Armazenamento de sementes e concentrações de ágar no cultivo in vitro de Brassavola tuberculata Hook. (Orchidaceae)

Sousa, Gisele Garcia de; Otubo, Benedita Maria Rodrigues; Sorgato, José Carlos; Soares, Jackeline Schultz; Ribeiro, Luan Marlon

doi:10.21826/2446-82312020v75e2020017

Cited by 2 publications

(2 citation statements)

References 14 publications

(19 reference statements)

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“…Despite the widespread use of in vitro cultivation techniques for orchids, the in vitro propagation of Brassavola species still lacks established protocols, primarily due to the limited number of studies focused on these species (Pereira et al, 2022;Xu et al, 2022;Vendrame et al, 2023). Regarding B. tuberculata, only a few scientific articles have been published on its in vitro germination (Herrmann et al, 2011;Soares et al, 2012Soares et al, , 2020Rosa et al, 2013), seed storage and viability (Macedo et al, 2014;Soares et al, 2014;Sousa et al, 2020), multiplication and in vitro rooting (Mengarda et al, 2017), and ex vitro acclimatization (Sousa et al, 2015).…”

Section: In Vitro Growthmentioning

confidence: 99%

Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose

et al. 2023

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This study examines the in vitro growth and ex vitro establishment of Brassavola tuberculata in relation to the micropropagation system and sucrose concentration employed in the in vitro culture. A completely randomized experimental design was utilized, employing a 2 x 5 factorial arrangement. The experimental period began with seedlings cultivated in vitro for 180 days, which were subsequently transferred to Murashige and Skoog culture media containing sucrose concentrations of 0, 15, 30, 45, or 60 g L-1. The cultures were subjected to two micropropagation systems: conventional and gas exchange. After 90 days of in vitro cultivation, the plants were evaluated, transplanted into a substrate, and placed in a screened nursery for ex vitro cultivation. After 300 days of ex vitro cultivation, the survival and initial characteristics of the plants were assessed. The micropropagation system allowing gas exchange and sucrose concentrations up to 30 g L-1 enhanced the shoot and root growth of in vitro propagated plants. No noticeable anatomical differences were observed after 90 days of in vitro culture among the different sucrose concentrations and micropropagation systems used. In the ex vitro establishment, irrespective of sucrose concentration, the micropropagation system facilitating gas exchange positively influenced all evaluated characteristics.

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Section: In Vitro Growthmentioning

confidence: 99%

Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose

et al. 2023

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“…Germination responses may vary within the same family, genus, or even species. These responses depend on the conditions in which the fruits are produced, their maturation stage, and the processes used in storage (Juras et al, 2019;Sousa et al, 2020;Ferreira et al, 2022) (Figure 1), shows that, in addition to presenting high %G, the treatment with W light and SC sealing (W light -SC) was the only cultivation condition that led to root formation at 60 days after sowing.…”

Section: Germination At 30 and 60 Days After Sowingmentioning

confidence: 99%

Light condition, flask sealing, and cultivation time on the germination and initial in vitro development of Dendrobium nobile Lindl.

Ribeiro

Soares

et al. 2022

Ornam. Hortic.

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One of the obstacles for the production of Orchidaceae plants is the seed propagation. Thus, in in vitro cultivation, luminosity and the type of sealing of the bottles are factors that influence germination and initial development of orchids. Therefore, the objective was to evaluate the light condition, the type of sealing of the bottles and the evaluation time in germination and initial in vitro development of Dendrobium nobile Lindl. After sowing, cultures were subjected to two irradiance conditions according to the sealing of the flasks: 7.0 µmol m-2 s-1 (threaded cap) and 19.0 µmol m-2 s-1 (plastic film) and two light conditions: white fluorescent lamp and red fluorescent lamp. At 30, 60 and 90 days, the percentage of germination, survival, of protocorms in stage 1, stage 2, stage 3 and stage were evaluated 4. DIC was used, in a sub-divided plot scheme for 30 and 60 days after sowing and in a 2 x 2 factorial scheme at 90 days. The highest germination percentage was observed when using white fluorescent lamp with a plastic film cover (70.33%) and at 30 days (70.13%). The highest survival percentage (100%) was observed when using plastic film cover and white fluorescent lamp at 90 days. The greatest development of propagules, reaching stage 4 was verified in the red fluorescent lamp with threaded cap (9.55%).

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Armazenamento de sementes e concentrações de ágar no cultivo in vitro de Brassavola tuberculata Hook. (Orchidaceae)

Abstract: Armazenamento de sementes e concentrações de ágar no cultivo in vitro de Brassavola tuberculata Hook. (Orchidaceae)

Cited by 2 publications

References 14 publications

Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose

Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose

Light condition, flask sealing, and cultivation time on the germination and initial in vitro development of Dendrobium nobile Lindl.

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