Arthrobacter bussei sp. nov., a pink-coloured organism isolated from cheese made of cow’s milk

Flegler, Alexander; Runzheimer, Katharina; Kombeitz, Vanessa; Mänz, Anna Tatjana; Heilborn, David Heidler von; Etzbach, Lara; Schieber, Andreas; Hölzl, Georg; Hüttel, Bruno; Woehle, Christian; Lipski, André

doi:10.1099/ijsem.0.004125

“…The predominant menaquinones of both strains were MK-9(H 2 ), while MK-8(H 2 ) was present as minor compound in the two strains. Thus, the characteristics of quinones were consistent with those of members of the genus Arthrobacter [2, 28, 29]. The polar lipids detected in two strains were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and unidentified glycolipid (Fig.…”

Section: Physiology and Chemotaxonomysupporting

confidence: 72%

Arthrobacter cheniae and Arthrobacter frigidicola sp. nov., isolated from a glacier

Yang

¹

,

Liu

²

,

Liu

³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-stain-positive, aerobic, rod-shaped, pink and light pink colony-forming bacteria, designated as Hz2T and MDT2-14T, respectively, were isolated from glacier cryoconite samples. Comparisons based on 16S rRNA gene sequences showed that strains Hz2T and MDT2-14T take Arthrobacter bussei KR32T and Arthrobacter zhaoguopingii J391T as their closest neighbours, respectively. The average nucleotide identity values between the two novel strains and their closest relatives were 83.56 and 93.06 %, respectively. The two strains contain MK-9(H2) as their predominant menaquinone. The polar lipids of both strains were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The major fatty acids of strain Hz2T were anteiso-C15 : 0, summed feature 3 (comprising C16 : 1 ω7c and/or C16 : 1 ω6c) and iso-C15 : 0, while the major fatty acids of strain MDT2-14T were anteiso-C15 : 0 and anteiso-C17 : 0. Based on these data, we propose two novel species, Arthrobacter cheniae sp. nov. (Hz2T = CGMCC 1.9262T=NBRC 113086T) and Arthrobacter frigidicola sp. nov. (MDT2-14T=CGMCC 1.9882T=NBRC 113089T).

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“…Strain 785 T contained alanine, glutamic acid, lysine and threonine in the cell-wall peptidoglycan with the A4α type, which was different from A. citreus DSM 20133 T [41], A. koreensis CA15-8 T [42] and A. luteolus CF-25 T [11]. Strain J391 T , representing the second cluster, had a cell wall of the A3α type, with the existence of alanine, glutamic acid, glycine and lysine, which was similar to A. agilis DSM 20550 T [41] and A. bussei KR32 T [8] ( www. peptidoglycan-types.…”

Section: 000mentioning

confidence: 92%

“…The cell-wall peptidoglycan structural type is either A3α or A4α [2,5]. Members of the genus Arthrobacter were isolated from a variety of sources, including animals [6,7], cheese [8], glaciers [9,10], humans [11][12][13], sediments [14] and soil [15]. While investigating the microbiota of the Tibetan Plateau wildlife in PR China, we recently isolated some novel bacterial species from plateau pika (Ochotona curzoniae) and Tibetan wild ass (Equus kiang), including Actinomyces gaoshouyii [16], Neisseria chenwenguii [17], Neisseria weixii [18] and Norcardoides yefusunii [19].…”

mentioning

confidence: 99%

Arthrobacter yangruifuii sp. nov. and Arthrobacter zhaoguopingii sp. nov., two new members of the genus Arthrobacter

