Abstract:Currently, the development and the implementation of the new biotechnological wound coverings (skin equivalents) designed for temporal or permanent replacement of damaged or destroyed areas of human skin remains extremely actual relevant in clinical practice. Skin equivalents or equivalents of individual skin layers which include alive cells of different types take a special place among the artifi cial wound coverings. They mostly contain two basic types of cells-fi broblasts and keratinocytes (together or sep… Show more
“…Fifty-six percent of patients treated using Apligraft had full wound healing in comparison with 37% of patients who were treated using standard wound care protocols. Figure 4 shows the appearance of a wound before and after application of Apligraft [94].…”
Section: Biodegradable Polymersmentioning
confidence: 99%
“…Fifty-six percent of patients treated using Apligraft had full wound healing in comparison with 37% of patients who were treated using standard wound care protocols. Figure 4 shows the appearance of a wound before and after application of Apligraft [94]. Figure 4. (a) Appearance of wound before application of apligraft; (b) application of apligraft; (c) appearance of wound after application.…”
Section: Organ-specific Regeneration Using Biodegradable Materialsmentioning
Tissue engineering and regenerative medicine are emerging as future approaches for the treatment of acute and chronic diseases. However, many challenging clinical conditions exist today and include congenital disorders, trauma, infection, inflammation and cancer, in which hard and soft tissue damage, organ failure and loss are still not treated effectively. Regenerative medicine has contributed to a number of innovations through artificial implants and biomedical materials, with advances are continually being made. Researchers are constantly developing new biomaterials and tissue engineered technologies to stimulate tissue regeneration in order to repair and replace damaged or malfunctioning organs. However, the challenge continues to lie in devising effective biomedical materials that can be implanted as scaffolds. Various approaches are emerging, according to the organ, tissue, disease and disorder. Scaffolds are implanted cell-free, or incorporated with stems cells, committed cells, or bioactive molecules. Irrespective, engineered biomaterials are required to regenerate and ultimately reproduce the original physiological, biological, chemical and mechanical properties over time. This is enabled by providing a three-dimensional architecture for cells to adhere, migrate, proliferate within, and differentiate appropriately for the growth of new tissues to provide a relevant structure, and in so doing, restore function. Biodegradable materials have been used extensively as regenerative therapies since their advent in early 20th century. One notable example is the development of surgical fixation devices. The selection, design and physicochemical properties of these materials are important and must consider biocompatibility, biodegradability and minimal cytotoxicity in the host to enable cell-proliferation, cell-matrix interactions and intercellular signalling for stimulating tissue growth. In this review, we critique the most studied and recently developed biodegradable
“…Fifty-six percent of patients treated using Apligraft had full wound healing in comparison with 37% of patients who were treated using standard wound care protocols. Figure 4 shows the appearance of a wound before and after application of Apligraft [94].…”
Section: Biodegradable Polymersmentioning
confidence: 99%
“…Fifty-six percent of patients treated using Apligraft had full wound healing in comparison with 37% of patients who were treated using standard wound care protocols. Figure 4 shows the appearance of a wound before and after application of Apligraft [94]. Figure 4. (a) Appearance of wound before application of apligraft; (b) application of apligraft; (c) appearance of wound after application.…”
Section: Organ-specific Regeneration Using Biodegradable Materialsmentioning
Tissue engineering and regenerative medicine are emerging as future approaches for the treatment of acute and chronic diseases. However, many challenging clinical conditions exist today and include congenital disorders, trauma, infection, inflammation and cancer, in which hard and soft tissue damage, organ failure and loss are still not treated effectively. Regenerative medicine has contributed to a number of innovations through artificial implants and biomedical materials, with advances are continually being made. Researchers are constantly developing new biomaterials and tissue engineered technologies to stimulate tissue regeneration in order to repair and replace damaged or malfunctioning organs. However, the challenge continues to lie in devising effective biomedical materials that can be implanted as scaffolds. Various approaches are emerging, according to the organ, tissue, disease and disorder. Scaffolds are implanted cell-free, or incorporated with stems cells, committed cells, or bioactive molecules. Irrespective, engineered biomaterials are required to regenerate and ultimately reproduce the original physiological, biological, chemical and mechanical properties over time. This is enabled by providing a three-dimensional architecture for cells to adhere, migrate, proliferate within, and differentiate appropriately for the growth of new tissues to provide a relevant structure, and in so doing, restore function. Biodegradable materials have been used extensively as regenerative therapies since their advent in early 20th century. One notable example is the development of surgical fixation devices. The selection, design and physicochemical properties of these materials are important and must consider biocompatibility, biodegradability and minimal cytotoxicity in the host to enable cell-proliferation, cell-matrix interactions and intercellular signalling for stimulating tissue growth. In this review, we critique the most studied and recently developed biodegradable
“…However, if the total area of wounds exceeds 30-40 % of the body surface, this method does not allow closing the entire surface of the wound, even when using perforated autodermal grafts. This led to the introduction of various artificial skin substitutes into the arsenal of a combustiologist, among which the best ones are biotechnological wound coverings containing living cells of various types and origins, mainly differentiated fibroblasts and keratinocytes [1].…”
Section: Introductionmentioning
confidence: 99%
“…Such bioconstructions usually serve as temporary equivalents of the skin or its separate layers (dermis, epidermis), which provide the patient's damaged tissues with biologically active substances that stimulate skin regeneration. A huge number of requirements for an ideal skin substitute are listed in the review [1]. But perfect temporary skin substitutes have not yet been invented, so the efforts of biotechnologists around the world are aimed at finding new ways to solve this important task using new approaches and materials.…”
Section: Introductionmentioning
confidence: 99%
“…Biotech nological dermal equivalents are of the particular importance for the treatment of massive burns. One of the main problems in the treatment of burn disease is the need to treat deep and extensive damage of the skin with further stimulation of tissue repair regeneration, as far as it is possible with the capacity of modern medicine [1]. Such wounds have a high risk of infection and often require expensive treatment by skin autotransplantation or autodermoplasty.…”
In the course of our long term research, we have created new biotechnological productsdermal coverings (dermis equivalents) with the inclusion of human MSCs or their secretomes (cells conditioned mediums which contain a complex of biologically active substances synthesized by them). Preclinical studies on the model animals and clinical trials of new dermal equivalents on the limited group of patients with massive burns have been conducted to determine their therapeutic effectiveness and well as safety. We proved at special in vivo experiments that new dermal coverings contribute positively to the healing severe deep burn wounds when applied to the wound surface and don't reveal any toxic effect in the studied organisms. The developed method of obtaining new biotechnological products has been patented.K e y w o r d s: cellular biotechnology, dermal covering or dermis equivalent, mesenchymal stem cell (MSC), conditioned media (CM), secretome, burns.
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