Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell

Abstract

Abstract. Khoirunnisa NS, Anwar S, Santosa DA. 2020. Isolation and selection of cellulolytic bacteria from rice straw for consortium of microbial fuel cell. Biodiversitas 21: 1686-1696. Cellulose such as in rice straw can be utilized as an organic substrate in Microbial Fuel Cell (MFC) to generate electricity by microorganisms as a biocatalyst. This research aimed to get cellulose-degrading bacteria with high capability to degrade rice straw and able to be used as consortium with exoelectrogen bacteria in Microbial Fuel Cell. The stages of research included: (i) isolation of the bacteria using carboxymethylcellulose (CMC) agar medium, (ii) selection of the isolates for that purpose, (iii) enzyme assay and MFC performance test, and (iv) identification of selected isolate. There were 125 isolates that were obtained. Selection based on the ability to degrade cellulose as indicated by clear zone on CMC medium resulted in 23 isolates. Ten isolates belong to anaerobic facultative bacteria were selected. Three of them were synergistic with exoelectrogen bacteria. The three isolates were tested for exoglucanase (Avicel) and total enzyme activity (Filter Paper) with the highest results were 6.21 U/mL (isolate J404) and 5.88 U/mL (isolate J401), respectively. The optimum MFC performance was achieved by one isolate, J401, which produced highest voltage of 40.8 mV and a power density of 0.33 mW/m2. The best isolate, J401, was identified as Xanthomonas translucens based on 16S rRNA method.

Xác định khả năng phân giải carboxymethyl cellulose và cellulose của các vi sinh vật phân lập từ ruột mối dưới đất (termitidae) thu nhận tại huyện Mang Thít, tỉnh Vĩnh Long

“…Sự gia tăng sản xuất nông nghiệp tương quan với sự gia tăng chất thải hữu cơ, chẳng hạn như rơm rạ. Có khoảng 1149,6 triệu tấn rơm rạ trên thế giới (Khoirunnisa et al, 2020).…”

The Effect of Microwave-Assisted Alkali and Xanthomonas t ranslucens ICBB 9762 for Rice Straw Pretreatment on Electricity Generation of Microbial Fuel Cell Inoculated by Staphylococcus saprophyticus ICBB 9554

“…The rice straw was dried under sunlight and then oven at 70 °C for 4 h , the milled (particle size 100 mesh) and store in an airtight container for further use. [26] This research utilized 2 bacterial isolates, cellulolytic bacteria, Xanthomonas translucens ICBB 9762 isolated from decomposed rice straw from rice soil in Bogor, Indonesia [27], and exoelectrogen bacteria, Staphylococcus saprophyticus ICBB 9554 isolated from the rice soil ecosystem in Banten, Indonesia and have been studied for the MFC system before. The Xanthomonas translucens ICBB 9762 was maintained on CMC (Carboxymethyl Cellulose) agar (in 1 liter of media containing: 1 g KH 2 PO 4 , 0.5 g K 2 SO 4 , 0.5 g NaCl, 0.01 g FeSO 4 , 0.01 g MnSO 4 , 1 g (NH 4 )NO 3 , 10 g CMC powder, and 20 g agar) whereas Staphylococcus saprophyticus ICBB 9554 was maintained on thioglycollate agar (in 1 liter of media containing: 30 g Merck ® thioglycolate broth and 20 g agar).…”

“…Design of MFC system in this study was done as described [27] The MFC chamber was a dualchamber design that was assembled by acrylic material with a size of 6×6×7.5 cm 3 on the inside and the capacity was around 250 mL in each anode and cathode chamber. The anode and cathode chamber were separated by Nafion 117 ® Proton Exchange Membrane (PEM) (Lyntech, United States).…”

“…Some bacteria that are reported as cellulolytic bacteria such as the genera Clostridium, Cellulomonas, Cellulosimicrobium, Thermomonospora, Bacillus, Ruminococcus, Erwinia, Bacteriodes, Acetovibrio, Streptomyces, Microbispora, Fibrobacter, and Paenibacillus. [26]This research utilizes Xanthomonas translucens ICBB 9762 from previous study [27] as cellulose degrading bacteria for rice straw pretreatment.…”