Arecanut (Areca catechu L.) seed polyphenol improves osteoporosis via gut-serotonin mediated Wnt/β-catenin pathway in ovariectomized rats

Meng, Keke; Mei, Fengfeng; Zhu, Lehui; Xiang, Qingying; Quan, Zhangyan; Pan, Feibing; Xia, Guanghua; Shen, Xuanri; Yun, Yong‐Huan; Zhang, Chenghui; Zhong, Qiuping; Chen, Haiming

doi:10.1016/j.jff.2021.104598

Cited by 20 publications

(15 citation statements)

References 69 publications

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“…Rats of Sham and OVX groups were orally administered normal saline, rats in group Tn-L were orally administered 200 mg/kg.bw THLs, those in group Tn-H were orally administered 400 mg/kg.bw THLs, and rats in group E2 were orally administered 300 mg/kg.bw estradiol tablets. After intragastric administration for 90 days, 0.25 mL/100 g.bw 10% chloral hydrate was intraperitoneally injected, and the rats were sacrificed ( 37 , 38 ).…”

Section: Methodsmentioning

confidence: 99%

Tilapia nilotica Head Lipids Improved Bone Loss by Regulating Inflammation and Serum Metabolism Through Gut Microbiota in Ovariectomized Rats

et al. 2022

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Osteoporosis is a global health problem, and it is of great significance to replace the drugs with natural functional factors. In this study, we investigated the antiosteoporotic activity of lipids prepared from Tilapia nilotica fish head lipids (THLs) in the ovariectomized osteoporosis rats. THLs are composed of neutral lipids (NL, 77.84%), phospholipids (PL, 11.86%), and glycolipids (GL, 6.47%). There were apparent differences in the fatty acid composition of disparate components, and PL contains the most abundant Ω-3 polyunsaturated fatty acids. The results proved that THLs could improve bone microstructure, increase bone mineral density, and decrease bone resorption. To illustrate the antiosteoporotic mechanism, we analyzed the changes in gut microbial communities, proinflammation factors, serum metabolites, and metabolic pathways. Further study on gut microbiota showed that THLs significantly decreased the content of Alistipes in the gut and dramatically increased the beneficial bacteria such as Oscillospira, Roseburia, and Dubosiella. Meanwhile, proinflammation factors of serum in OVX rats decreased significantly, and metabolites were changed. Therefore, we speculated that THLs improved bone loss through reducing inflammation and changing the metabolites and metabolic pathways such as arachidonic acid metabolism and primary bile acid metabolism, etc., by altering gut microbiota. The results indicated that THLs could be a functional factor with antiosteoporotic activity.

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Section: Methodsmentioning

confidence: 99%

Tilapia nilotica Head Lipids Improved Bone Loss by Regulating Inflammation and Serum Metabolism Through Gut Microbiota in Ovariectomized Rats

et al. 2022

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“…Meanwhile, the Sham group were intragastric 1 ml/100 g.bw physiological saline. The rats were received allocated administration once a day on the whole experiment, were anesthetized with 10% chloral hydras, and sacrificed by cervical dislocation ( 18 ).…”

Section: Methodsmentioning

confidence: 99%

“…Then, the powder was put into a 2-ml EP tube and extracted total RNA from the bone in accordance with the instructions of the UNIQ-10 column animal total RNA extraction kit. The concentration and purity of total RNA (A260/A280 ratio) in bone tissue samples were determined by an ultra-micro nucleic acid analyzer ( 18 , 21 ).…”

Section: Methodsmentioning

confidence: 99%

Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism

et al. 2022

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In recent years, the development of safe and effective anti-osteoporosis factors has attracted extensive attention. In this study, an estrogen-deficient osteoporosis rat model was employed to study the improving mechanism of sialoglycoprotein isolated from Gadus morhua eggs (Gds) against osteoporosis. The results showed that compared with OVX, Gds ameliorated the trabecular microstructure, especially the increased trabecular thickness, decreased trabecular separation, and enhanced the trabecular number. The analysis of qRT-PCR and western blotting found that Gds reduced bone resorption by inhibiting RANKL-induced osteoclastogenesis. The LC-MS/MS was used to investigate serum metabolism, and the enrichment metabolites were analyzed by the KEGG pathway. The results revealed that the Gds significantly altered the fat anabolism pathway, which includes ovarian steroidogenesis pathway and arachidonic acid metabolism pathway. Altogether, Gds could improve osteoporosis by suppressing high bone turnover via controlling OPG/RANKL/TRAF6 pathway, which is implicated with ovarian steroidogenesis pathway and arachidonic acid metabolism pathway. These findings indicated that Gds could be a candidate factor for anti-osteoporosis.

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“…The mRNA expressions of genes such as AQP1, AQP2, and AQP3 that regulate kidney tissue function and urine formation were detected by RT-PCR. The procedure of RT-PCR was performed according to our previous work ( 19 , 20 ). Briefly, after the kidneys were ground and homogenized, total RNA was extracted based on the kit instructions.…”

Section: Methodsmentioning

confidence: 99%

The Diuretic Effects of Coconut Water by Suppressing Aquaporin and Renin–Angiotensin–Aldosterone System in Saline-Loaded Rats

et al. 2022

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The acute and prolonged diuretic effects of coconut water (CW) and the underlying mechanism were investigated with a saline-loaded rat model. In an acute diuretic experiment, CW could significantly increase urine excretion. In addition, the treatment of CW significantly increased urinary sodium and chloride ions, thereby considerably increasing the excretion of NaCl. However, the calcium concentration and pH value were not affected. In the prolonged diuretic experiment, CW dramatically increased the urine output and urine electrolyte concentrations (Na+, K+, and Cl–). Furthermore, CW could suppress the activation of renin–angiotensin–aldosterone system by decreasing serum antidiuretic hormone, angiotensin II, and aldosterone levels, and significantly increasing the serum atriopeptin level. CW treatment significantly reduced the mRNA expressions and protein levels of aquaporin 1 (AQP1), AQP2, and AQP 3. This report provided basic data for explaining the natural tropical beverage of CW as an alternative diuretic agent.

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Arecanut (Areca catechu L.) seed polyphenol improves osteoporosis via gut-serotonin mediated Wnt/β-catenin pathway in ovariectomized rats

Cited by 20 publications

References 69 publications

Tilapia nilotica Head Lipids Improved Bone Loss by Regulating Inflammation and Serum Metabolism Through Gut Microbiota in Ovariectomized Rats

Tilapia nilotica Head Lipids Improved Bone Loss by Regulating Inflammation and Serum Metabolism Through Gut Microbiota in Ovariectomized Rats

Gadus morhua Eggs Sialoglycoprotein Prevent Estrogen Deficiency-Induced High Bone Turnover by Controlling OPG/RANKL/TRAF6 Pathway and Serum Metabolism

The Diuretic Effects of Coconut Water by Suppressing Aquaporin and Renin–Angiotensin–Aldosterone System in Saline-Loaded Rats

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