Arthrobacter cheniae and Arthrobacter frigidicola sp. nov., isolated from a glacier

Yang, Leilei; Liu, Hong‐Can; Liu, Qing; Xin, Yu‐Hua

doi:10.1099/ijsem.0.005177

Cited by 15 publications

(3 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…UMCV2 was initially identified as Arthrobacter agilis since its 16S rRNA sequences showed a percent identity above 99.5% ( Valencia-Cantero et al, 2007 ). However, the 16S rRNA sequences of A. echini ( Lee et al, 2016 ), A. ruber ( Liu et al, 2018 ), A. bussei ( Flegler et al, 2020 ), A. sedimenti ( Lin et al, 2020 ), and A. cheniae ( Yang et al, 2021 ) also share a percent identity above 99.5% with the 16S rRNA gene of Arthrobacter sp. UMCV2.…”

Section: Discussionmentioning

confidence: 99%

Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine

et al. 2022

View full text Add to dashboard Cite

Quorum sensing (QS) is a bacterial cell-cell communication system with genetically regulated mechanisms dependent on cell density. Canonical QS systems in gram-negative bacteria possess an autoinducer synthase (LuxI family) and a transcriptional regulator (LuxR family) that respond to an autoinducer molecule. In Gram-positive bacteria, the LuxR transcriptional regulators “solo” (not associated with a LuxI homolog) may play key roles in intracellular communication. Arthrobacter sp. UMCV2 is an actinobacterium that promotes plant growth by emitting the volatile organic compound N, N-dimethylhexadecylamine (DMHDA). This compound induces iron deficiency, defense responses in plants, and swarming motility in Arthrobacter sp. UMCV2. In this study, the draft genome of this bacterium was assembled and compared with the genomes of type strains of the Arthrobacter genus, finding that it does not belong to any previously described species. Genome explorations also revealed the presence of 16 luxR-related genes, but no luxI homologs were discovered. Eleven of these sequences possess the LuxR characteristic DNA-binding domain with a helix-turn-helix motif and were designated as auto-inducer-related regulators (AirR). Four sequences possessed LuxR analogous domains and were designated as auto-inducer analogous regulators (AiaR). When swarming motility was induced with DMHDA, eight airR genes and two aiaR genes were upregulated. These results indicate that the expression of multiple luxR-related genes is induced in actinobacteria, such as Arthrobacter sp. UMCV2, by the action of the bacterial biocompound DMHDA when QS behavior is produced.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Phylogenetic studies, supported by BLAST analyses of 16S rRNA gene sequences, indicated that the strains R5, ED-ICE-B-R1, and ED-ICE-B-G2 grouped together in the Arthrobacter clade, which was subdivided into two subgroups. One group comprised the red-pigmented R5 and ED-ICE-B-R1, which shared 99.53% of 16S rRNA gene sequence identity and were closely related to the red-pigmented Arthrobacter agilis strain IHBB 9979 [50] and Arthrobacter cheniae strain [51], respectively. The other group comprised the yellow-pigmented ED-ICE-B-G2, which was closely related to the yellow Antarctic Arthrobacter flavus strain IHBB 9551 [52] and shared 100% sequence identity with it (Figure 4).…”

