Aquatic Bacillus cereus JD0404 isolated from the muddy sediments of mangrove swamps in Thailand and characterization of its cellulolytic activity

Chantarasiri, Aiya

doi:10.1016/j.ejar.2015.08.003

Cited by 36 publications

(43 citation statements)

References 30 publications

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“…The optimal cellulase temperature and thermostability which was obtained by isolate C55 was the same as obtained by B. licheniformis WBS1 and Bacillus sp. WBS2 [4], B. cereus JD0404 [5], B. amyloliquefaciens DL-3 [6] and B. subtilis subsp. Subtilis A-53 [7] and Bacillus sp.…”

Section: The Effect Of Temperature and Ph On Enzyme Activity And Stabmentioning

confidence: 99%

Characterization of thermostable cellulase produced by Bacillus strains isolated from solid waste of carrageenan

Listyaningrum

Sutrisno

Wardani

2018

IOP Conf. Ser.: Earth Environ. Sci.

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Abstract. Cellulase-producing bacteria was isolated from solid waste of carrageenan and identified as Bacillus licheniformis C55 by 16S rRNA sequencing. The optimum condition for cellulase production was obtained at pH and temperature of 8.0 and 50°C, respectively in a medium containing glucose as carbon source and 1.0% carboxymethyl cellulose (CMC) to stimulate the cellulase production. Most remarkably, the enzyme retained its relative activity over 50% after incubation at 50°C for 90 minutes. Substrate specificity suggested that the enzyme is an endoglucanase. The molecular mass of Bacillus licheniformis C55 crude cellulase was found about 18 kDa by SDS-PAGE analysis. This thermostable enzyme would facilitate development of more efficient and cost-effective forms of the process to convert lignocellulosic biomass into high-value products.

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Section: The Effect Of Temperature and Ph On Enzyme Activity And Stabmentioning

confidence: 99%

Characterization of thermostable cellulase produced by Bacillus strains isolated from solid waste of carrageenan

Listyaningrum

Sutrisno

Wardani

2018

IOP Conf. Ser.: Earth Environ. Sci.

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“…Most cellulolytic enzymes isolated from mangrove were from bacteria belonging to the genera Micrococcus, Bacillus, Pseudomonas, Xanthomonas, and Brucella [43,44]. The cellulolytic bacteria isolated from different areas have different properties.…”

Section: Isolation Screening and Identi Cation Of Cellulolytic Marimentioning

confidence: 99%

“…The selected cellulolytic isolate was identi ed by molecular genetic analysis. The PCR ampli cation and 16S rDNA sequence analysis were previously described [44] using a set of primers as follows: forward primer 27F: GAGTTTGATCATGGCTCAG and reverse primer 1492R:…”

Section: Screening and Identi Cation Of Cellulolytic Bacteriamentioning

confidence: 99%

Isolation and Characterization of Cellulolytic Marine Bacteria for Litopenaeus Vannamei Aquaculture Using Sugarcane Bagasse as Carbon Source

Wei¹,

Xu²,

Long³

et al. 2020

Preprint

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Background: In aquaculture system, it is essential to adjust the inherent disadvantage of C/N ratio by adding a lot of additional carbon sources, such as sugarcane molasses, organic acids, and organic acid salts, which will greatly increase the cost of shrimp aquaculture. Herein, we aimed to isolate cellulolytic marine bacteria to hydrolyze sugarcane bagasse (SB) for reducing the cost of addition of external carbon sources in industrial Litopenaeus vannamei aquaculture.Results: A total of 97 cellulolytic marine bacterial strains belonged to 6 genera were isolated from 2,585 indigenous bacteria, indicating that seagrass bed can be used as an important place for screening the cellulolytic bacteria. The hydrolysis capacity (HC) of 58 cellulolytic marine bacterial strains was ranged from 1.1–4.0. MW-M5 displayed the largest HC value, followed by MW-M10 and MW-M14. The cellulase contents of 30 strains were more than 3 U/g in the supernatant of fermentation broth after 24 h, which was significantly higher than that of commercial cellulose. 26 cellulolytic marine bacteria with HC greater than 2 were safe for L. vannamei. MW-M19 with the lowest multiple antibiotic resistance index, 0.1, had a highest SB enzyme activity, 4.14 U/mL. The SB decomposition rates of CFW-C18 and MW-M15 were up to about 63.81% and 48.57% after fifteen days, respectively. Conclusions: These results provide valuable information for further construction of a shrimp aquaculture system based on low-cost external carbon sources using cellulolytic bacteria, and even for other biotechnological applications.

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“…The β-glucosidase hydrolyzes cellobiose and water-soluble cellodextrin to glucose (Bhat and Bhat 1997). There have been many studies that isolate cellulose-degrading bacteria including isolation from plant litter soil (Waghmare et al 2018), sediment of mangrove swamps (Chantarasiri 2015), and the gut of termites (Upadhyaya et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell

2020

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Abstract. Khoirunnisa NS, Anwar S, Santosa DA. 2020. Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell. Biodiversitas 21: 1686-1696. Cellulose such as in rice straw can be utilized as an organic substrate in Microbial Fuel Cell (MFC) to generate electricity by microorganisms as a biocatalyst. This research aimed to get cellulose-degrading bacteria with high capability to degrade rice straw and able to be used as consortium with exoelectrogen bacteria in Microbial Fuel Cell. The stages of research included: (i) isolation of the bacteria using carboxymethylcellulose (CMC) agar medium, (ii) selection of the isolates for that purpose, (iii) enzyme assay and MFC performance test, and (iv) identification of selected isolate. There were 125 isolates that were obtained. Selection based on the ability to degrade cellulose as indicated by clear zone on CMC medium resulted in 23 isolates. Ten isolates belong to anaerobic facultative bacteria were selected. Three of them were synergistic with exoelectrogen bacteria. The three isolates were tested for exoglucanase (Avicel) and total enzyme activity (Filter Paper) with the highest results were 6.21 U/mL (isolate J404) and 5.88 U/mL (isolate J401), respectively. The optimum MFC performance was achieved by one isolate, J401, which produced highest voltage of 40.8 mV and a power density of 0.33 mW/m2. The best isolate, J401, was identified as Xanthomonas translucens based on 16S rRNA method.

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Aquatic Bacillus cereus JD0404 isolated from the muddy sediments of mangrove swamps in Thailand and characterization of its cellulolytic activity

Cited by 36 publications

References 30 publications

Characterization of thermostable cellulase produced by Bacillus strains isolated from solid waste of carrageenan

Characterization of thermostable cellulase produced by Bacillus strains isolated from solid waste of carrageenan

Isolation and Characterization of Cellulolytic Marine Bacteria for Litopenaeus Vannamei Aquaculture Using Sugarcane Bagasse as Carbon Source

Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell

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