Aquatic Acute Toxicity Testing Using the Nematode Caenorhabditis elegans.

Ura, Kazuhiro; Kai, Toshinori; Sakata, Sachiko; Iguchi, Taisen; Arizono, Koji

doi:10.1248/jhs.48.583

Cited by 52 publications

(27 citation statements)

References 20 publications

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“…18) In our experiments, sublethal effects of BisA on C. elegans were observed at 1 and 10 nM, levels that are over 10000-fold lower than the LC 50 value. On 100 nM BisA plates, C. elegans lost fecundity, and we could not culture them after the third generation.…”

Section: Resultsmentioning

confidence: 48%

“…17,18) Others have also proposed the use of C. elegans for evaluating aquatic or soil toxicity. However, water insoluble or slightly soluble chemicals could not be evaluated in many cases because of the experimental conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Accordingly, we have established several laboratory-scale bioassay systems to evaluate the impact of various chemicals on C. elegans. [15][16][17][18] Nonylphenol (NP) and bisphenol A (BisA) are known to bind estrogen receptors. Yamakoshi et al reported previously that 4-(1-adamantyl) phenol (AdP) and 4,4′-(1,3-adamantanediyl) diphenol (AdDP) exhibited potent estrogenic activity.…”

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confidence: 99%

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A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans

Tominaga

Kohra

Iguchi

et al. 2003

JOURNAL OF HEALTH SCIENCE

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The nematode Caenorhabditis elegans (C. elegans) was used for a multiple-generation toxicity bioassay of phenols. We examined the sublethal toxicity (fecundity and reproduction) of bisphenol A (BisA), 4-(1-adamantyl) phenol (Adp), and 4,4′-(1,3-adamantanediyl) diphenol (AdDP) over five generations using a Nematode Growth Medium (NGM) 1.7% agar plate. In the fourth generation, the phenols affected the fecundity rate of C. elegans at doses 100-to 10000-fold lower than the LC 50 s. In particular, at 1 nM BisA, the number of worms decreased significantly to about 50% of control (p < 0.05). A comparison of the number of viable worms and eggs suggested that the phenols exert hatching toxicity. In addition, individuals with an abnormality in the vulva, which could not ovulate, appeared on the phenol-containing plates. We confirmed that phenols disrupt reproduction in C. elegans and that the method using NGM agar plates facilitates multi-generational toxicity tests of chemicals that are poorly soluble in water.

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Section: Resultsmentioning

confidence: 48%

Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans

Tominaga

Kohra

Iguchi

et al. 2003

JOURNAL OF HEALTH SCIENCE

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“…The control consisted of 1% bacteriological agar with a final concentration of 1% DMSO. We chose to keep the concentration of DMSO in the final solutions at 1% to exclude any influence on the mortality rate as reported for Caenorhabditis elegans by Ura et al (2002) for DMSO concentrations in excess of 5%. Final drug concentrations in the wells were 1, 10, 20, 50 and 100 μg ml − 1 for TBZ and 0·01, 0·1, 0·5, 1·5, and 2 μg ml − 1 for IVM.…”

Section: Methodsmentioning

confidence: 99%

Anthelmintic tolerance in free-living and facultative parasitic isolates ofHalicephalobus(Panagrolaimidae)

et al. 2012

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S U M M A R YStudies on anthelmintic resistance in equine parasites do not include facultative parasites. Halicephalobus gingivalis is a freeliving bacterivorous nematode and a known facultative parasite of horses with a strong indication of some form of tolerance to common anthelmintic drugs. This research presents the results of an in vitro study on the anthelmintic tolerance of several isolates of Halicephalobus to thiabendazole and ivermectin using an adaptation of the Micro-Agar Larval Development Test hereby focusing on egg hatching and larval development. Panagrellus redivivus and Panagrolaimus superbus were included as a positive control. The results generally show that the anthelmintic tolerance of Halicephalobus to both thiabendazole and ivermectin was considerably higher than that of the closely related Panagrolaimidae and, compared to other studies, than that of obligatory equine parasites. Our results further reveal a remarkable trend of increasing tolerance from fully freeliving isolates towards horse-associated isolates. In vitro anthelmintic testing with free-living and facultative parasitic nematodes offers the advantage of observing drug effect on the complete life cycle as opposed to obligatory parasites that can only be followed until the third larval stage. We therefore propose Halicephalobus gingivalis as an experimental tool to deepen our understanding of the biology of anthelmintic tolerance.

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“…In addition, no survival was observed in the 10.0% DMSO exposures after 24h. Based on the results of this exposure and those of previously published studies (Okumura et al, 2001, Ura et al, 2002, the use of DMSO in final concentrations ≤ 1.0% appear to be appropriate for performing 96h-acute toxicity testing. In this study with B(a)P we applied a much lower solvent concentration, i.e.…”

Section: Solvent Toxicity Of Dmsomentioning

confidence: 67%

Testosterone metabolism in Neomysis integer following exposure to benzo(a)pyrene

Poelmans¹,

Verslycke²,

Monteyne³

et al. 2006

Comparative Biochemistry and Physiology Part B: Biochemistry an

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Cytochromes P450 (CYPs) are important enzymes involved in the regulation of hormone synthesis and in the detoxification and/or activation of xenobiotics. CYPs are found in virtually all organisms, from archae, and eubacteria to eukaryota. A number of endocrine disruptors are suspected of exerting their effects through disruption of normal CYP function.Consequently, alterations in steroid hormone metabolism through changes in CYP could provide an important tool to evaluate potential effects of endocrine disruptors. The aim of this study was to investigate the potential effects of the known CYP modulator, benzo(a)pyrene (B(a)P), on the testosterone metabolism in the invertebrate Neomysis integer (Crustacea; Mysidacea). N. integer were exposed for 96h to 0.43, 2.39, 28.83, 339.00 and 1682.86µg B(a)P L -1 and a solventcontrol, and subsequently their ability to metabolize testosterone was assessed. Identification and quantification of the produced phase I and phase II testosterone metabolites was performed using liquid chromatography coupled with multiple mass spectrometry (LC-MS 2 ). Significant changes were observed in the overall ability of N. integer to metabolize testosterone when exposed to 2.39, 28.83, 339.00 and 1682.86µg B(a)P L -1 as compared to the control animals.

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Aquatic Acute Toxicity Testing Using the Nematode Caenorhabditis elegans.

Cited by 52 publications

References 20 publications

A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans

A Multi-Generation Sublethal Assay of Phenols Using the Nematode Caenorhabditis elegans

Anthelmintic tolerance in free-living and facultative parasitic isolates ofHalicephalobus(Panagrolaimidae)

Testosterone metabolism in Neomysis integer following exposure to benzo(a)pyrene

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