Section: Actinobacteriamentioning

confidence: 99%

Biodiversity of UV-Resistant Bacteria in Antarctic Aquatic Environments

Coppola

Lauritano

Zazo

et al. 2023

JMSE

View full text Add to dashboard Cite

Antarctica is an untapped reservoir of bacterial communities, which are able to adapt to a huge variety of strategies to cope with extreme conditions and, therefore, are capable of producing potentially valuable compounds for biotechnological applications. In this study, 31 UV-resistant bacteria collected from different Antarctic aquatic environments (surface sea waters/ice and shallow lake sediments) were isolated by UV-C assay and subsequently identified. A phylogenetic analysis based on 16S rRNA gene sequence similarities showed that the isolates were affiliated with Proteobacteria, Actinobacteria and Firmicutes phyla, and they were clustered into 15 bacterial genera, 5 of which were Gram negative (Brevundimonas, Qipengyuania, Sphingorhabdus, Sphingobium, and Psychrobacter) and 10 of which were Gram positive (Staphylococcus, Bacillus, Mesobacillus, Kocuria, Gordonia, Rhodococcus, Micrococcus, Arthrobacter, Agrococcus, and Salinibacterium). Strains belonging to Proteobacteria and Actinobacteria phyla were the most abundant species in all environments. The genus Psychrobacter was dominant in all collection sites, whereas bacteria belonging to Actinobacteria appeared to be the most diverse and rich in terms of species among the investigated sites. Many of these isolates (20 of 31 isolates) were pigmented. Bacterial pigments, which are generally carotenoid-type compounds, are often involved in the protection of cells against the negative effects of UV radiation. For this reason, these pigments may help bacteria to successfully tolerate Antarctic extreme conditions of low temperature and harmful levels of UV radiation.

show abstract

“…In the last decade, we isolated thousands of heterotrophic bacterial strains from the surface samples of glaciers in Western China, including some common taxa in the cryosphere, such as Arthrobacter, Cryobacterium, Flavobacterium, Polaromonas, Sphingomonas , and Janthinobacterium (Liu et al, 2015 ). Studies investigating these strains have promoted not only the discovery and validation of new species (Liu et al, 2019a , 2020b , 2021b ; Zhang et al, 2020 ; Yang et al, 2021 ) but also the understanding of novel functions (Liu et al, 2021a ) and evolutionary characteristics (Liu et al, 2020a ) of cold-adapted bacteria.…”

Section: Introductionmentioning

confidence: 99%

Diversity of the genus Cryobacterium and proposal of 19 novel species isolated from glaciers

2023

Self Cite

View full text Add to dashboard Cite

The bacterial genus Cryobacterium includes at present 14 species that live in cryospheric environments. In this study, we analyzed 101 genomes of Cryobacterium with pure cultures obtained from GenBank. They could be classified into 44 species based on average nucleotide identity (ANI) analysis, showing the diversity of Cryobacterium. Among these, 19 strains in our laboratory were isolated from the glacier samples in China. The pairwise ANI values of these 19 strains and known species were <95%, indicating that they represented 19 novel species. The comparative genomic analysis showed significant differences in gene content between the two groups with a maximum growth temperature (Tmax) of ≤ 20°C and a Tmax of >20°C. A comprehensive and robust phylogenetic tree, including 14 known species and 19 novel species, was constructed and showed five phylogenetic branches based on 265 concatenated single-copy gene sequences. The Tmax parameter had a strong phylogenetic signal, indicating that the temperature adaptation of Cryobacterium was largely through vertical transfer rather than horizontal gene transfer and was affected by selection. Furthermore, using polyphasic taxonomy combined with phylogenomic analysis, we proposed 19 novel species of the genus Cryobacterium by the following 19 names: Cryobacterium serini sp. nov., Cryobacterium lactosi sp. nov., Cryobacterium gelidum sp. nov., Cryobacterium suzukii sp. nov., Cryobacterium fucosi sp. nov., Cryobacterium frigoriphilum sp. nov., Cryobacterium cryoconiti sp. nov., Cryobacterium lyxosi sp. nov., Cryobacterium sinapicolor sp. nov., Cryobacterium sandaracinum sp. nov., Cryobacterium cheniae sp. nov., Cryobacterium shii sp. nov., Cryobacterium glucosi sp. nov., Cryobacterium algoritolerans sp. nov., Cryobacterium mannosilyticum sp. nov., Cryobacterium adonitolivorans sp. nov., Cryobacterium algoricola sp. nov., Cryobacterium tagatosivorans sp. nov., and Cryobacterium glaciale sp. nov. Overall, the taxonomy and genomic analysis can improve our knowledge of phenotypic diversity, genetic diversity, and evolutionary characteristics of Cryobacterium.

show abstract

Arthrobacter cheniae and Arthrobacter frigidicola sp. nov., isolated from a glacier

Cited by 15 publications

References 24 publications

Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine

Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine

Biodiversity of UV-Resistant Bacteria in Antarctic Aquatic Environments

Diversity of the genus Cryobacterium and proposal of 19 novel species isolated from glaciers

Contact Info

Product

Resources

About