Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.

Hamada, Moriyuki; Iino, Takao; Iwami, Takahiro; Harayama, Shigeaki; Tamura, Tomohiko; Suzuki, Ken‐ichiro

doi:10.2323/jgam.56.427

Cited by 78 publications

(45 citation statements)

References 24 publications

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“…29 Isoprenoid quinones and polar lipids were extracted and isolated using standard procedures, 30 and the results were compared with those of appropriate controls. The isoprenoid quinone content was determined using liquid chromatography/mass spectrometry described by Hamada et al 31 The polar lipids were identified by two dimensional thin-layered chromatography, followed by spraying with appropriate detection reagents according to the method of Yassin et al 32 The DNA G+C content of strain RI50-RCA114 T was determined by HPLC as described by Tamaoka and Komagata 33…”

Section: Phenotypic Characterizationmentioning

confidence: 99%

Actinoplanes rishiriensis sp. nov., a novel motile actinomycete isolated by rehydration and centrifugation method

et al. 2012

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The motile actinomycete strain RI50-RCA114 T was isolated using rehydration and centrifugation method from a soil sample obtained from Rishiri Island in Japan. The taxonomic status of this organism was established using a polyphasic approach. Cells of strain RI50-RCA114 T were Gram positive, aerobic, motile and formed irregular sporangia. The strain grew in the presence of 0-2% (w/v) NaCl, between pH 5 and 8, and over a temperature range of 20-371C, with optimal growth at 301C. Whole-cell hydrolysates of the strain contained meso-diaminopimelic acid, galactose, glucose and mannose, in addition to one unidentified O-methyl-hexose. The predominant menaquinone was MK-9(H 4 ), and the major polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol and phosphatidyl-N-methylethylethanolamine. The major cellular fatty acids were iso-C 16:0 , iso-C 15:0 and anteiso-C 17:0 . Comparative 16S ribosomal RNA gene sequence analysis revealed that strain RI50-RCA114 T had the closest sequence similarity with Actinoplanes globisporus JCM 3186 T (97.6%). However, DNA-DNA hybridization assays as well as physiological and biochemical analyses differentiated strain RI50-RCA114 T from its closest phylogenetic relative. On the basis of these data, we propose that strain RI50-RCA114 T (¼NBRC 108556 T ¼BCC 49184 T ) be classified as the type strain of a novel Actinoplanes species and named Actinoplanes rishiriensis sp. nov.

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Section: Phenotypic Characterizationmentioning

confidence: 99%

Actinoplanes rishiriensis sp. nov., a novel motile actinomycete isolated by rehydration and centrifugation method

et al. 2012

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“…The morphological characteristics of strain OAct353 T were observed for up to 7 days using light microscopy (model BH 2; Olympus) and scanning electron microscopy (model JSM-840; JEOL). Cell motility and Gram-staining were determined using the methods described by Hamada et al (2010). Growth under anaerobic and microaerobic conditions was determined using AnaeroPack-Anaero and AnaeroPack-MicroAero (Mitsubishi Gas Chemical) with anaerobic jars.…”

mentioning

confidence: 99%

Agromyces binzhouensis sp. nov., an actinobacterium isolated from a coastal wetland of the Yellow River Delta

Chen

Guan

Wang

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive, heterotrophic, non-spore-forming, rod-shaped strain, designated OAct353 T , belonging to the genus Agromyces was isolated from a soil sample collected from a coastal wetland of the Yellow River delta, PR China. The strain was identified using a polyphasic taxonomic approach. The strain grew in the presence of 0-10 % (w/v) NaCl (optimum 2-3 %), at pH 5.0-8.0 (optimum pH 7.0) and 12-36 C (optimum 28 C). The isolate contained 2,4-diaminobutyric acid, glutamic acid and glycine in its peptidoglycan. The acyl type of the cell-wall muramic acid was N-acetyl. The whole-cell sugars of this novel strain were glucose, xylose and rhamnose. The predominant menaquinones were MK-12 (74 %) and MK-11 (21 %). The major phospholipids were phosphatidylglycerol, one unknown phospholipid, three unknown glycolipids and three unknown polar lipids. The major fatty acids were iso-C 16:0 , anteiso-C 15:0 and anteiso-C 17:0 . The DNA G+C content was 69.6 mol %. DNA-DNA relatedness clearly separated strain OAct353 T from its closest relatives. On the basis of phenotypic, phylogenetic and chemotaxonomic data, a novel species, Agromyces binzhouensis sp. nov., is proposed. The type strain is OAct353 T (=CGMCC4.7180 T =DSM 28305 T =NRRL B-59115 T ).

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“…T were extracted and examined by one-and two-dimensional TLC by using a previously described method (Hamada et al, 2010), and the patterns were visualized by spraying the TLC plates with 5 % phosphomolybdic acid, Dittmer-Lester reagent (Dittmer & Lester, 1964), Schiff reagent and anisaldehyde. In the two-dimensional TLC analysis, the first and second solvents were chloroform/methanol/water (65 : 25 : 4, by volume) and chloroform/acetic acid/methanol/water (80 : 18 : 12 : 5, by volume), respectively, and the polar lipids of strain 2a_47_2 T and 2 reference strains (Haloterrigena daqingensis NBRC 105739 T and Haloarcula japonica NBRC 101032 T ) were developed ( Fig.…”

mentioning

confidence: 99%

Halobium palmae gen. nov., sp. nov., an extremely halophilic archaeon isolated from a solar saltern

Mori

Nurcahyanto

Kawasaki

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Mobilicoccus pelagius gen. nov., sp. nov. and Piscicoccus intestinalis gen. nov., sp. nov., two new members of the family Dermatophilaceae, and reclassification of Dermatophilus chelonae (Masters et al. 1995) as Austwickia chelonae gen. nov., comb. nov.

Cited by 78 publications

References 24 publications

Actinoplanes rishiriensis sp. nov., a novel motile actinomycete isolated by rehydration and centrifugation method

Actinoplanes rishiriensis sp. nov., a novel motile actinomycete isolated by rehydration and centrifugation method

Agromyces binzhouensis sp. nov., an actinobacterium isolated from a coastal wetland of the Yellow River Delta

Halobium palmae gen. nov., sp. nov., an extremely halophilic archaeon isolated from a solar saltern

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