Artificial skin equivalent based on copolymeric hydrogel membranes with immobilized human mesenchymal stem cells

Abstract

The basic parameters of hydrogel membranes, which are important for their characteristics as potential biocompatible non-degradable wound coverings, have been optimized. Moderately hydrophobic and at the same time moderately hydrophilic membranes obtained on the basis of copolymerization of acrylamide and acrylonitrile were demonstrated to be biocompatible with mesenchymal stem cells. Small concentrations of highly dispersed silica introduced into a composition of hydrogel membranes were demonstrated to influence the cell cultivation positively. The technological conditions necessary for more effective and prolonged cultivation of cells on the surface of hydrogel membranes with improved exploitation parameters have been established.

From mesenchymal stem cells to their secretoms as basic components of dermal coverings for the treatment of massive burns

“…Starting from 2006 until nowadays, on the basis of experimental research data the world's scientists came to the conclusion that mesenchymal stem cells (MSCs) or multipotent mesenchymal stromal cells (MMSCs) and their derivatives, exosomes and secretomes (cultural mediums conditioned by MSCs), are really promising biologically active components for biotechnological wound coverings [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16]. Taking this into account, it was inte res ting for us to compare the activity of human cells of different types and origin in the same experiments.…”

“…In our works [1-4, 12-16, 19-21] we are talking about the creation of new biotechnological dermal coatings: the features of the materials selection for the cellular carrier, as well as cellular components, cells and/or their derivatives, in comparison with known literature data. Polyacrylamide hydrogel was used by us as a first sample of cell carrier for temporary dermal equivalents [1][2][3][4]12]. At the beginning of experimental research in the field of cellular technologies, polyacrylamide hydrogels were not used for the manufacturing of artificial wound coverings.…”

“…Noteworthy that all variants of dense wound coverings, have been described earlier, significantly differed from the bioconstructions that became the object of our work [2][3][4]12]. We intend to create new equivalents of the dermis and conduct their investigation -first laboratory, then preclinical -using an adap ted polyacrylamide hydrogel coating, on the surface of which the immobilized living cells are able to attach and multiply.…”