Arsenicicoccus piscis sp. nov., a Mesophilic Actinobacterium Isolated from the Intestinal Tract of a Fish

Abstract

A Gram-positive bacterium, designated strain Kis4-19 T , was isolated from the intestinal tract of a fish, and its taxonomic position was investigated by a polyphasic approach. The cells of strain Kis4-19 T were coccus-shaped, non-motile and non-sporulating. The peptidoglycan type of this organism was A3; LL-diaminopimeric acid (LL-A 2 pm) was the diagnostic diamino acid of the peptidoglycan. The predominant menaquinone was MK-8(H 4 ), and the major fatty acids were iso-C 15:0 , iso-C 14:0 and iso-C 16:0 . Galactose was detected as a cell-wall sugar. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and lyso-phosphatidylethanolamine. The DNA G+C content was 71.1 mol%. Sequencing analysis of the 16S rRNA gene indicated that strain

Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.

“…Two actinobacteria strains, Aji5-31 T and Ngc37-23 T , were isolated from the intestinal tracts of two species of fishes, Trachurus japonicus and Repomucenus richardsonii, respectively, which were collected from Kyonan Beach on the coast of Tokyo Bay, Japan. The procedure employed for isolating the bacteria from the intestinal tracts was described by Hamada et al (2009). NBRC medium 802 (1.0% Polypepton (Wako), 0.2% yeast extract (Difco), 0.1% MgSO 4 7H 2 O and 1.5% agar if required; pH 7.0) was used for general laboratory cultivation, morphological study and determination of optimal growth parameters.…”

Description of Herbiconiux flava sp. nov. and emended description of the genus Herbiconiux

“…Cell walls were separated from unbroken cells by differential centrifugation in distilled water and further purified in boiling 4 % SDS (100 u C, 30 min), followed by several washings with distilled water. The molar ratio of amino acids in cell-wall hydrolysates (4 M HCl, 16 h) were determined using the method described by Hamada et al (2009). Amino-acid isomers in cell-wall hydrolysates were examined using the method described by Nozawa et al (2007) using liquid chromatography MS (model LCMS-2020; Shimadzu).…”

“…Growth under anaerobic conditions was determined by incubation in an anaerobic chamber with an O 2 -absorbing and CO 2 -generating agent (Anaero-Pack; Mitubishi Gas Chemical). Cell motility and growth at different pH and with different NaCl concentrations were determined using liquid basal medium, as outlined by Hamada et al (2009). Growth at 5-25 u C (at intervals of 5 u C), 28, 37 and 45 u C was determined on solid basal medium after 3 days and growth at 5 and 10 u C was also evaluated after 10 days.…”

“…PCR amplification and sequencing of the 16S rRNA gene of strain NBRC 16403 T was performed as described previously (Hamada et al, 2009). Phylogenetic neighbours were identified and pairwise 16S rRNA gene sequence similarities were calculated using the EzTaxon server (http://www.…”

“…for the second direction. Isoprenoid quinones, cell-wall sugars and DNA G+C content were analysed according to the methods outlined by Hamada et al (2009).…”