Agromyces binzhouensis sp. nov., an actinobacterium isolated from a coastal wetland of the Yellow River Delta

Abstract

A Gram-stain-positive, heterotrophic, non-spore-forming, rod-shaped strain, designated OAct353 T , belonging to the genus Agromyces was isolated from a soil sample collected from a coastal wetland of the Yellow River delta, PR China. The strain was identified using a polyphasic taxonomic approach. The strain grew in the presence of 0-10 % (w/v) NaCl (optimum 2-3 %), at pH 5.0-8.0 (optimum pH 7.0) and 12-36 C (optimum 28 C). The isolate contained 2,4-diaminobutyric acid, glutamic acid and glycine in its peptidoglycan. The acyl type of the cell-wall muramic acid was N-acetyl. The whole-cell sugars of this novel strain were glucose, xylose and rhamnose. The predominant menaquinones were MK-12 (74 %) and MK-11 (21 %). The major phospholipids were phosphatidylglycerol, one unknown phospholipid, three unknown glycolipids and three unknown polar lipids. The major fatty acids were iso-C 16:0 , anteiso-C 15:0 and anteiso-C 17:0 . The DNA G+C content was 69.6 mol %. DNA-DNA relatedness clearly separated strain OAct353 T from its closest relatives. On the basis of phenotypic, phylogenetic and chemotaxonomic data, a novel species, Agromyces binzhouensis sp. nov., is proposed. The type strain is OAct353 T (=CGMCC4.7180 T =DSM 28305 T =NRRL B-59115 T ).

Agromyces badenianii sp. nov., isolated from plateau pika (Ochotona curzoniae)

“…atrinae DSM 23870 T , A. subbeticus JCM 14324 T and other members of the genus Agromyces [1–10], strains MF30-A T and MF845 had anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 as their major fatty acids (), supporting the affiliation of the two strains to the genus Agromyces . However, strains MF30-A T and MF845 had more anteiso-C 15 : 0 (52.6 and 52.4 %, respectively) than A.…”

“…subbeticus JCM 14324 T (MK-12 : MK-13, 58 : 35) [8]. MK-12 was a predominant menaquinone in MF30-A T , further suggesting the affiliation of our strains to the genus of Agromyces [1–10, 23]. However, the proportion of MK-12 in strain MF30-A T was higher than that detected in the other three strains.…”

“…The genus Agromyces was created in 1969 with Agromyces ramosus isolated from soil as the type species [1], and amended in 1992 [2]. At the time of writing, an authoritative database (www.bacterio.net) shows that the genus contains 31 known species and two subspecies with most of them isolated from soil [1, 3–5] or soil-associated environments [6–11]. Herein, we propose a novel species of the genus Agromyces represented by strains MF30-A T and MF845, which were isolated from the intestinal contents of plateau pika ( Ochotona curzoniae ) from the Qinghai–Tibet Plateau, PR China.…”

Agromyces kandeliae sp. nov., isolated from rhizosphere soil of Kandelia candel in a mangrove

“…nov. is proposed. *Data from Chen et al [43] and 2,4-diaminobutyric acid. The whole-cell sugars are rhamnose, glucose and galactose.…”

Agromyces laixinhei sp. nov. isolated from bat feces in China

“…The genus Agromyces was first proposed in 1969 (Gledhill and Casida, 1969), and amended later in 1992 and 2004, respectively (Zgurskaya et al, 1992;Ortiz-Martinez et al, 2004). Members of the genus Agromyces are aerobic, non-motile and Gram-stain-positive, with MK-11 and MK-12 as the predominant menaquinones, and anteiso-C 15:0 , anteiso-C 17:0 , and iso-C 16:0 as the primary fatty acids (Chen et al, 2016;Corretto et al, 2016;Huang et al, 2016). As of the end of 2020, the genus is comprised of 36 species and two subspecies (http://www.bacterio.net/agromyces.html) with valid published names (Parte, 2018), mostly isolated from various environmental sources such as soil (Chen et al, 2016;Huang et al, 2016), the rhizosphere (Corretto et al, 2016), caves and rocks, plant tissues, sea sediments, and catacombs.…”