Are we there yet? The long walk towards the development of efficient symbiotic associations between nitrogen-fixing bacteria and non-leguminous crops

Pankievicz, Vânia C. S.; Irving, T. B.; Maia, Lucas Gontijo Silva; Ané, Jean‐Michel

doi:10.1186/s12915-019-0710-0

Cited by 161 publications

(114 citation statements)

References 216 publications

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“…EE including a single selection cycle also proved to be very useful in understanding the bacterial genetic bases of incompatible symbiotic interactions [47,166,167] and to analyze plant-mediated selection acting on rhizobial communities [168,169]. Complementary to synthetic biology and classical genetic approaches, EE will undoubtedly become an indispensable tool to select efficient, host-adapted microsymbionts in the perspective of optimizing current symbiotic associations or transferring N 2 -fixation in crops [170,171].…”

Section: Discussionmentioning

confidence: 99%

Experimental Evolution of Legume Symbionts: What Have We Learnt?

Moura

Remigi

Masson‐Boivin

et al. 2020

Genes

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Rhizobia, the nitrogen-fixing symbionts of legumes, are polyphyletic bacteria distributed in many alpha- and beta-proteobacterial genera. They likely emerged and diversified through independent horizontal transfers of key symbiotic genes. To replay the evolution of a new rhizobium genus under laboratory conditions, the symbiotic plasmid of Cupriavidus taiwanensis was introduced in the plant pathogen Ralstonia solanacearum, and the generated proto-rhizobium was submitted to repeated inoculations to the C. taiwanensis host, Mimosa pudica L. This experiment validated a two-step evolutionary scenario of key symbiotic gene acquisition followed by genome remodeling under plant selection. Nodulation and nodule cell infection were obtained and optimized mainly via the rewiring of regulatory circuits of the recipient bacterium. Symbiotic adaptation was shown to be accelerated by the activity of a mutagenesis cassette conserved in most rhizobia. Investigating mutated genes led us to identify new components of R. solanacearum virulence and C. taiwanensis symbiosis. Nitrogen fixation was not acquired in our short experiment. However, we showed that post-infection sanctions allowed the increase in frequency of nitrogen-fixing variants among a non-fixing population in the M. pudica–C. taiwanensis system and likely allowed the spread of this trait in natura. Experimental evolution thus provided new insights into rhizobium biology and evolution.

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Section: Discussionmentioning

confidence: 99%

Experimental Evolution of Legume Symbionts: What Have We Learnt?

Moura

Remigi

Masson‐Boivin

et al. 2020

Genes

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“…Also, positive results in corn development and yield has been reported with Azospirillum brasilense inoculation (strains Ab-V5 and Ab-V6) under tropical conditions (Martins et al, 2018;Oliveira et al, 2018;Galindo et al, 2019b). However, greater responses with other PGPB can be achieved (Pankievicz et al, 2019). New research investigating Bacillus spp.…”

Section: Introductionmentioning

confidence: 99%

“…and Pseudomonas spp. as beneficial PGPB are being conducted, especially for annual crops (Oliveira et al, 2019;Pankievicz et al, 2019;Tavanti et al, 2020). For example, under tropical conditions, Bacillus subtilis inoculation (strains Pant001 and QST713) associated with Bradyrhizobium japonicum has been reported to increase soybean [Glycine max (L.) Merr.]…”

Section: Introductionmentioning

confidence: 99%

Corn Yield and Phosphorus Use Efficiency Response to Phosphorus Rates Associated With Plant Growth Promoting Bacteria

Pereira

Galindo

Gazola

et al. 2020

Front. Environ. Sci.

