Description of Herbiconiux flava sp. nov. and emended description of the genus Herbiconiux

Abstract

D-alanine, glycine and threo-3-hydroxyglutamic acid, which replaced glutamic acid almost completely. The predominant menaquinones were MK-10 and MK-11. The polar lipid pattern comprised diphosphatidylglycerol, phosphatidylglycerol, three glycolipids and minor amounts of other polar lipids. The major fatty acids were anteiso-C 15 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 ; no cyclohexyl-C 17 : 0 was detected. The DNA G+C content was 71.0 mol%. The results of phylogenetic and DNA-DNA relatedness studies, along with phenotypic differences between strain NBRC 16403 T and recognized members of the genus Herbiconiux, indicated that the isolate should be assigned to a novel species of the genus Herbiconiux, for which the name Herbiconiux flava sp. nov. is proposed. The type strain is NBRC 16403 T (5VKM Ac-2058 T ). Kim et al., 2012), isolated from the gut of hairy longhorned toad beetles. During the course of quality control of the collections of the NITE Biological Resource Center (NBRC), it was revealed that strain NBRC 16403 T , which was labelled as Curtobacterium sp., was phylogenetically related to members of the genus Herbiconiux. The genusStrain NBRC 16403 T (originally designated DL 374 T ) was isolated from the phyllosphere of Carex sp. collected in the Central Chernozem Nature Park (Belgorod Region, Russia) on Corynebacterium agar (DSMZ medium 53: containing 1.0 % casein peptone tryptic digest, 0.5 % yeast extract, 0.5 % glucose, 0.5 % NaCl and 1.5 % agar; pH 7.2) after incubation at room temperature (18-24 u C) in daylight for 1 month, as described by Dorofeeva et al. (2002). The strain was Gram-stain-positive and the plate from which it was isolated bore Gram-negative colonies in the majority. NBRC medium 802 [1.0 % polypeptone (Wako), 0.2 % yeast extract (Difco), 0.1 % MgSO 4 . 7H 2 O; pH 7.0; 1.5 % agar if required] was used as the basal medium for this study. Biomass for chemotaxonomic and molecular systematic studies (except for polar lipid analysis) was obtained by incubating the isolate in shake flasks (100 r.p.m.) for 48 h at 28 u C.Colony appearance was examined after incubation at 28 u C for 3 days. Cell morphology was investigated by examining cells from cultures over a whole growth cycle (up to 5 days) under a light microscope (BX-51; Olympus) and a scanning electron microscope (JSM-6060; JEOL). Growth Abbreviations: DAB, 2,4-diaminobutyric acid; Hyg, threo-3-hydroxyglutamic acid.

Exploring gastric bacterial community in young pigs

“…Instead, the bacterial genera which characterise the gastric mucosa were Herbiconiux and Brevundimonas ; Herbiconiux included strains associated with plant matter [ 29 , 30 ] which has never been described before in the gastrointestinal tract of mammals. The genus Brevundimonas includes opportunistic strains ubiquitous in the environment, and a strain with growth requirements typical of Helicobacter wich has been reported in the gastric mucosa of dogs [ 31 ].…”