Arcticibacterium luteifluviistationis gen. nov., sp. nov., isolated from Arctic seawater

Abstract

A Gram-staining-negative, aerobic, non-motile and yellow-pigmented bacterium, designated strain SM1504T, was isolated from Arctic seawater. It hydrolysed aesculin and gelatin but did not reduce nitrate to nitrite. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain SM1504T constituted a distinct phylogenetic line within the family Cytophagaceae and was closely related to species of the genera Lacihabitans, Emticicia, Fluviimonas and Leadbetterella, with respect to which low sequence similarities between 88.9 and 91.6 % were observed. The major fatty acids of strain SM1504T were summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH) and iso-C15 : 0. The predominant polar lipids of strain SM1504T were phosphatidylethanolamine and one unidentified lipid. The only respiratory quinone detected in strain SM1504T was MK7. The DNA G+C content of strain SM1504T was 40.8 mol%. On the basis of the phylogenetic, chemotaxonomic and phenotypic characterization in this study, strain SM1504T is considered to represent a novel species in a new genus of the family Cytophagaceae, for which the name Arcticibacterium luteifluviistationis gen. nov., sp. nov. is proposed. The type strain is SM1504T (=KCTC 42716T=CCTCC AB 2015348T).

Complete genome sequence of Arcticibacterium luteifluviistationis SM1504T, a cytophagaceae bacterium isolated from Arctic surface seawater

“…A total of 150 resistance genes were found to encode 24 kinds of antibiotics (such as gentamicin, kanamycin, tetracycline and streptomycin), which was consistent with the experimental antibiotic susceptibility results [ 11 ]. The genes encoding heat shock proteins dnaK and cold shock protein cspA were detected in the genome.…”

“…The genes encoding heat shock proteins dnaK and cold shock protein cspA were detected in the genome. In line with this, SM1504 T had a wider growth temperature ranges (4–30 °C) [ 11 ]. Besides, the genome harbored several genes coding for catalase and superoxide dismutase to assist the strain at cellular and molecular levels in dealing harsh radiation in the Arctic.…”

“…Then it hydrolyzed aesculin, gelatin, tyrosine, Tween 20, 40 and 60 but did not hydrolyze DNA, agar, casein, elastin, lecithin, starch, Tween 80. In addition, various enzymes such as alkaline phosphatase, esterase (C4), esterase lipase (C8), leucine arylamidase, valine arylamidase, cystine arylamidase, trypsin and glucosidase were produced for degrading organic matter [ 11 ]. The phylogenetic placement of strain SM1504 T (based on complete 16S rRNA gene sequence) through neighbor-joining phylogenetic tree was identified (Fig.…”

“…The species in the family Cytophagaceae have been isolated from various habitats including freshwater river [ 7 ], seawater [ 8 ], permafrost soil [ 9 ] and even polar glacial till [ 10 ]. The genus Arcticibacterium , belonging to the family Cytophagaceae , accommodates only one recognized species: A. luteifluviistationis SM1504 T (= KCTC 42716 T = CCTCC AB 2015348 T ) [ 11 ]. Strain SM1504 T was isolated from surface seawater of King’s Fjord, Arctic.…”

Enhanced production of biomass and lipids by Euglena gracilis via co-culturing with a microalga growth-promoting bacterium, Emticicia sp. EG3

“…Strain EG3 is the first MGPB for E. gracilis . Members of the genus Emticicia have been isolated from various environments, including freshwater [34–37], wetlands [38], seawater [39], the sediments of a shallow stream [40], and soil [41, 42]. Although the ecological role of Emticicia and its potential for industrial use are still unclear, our results reveal that one of its ecosystem services may be to function in a symbiotic relationship that enhances the growth of microalgae such as E. gracilis .…”