Light condition, flask sealing, and cultivation time on the germination and initial in vitro development of Dendrobium nobile Lindl.

Ribeiro, Isabella de Souza; Ribeiro, Luan Marlon; Soares, Jackeline Schultz; Ramos, Jéssica Celeste Mônico; Sorgato, José Carlos

doi:10.1590/2447-536x.v28i4.2515

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…In the photomixotrophic system with a lower gas exchange rate (or without natural ventilation), the microenvironment of the flask is the factor with the greatest influence on the growth and development, since carbon is made available through the supply of sucrose in the medium [33][34][35]. Undeniably, sealing the bottles (rigid lids or without membranes) provides high humidity inside them, greater accumulation of the phytohormone ethylene, reduced gas exchange, and low CO 2 concentration [36][37][38][39].…”

Section: Discussionmentioning

confidence: 99%

Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization

Silva,

Soares,

Silva

et al. 2024

Horticulturae

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Eryngium foetidum L., a biennial herb with diverse applications in food and traditional medicine, holds economic and pharmacological significance. Given its growing commercial interest, implementing biotechnological approaches like plant tissue culture is vital for sustainable propagation and metabolite production. In this study, we investigated the in vitro photoautotrophic potential of Eryngium foetidum, examining growth, chlorophyll a fluorescence, photosynthetic pigments, and anatomical features under sucrose concentrations (0 and 30 g L−1) and gas exchange rate (14 and 25 μL L−1 s−1 CO2). Acclimatization and survival rates of plants after ex vitro transfer were also assessed. Eryngium foetidum exhibited robust growth in both photoautotrophic and photomixotrophic conditions, with natural ventilation significantly enhancing plant development. Chlorophyll a fluorescence and photosynthetic performance were influenced by sucrose and gas exchange, highlighting the importance of these factors in plant micropropagation. Moreover, the species demonstrated remarkable plasticity during acclimatization, with high survival rates and rapid inflorescence development. The research provides valuable insights into optimizing in vitro cultivation conditions for Eryngium foetidum, emphasizing the potential for large-scale clonal propagation and exploring secondary metabolites. The observed phenotypic plasticity underscores the adaptability of the species to diverse environments. These biotechnological strategies open avenues for future studies, including the application of elicitors for enhanced secondary metabolite production.

show abstract

Section: Discussionmentioning

confidence: 99%

Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization

Silva,

Soares,

Silva

et al. 2024

Horticulturae

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Impact of monochromatic lights on the in vitro development of Cattleya walkeriana and effects on acclimatization

et al. 2023

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Light quality is an important factor for the adequacy of plant production through plant tissue culture, as it directly interferes with morphogenesis and photosynthetic capacity of explants. The objective of this study was to analyze the impact of monochromatic lights such as light emitting diode (LED) on the in vitro development of Cattleya walkeriana G. and their effects on acclimatization. The plants were developed in vitro under the colors of green, blue, yellow, red, 2 red:1 blue, and white LED lights. For in vitro cultivation, Knudson medium was used, supplemented with 20 mg L-1 sucrose, 5.5 mg L-1 agar, 2% activated charcoal, 100 mL coconut water, and pH 6.0. For ex vitro cultivation, the plants were acclimatized in styrofoam trays containing sphagnum as a substrate. In general, the supplied light lengths impactedin vitro growth and acclimatization analyses. There was influence on the cuticle thickness of plants in vitro. Chlorophyll and carotenoid contents were not significant. We can conclude that light lengths formed by 2 red:1 blue, red and yellow LEDs can be indicated for better performance in the production of C. walkeriana. The 2 red:1 blue and red LEDs provide superior in vitro development than the others, with gains for the species in acclimatization. The yellow LED provided a possible in vitro hardening, which ensured the greatest success of the seedlings during acclimatization.

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Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization

Silva,

Soares,

Silva

et al. 2023

Preprint

View full text Add to dashboard Cite

Eryngium foetidum L., a biennial herb with diverse applications in food and traditional medicine, holds economic and pharmacological significance. Given its growing commercial interest, implementing biotechnological approaches like plant tissue culture is vital for sustainable propagation and metabolite production. In this study, we investigated the in vitro photoautotrophic potential of E. foetidum, examining growth, chlorophyll a fluorescence, photosynthetic pigments, and anatomical features under different sucrose concentrations and gas exchange rates. Acclimatization and survival rates of plants after ex vitro transfer were also assessed. E. foetidum exhibited robust growth in both photoautotrophic and photomixotrophic conditions, with natural ventilation significantly enhancing plant development. Chlorophyll a fluorescence and photosynthetic performance were influenced by sucrose and gas exchange, highlighting the importance of these factors in plant micropropagation. Moreover, the species demonstrated remarkable plasticity during acclimatization, with high survival rates and rapid inflorescence emission. The research provides valuable insights into optimizing in vitro cultivation conditions for E. foetidum, emphasizing the potential for large-scale clonal propagation and exploring secondary metabolites. The observed phenotypic plasticity underscores the adaptability of the species to diverse environments. These biotechnological strategies open avenues for future studies, including the application of elicitors for enhanced secondary metabolite production.

show abstract

Light condition, flask sealing, and cultivation time on the germination and initial in vitro development of Dendrobium nobile Lindl.

Cited by 3 publications

References 29 publications

Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization

Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization

Impact of monochromatic lights on the in vitro development of Cattleya walkeriana and effects on acclimatization

Unlocking the Potential of In Vitro Photoautotrophy for Eryngium foetidum: Biomass, Morphophysiology, and Acclimatization

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