaloids. Glycosides of the same aglycone type were readily identified in the same transition window without the need for mass spectra interpretation. Validated using solamargine, the sole available standard, the accuracy was 99.7–101.3%, the intra‐ and inter‐day precision were, respectively, 2.5–5.0% and 8.0–9.2%, and the lower limit of detection and quantification were, respectively, 3.1 and 10.2 ng/mL (with 1 μL injection volume). Conclusions The peudo‐MS3 method allowed for high‐throughput targeted analysis with compact MRM transitions to address a large number of glycoalkaloids with diverse structures. This method could serve to meet the most heavy‐duty demand for rapid inspection of glycoalkaloids in SNBs. This method can be adopted and used by those involved in food safety and forensics, in developing food and industrial products and in genetics and breeding.

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Ascorbic acid content in leaves of Nightshade (Solanum spp.) and spider plant (Cleome gynandra) varieties grown under different fertilizer regimes in Western Kenya

Cited by 12 publications

References 7 publications

The Content of Chlorophyll, and Antioxidant Activity of Malabar plum (Syzygium jambos) Leaves at Different Developmental Stages

The Content of Chlorophyll, and Antioxidant Activity of Malabar plum (Syzygium jambos) Leaves at Different Developmental Stages

Ten Years ofGynandropsis gynandraResearch for Improvement of Nutrient‐Rich Leaf Consumption: Lessons Learnt and Way Forwards

Rapid screening of glycoalkaloids in Solanum scabrum and S. nigrum berries using ultra‐high‐performance liquid chromatography with pathway‐specified in‐source fragmentation tandem mass spectrometry

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