Simulatenous evaluation of composting experiments and metagenome analyses to illuminate the effect of Streptomyces spp. on organic matter degradation

Abstract

Streptomyces spp. were isolated via sampling during thermophilic phase of a compost system. Isolates were subjected to a careful and detailed sets of procedures in order to select best candidates effective on organic matter degradation. This effect, on organic matter degradation was illumanated by evaluation of composting experiments and metagenome analyses in accordance. Results indicated dominanace of strains when added to compost systems. This dominanace was found to be partially due to possible secondary metabolite production of isolates determined by antagonism tests. The dominance of strains was in fact distinctive in establishment of variant organic matter degradation. Enzymatic activity as a result of strains' dominance was provided as an explanation based on evaluation of metagenomic counts. Metagenome count numbers revealed the tendency of compost microbiota towards degradation products of cellulose and xylan was also determined to be least favored polysaccharide. Results also implied a decrease of competition between the dominant strain and microbita members in the case of sequential cellulose and xylan degradation. Meticulous evaluation of results obtained from metagenome analysis also provided some insights on certain conditions regarding the progress of composting along with storage conditions of manure prior to use.

Streptomyces at the Heart of Several Sectors to Support Practical and Sustainable Applications: A Review

“…The potential of Streptomyces spp in pollution degradation in effluent from paper pulp has been proven through the detection of breakdown products observed after incubation period [297] . Furthermore, a recent study done by Kocak et al [298] found that The introduction of Streptomyces spp. to the compost system resulted in significant changes, including the inhibition of other microbiota members.…”

Optimization of Thermotolerant Cellulase from Streptomyces sp. M127-1 with Response Surface Methodology using Solid-State Fermentation

“…Streptomyces'in organik madde bozulması üzerindeki etkisini aydınlatmak için kompostlama deneyleri gerçekleştirilmiş ve kompost sistemlerine eklenen suşların tüm kompost mikrobiyotasına hâkim olduğu ve bu aşamadaki en önemli faktörün enzim aktivitesi olduğu rapor edilmiştir (Kocak et al 2023). Bostubayeva ve arkadaşları (2023) yaptıkları çalışmada iki Streptomyces türünün kompostlama süreçlerinde iyi bir biyoaktivatör olacağını rapor etmişlerdir.…”

“…Yüksek selülaz aktivitesi, kompost olgunlaşmasının hızlandırılmasına yol açan selüloz bozunmasına neden olur. Selülaz enzimleri mikroorganizmaların selüloza ulaşmasını kolaylaştırarak mikrobiyal aktiviteyi ve biyokütle üretimini arttırır (Sun et al 2021, Kocak et al 2023. Ayrıca, selülozun selülaz tarafından enzimatik olarak parçalanması, karbon, azot ve fosfor gibi besinlerin salınmasına katkıda bulunarak bu bileşiklerin bitki tarafından alımını kolaylaştırır (Mazumder et al 2021).…”