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The use of plant growth promoting bacteria (PGPB) that can solubilize phosphorus (P) has shown potential to improve nutrient availability in grass crops such as corn (Zea mays L.) This study was developed to investigate if inoculation with Azospirillum brasilense, Bacillus subtilis or Pseudomonas fluorescens associated with P 2 O 5 rates can improve phosphorus use efficiency (PUE) reflecting on greater corn development and yield. The field trial was set up in a Rhodic Hapludox under no-till system under Savannah conditions, in a completely randomized block design with four replicates. Treatments were tested in a full factorial design and included: (i) five P 2 O 5 rates (0 to 105 kg ha −1) and (ii) four PGPB seed inoculation (Control-without inoculation, A. brasilense, B. subtilis or P. fluorescens). Inoculation was found to increase grain yield by 39.5, 29.1, and 15.9% when B. subtilis was inoculated in the absence of P 2 O 5 rates and associated with 17.5 and 70 kg P 2 O 5 ha −1 and by 34.7% when A. brasilense was inoculated with application of 105 kg P 2 O 5 ha −1. In addition, inoculation with B. subtilis and A. brasilense were found to increase P uptake, benefiting productive components development, leading to an improved PUE, and greater corn grain yield. The results of this study showed positive improvements in P uptake as a result of B. subtilis and A. brasilense inoculation, with an increase of 100.5 and 54.6% on PUE, respectively; while the P. fluorescens inoculation were less evident. Further research should be conducted under biotic or/and abiotic conditions such as attack of pathogens and insects, drought, salinity, water flooding, low and high temperature to better understand the role of PGPB, inoculated alone or in combination as the co-inoculated method.

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“…In this sense, developing more e cient nitrogen-xing associations is of high importance given the pollution problems associated with the excessive use of chemical fertilizers (25,26). This requires a better understanding of the factors, including nutrient transport, in uencing the success of the symbiotic process (27)(28)(29). In line with this, it has been shown that every step in the establishment of Legumerhizobia symbioses is severely affected by B de ciency (30)(31)(32)(33)(34)(35).…”

Section: Introductionmentioning

confidence: 98%

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

Granado‐Rodríguez

Bolaños

Reguera

2020

Preprint

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Background: Boron (B) deficiency or toxicity in soils can cause the inhibition of growth and an altered development in plants. In such conditions, the participation of two distinct protein families (the aquaporin family Major intrinsic proteins (MIPs) and the Boron transporter family BOR) is required to minimize detrimental effects caused by B stress. Legumes comprise important crops that offer major agronomic benefits, including the capacity of establishing symbiosis with rhizobia fixing atmospheric N2 and for being important food protein sources. It has been proven that legumes are susceptible to B stress, which leads to important yield penalties. However, little is known about the transport mechanisms responsible for B uptake and distribution in legumes, especially under deficiency.Results: A Medicago truncatula protein, MtNIP5;1 (Medtr1g097840) (homologous to the Arabidopsis thaliana AtNIP5;1) was identified as a novel legume B transporter involved in B uptake under deficiency. Further analyses revealed that this M. truncatula aquaporin expression was boron-regulated in roots, being induced under deficiency and repressed under toxicity. It localizes at the plasma membrane of root epidermal cells and in nodules, where B plays pivotal roles in symbiosis. Furthermore, a partial complementation of the nip5;1 A. thaliana mutant phenotype under B deficiency supports a functional role of MtNIP5;1 as a B transporter in this legume model plant. Conclusions: The results here presented support a functional role of MtNIP5;1 in B uptake under deficiency and provides new insights into this micronutrient transport mechanisms in legume species.

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Are we there yet? The long walk towards the development of efficient symbiotic associations between nitrogen-fixing bacteria and non-leguminous crops

Cited by 161 publications

References 216 publications

Experimental Evolution of Legume Symbionts: What Have We Learnt?

Experimental Evolution of Legume Symbionts: What Have We Learnt?

Corn Yield and Phosphorus Use Efficiency Response to Phosphorus Rates Associated With Plant Growth Promoting Bacteria

MtNIP5;1, a novel Medicago truncatula boron diffusion facilitator induced under deficiency

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