Ge

¹

,

Tao

²

,

Yang

³

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Four unknown strains belonging to the genus Arthrobacter were isolated from plateau wildlife on the Qinghai–Tibet Plateau of PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four isolates were separated into two clusters. Cluster I (strains 785T and 208) had the greatest 16S rRNA gene sequence similarity to Arthrobacter citreus (98.6 and 98.7 %, respectively), Arthrobacter luteolus (98.0 and 98.1%, respectively), Arthrobacter gandavensis (97.9 and 98.0 %, respectively) and Arthrobacter koreensis (97.6 and 97.7 %, respectively). Likewise, cluster II (strains J391T and J915) had the highest sequence similarity to Arthrobacter ruber (98.6 and 98.3 %, respectively) and Arthrobacter agilis (98.1 and 97.9 %, respectively). Average nucleotide identity and the digital DNA–DNA hybridization values illustrated that the two type strains, 785T and J391T, represented two separate novel species that are distinct from all currently recognized species in the genus Arthrobacter . These strains had DNA G+C contents of 66.0–66.1 mol% (cluster I) and 68.0 mol% (cluster II). The chemotaxonomic properties of strains 785T and J391T were in line with those of the genus Arthrobacter : anteiso-C15:0 (79.3 and 40.8 %, respectively) as the major cellular fatty acid, MK-8(H2) (65.8 %) or MK-9(H2) (75.6 %) as the predominant respiratory quinone, a polar lipid profile comprising diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipids and phospholipid, and A3α or A4α as the cell wall peptidoglycan type. On the basis of our results, two novel species in the genus Arthrobacter are proposed, namely Arthrobacter yangruifuii sp. nov. (type strain, 785T=CGMCC 1.16725T=GDMCC 1.1592T=JCM 33491T) and Arthrobacter zhaoguopingii sp. nov. (type strain, J391T=CGMCC 1.17382T=GDMCC 1.1667T=JCM 33841T).

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“…G+C content of the genome including plasmids was 64. 4.0 and 5.0), phosphate buffer (pH 6.0-8.0) and glycine buffer (pH 9.0 and 10.0) were used to adjust the pH in NB media, as previously described [37]. Anaerobic growth was tested on R2A agar in an anaerobic jar by using the Anaerocult system (Merck).…”

Section: Genome Featuresmentioning

confidence: 99%

Sphingomonas aliaeris sp. nov., a new species isolated from pork steak packed under modified atmosphere

Heilborn

¹

,

Reinmüller

²

,

Hölzl

³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Species belonging to the genus Sphingomonas have been isolated from environments such as soil, water and plant tissues. Many strains are known for their capability of degrading aromatic molecules and producing extracellular polymers. A Gram-stain-negative, strictly aerobic, motile, red-pigmented, oxidase-negative, catalase-positive, rod-shaped strain, designated DH-S5T, has been isolated from pork steak packed under CO2-enriched modified atmosphere. Cell diameters were 1.5×0.9 µm. Growth optima were at 30 °C and at pH 6.0. Phylogenetic analyses based on both complete 16S rRNA gene sequence and whole-genome sequence data revealed that strain DH-S5T belongs to the genus Sphingomonas , being closely related to Sphingomonas alpina DSM 22537T (97.4 % gene sequence similarity), followed by Sphingomonas qilianensis X1T (97.4 %) and Sphingomonas hylomeconis GZJT-2T (97.3 %). The DNA G+C content was 64.4 mol%. The digital DNA–DNA hybridization value between the isolate strain and S. alpina DSM 22537T was 21.0 % with an average nucleotide identity value of 77.03 %. Strain DH-S5T contained Q-10 as the ubiquinone and major fatty acids were C18 : 1 cis 11 (39.3 %) and C16 : 1 cis 9 (12.5 %), as well as C16 : 0 (12.1 %) and C14 : 0 2-OH (11.4 %). As for polar lipids, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, dimethylphosphatidylethanolamine and sphingoglycolipid could be detected, alongside traces of monomethylphosphatidylethanolamine. Based on its phenotypic, chemotaxonomic and phylogenetic characteristics, strain DH-S5T (=DSM 110829T=LMG 31606T) is classified as a representative of the genus Sphingomonas , for which the name Sphingomonas aliaeris sp. nov. is proposed.

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Arthrobacter bussei sp. nov., a pink-coloured organism isolated from cheese made of cow’s milk

Abstract: A pink-coloured bacterium (strain KR32T) was isolated from cheese and assigned to the ‘ Arthrobacter agilis group’. Members of the ‘pink … Show more

Cited by 20 publications

References 51 publications

Arthrobacter cheniae and Arthrobacter frigidicola sp. nov., isolated from a glacier

Arthrobacter cheniae and Arthrobacter frigidicola sp. nov., isolated from a glacier

Arthrobacter yangruifuii sp. nov. and Arthrobacter zhaoguopingii sp. nov., two new members of the genus Arthrobacter

Sphingomonas aliaeris sp. nov., a new species isolated from pork steak packed under modified atmosphere

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