Artemisinin relieves myocardial ischemia-reperfusion injury via modulating miR-29b-3p and hemicentin 1

Abstract

Objective: To explore the impact of artemisinin (ARS) on myocardial ischemia-reperfusion (I/R) injury and the underlying mechanism.Methods: Myocardial I/R rat model and cell model were used in this study. The cell viability, morphological changes, apoptosis, and oxidative stress were evaluated in cardiomyocytes H9c2 cells in vitro by using cell counting kit-8, microscope, flow cytometry, and commercial kits. High throughput sequencing is used to identify molecular targets of ARS on myocardial I/R injury, and then the gene-gene interaction network was constructed. MiR-29b-3p, hemicentin 1 (HMCN1), and apoptosis-related genes were tested by qRT-PCR and Western blotting. In the myocardial I/R rat model, echocardiography, (Triphenyl tetrazolium chloride) TTC staining, Hematoxylin-eosin (H&E) staining, Masson Trichrome staining, and TUNEL staining are applied to evaluate the protective effect of ARS on the myocardial injury.Results:In vitro, we demonstrated that ARS alleviated H2O2-induced myocardial I/R injury, manifested by increased H9c2 viability, decreased pathological changes, apoptosis, and oxidative stress biomarker ROS, LDH, and CK-MB. Then, sequencing analysis revealed that miR-29b-3p/HMCN1 was the target of ARS for myocardial I/R injury. Notably, rescue experiments indicated that ARS inhibited myocardial I/R injury through targeted regulation miR-29b-3p/HMCN1. In vivo, we confirmed that ARS reduced myocardial injury, fibrosis, and apoptosis via modulation of miR-29b-3p/HMCN1.Conclusion: This study demonstrated the functional role of the ARS/miR-29b-3p/HMCN1 axis in alleviating myocardial I/R injury, which provided a new direction for myocardial I/R injury therapy.

Untargeted metabolomics revealed the regulatory effect of Maillard reaction products of ovalbumin with different degrees on the metabolism of colitis mice

“…This is similar to the previous study. 19 When aging mice were treated with MRPs of rapeseed peptide, the antioxidant enzyme in mice increased and may inhibit the NF-κB pathway and showed anti-inflammatory activity in the aging model treated with d -galactose. 30 DAO is an intracellular enzyme in intestinal epithelial cells, which can be released into the blood when the intestinal mucosal epithelium is damaged.…”

“…Masson's staining was performed according to ref. 19 with some modifications. Paraffin sections are dewaxed to water.…”

Evaluating the pro-survival potential of apoptotic bodies derived from 2D- and 3D- cultured adipose stem cells in ischaemic flaps

“…8E). The functions of these miRNAs have been documented in the literature [45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61]. Among them, the top five with the most notable expression in upregulation and downregulation havd been shown to contribute to the promotion of ischaemic flap survival (Fig.…”

Irisin ameliorates myocardial ischemia-reperfusion injury by modulating gut microbiota and intestinal permeability in rats

“…Acute myocardial infarction (AMI) is a common cardiac emergency associated with high rates of morbidity and mortality [1]. For patients with ST-elevation MI (STEMI), thrombolytic/ fibrinolytic therapy or percutaneous coronary intervention (PCI) is considered the effective reperfusion strategy that should be performed [2]. Because reperfusion can induce excessive production of reactive oxygen species, excessive inflammatory response and cell apoptosis, the process is known as myocardial ischemia-reperfusion (I/R) injury [3].…”