Testosterone metabolism in Neomysis integer following exposure to benzo(a)pyrene

Abstract

Cytochromes P450 (CYPs) are important enzymes involved in the regulation of hormone synthesis and in the detoxification and/or activation of xenobiotics. CYPs are found in virtually all organisms, from archae, and eubacteria to eukaryota. A number of endocrine disruptors are suspected of exerting their effects through disruption of normal CYP function.Consequently, alterations in steroid hormone metabolism through changes in CYP could provide an important tool to evaluate potential effects of endocrine disruptors. The aim of this study was to investigate the potential effects of the known CYP modulator, benzo(a)pyrene (B(a)P), on the testosterone metabolism in the invertebrate Neomysis integer (Crustacea; Mysidacea). N. integer were exposed for 96h to 0.43, 2.39, 28.83, 339.00 and 1682.86µg B(a)P L -1 and a solventcontrol, and subsequently their ability to metabolize testosterone was assessed. Identification and quantification of the produced phase I and phase II testosterone metabolites was performed using liquid chromatography coupled with multiple mass spectrometry (LC-MS 2 ). Significant changes were observed in the overall ability of N. integer to metabolize testosterone when exposed to 2.39, 28.83, 339.00 and 1682.86µg B(a)P L -1 as compared to the control animals.

Endogenous boldenone-formation in cattle: Alternative invertebrate organisms to elucidate the enzymatic pathway and the potential role of edible fungi on cattle's feed

“…Especially with regard to bBol, reproducibility of in vivo exposure experiments with N. integer has been reported by De Wasch et al [33] to be poor. In contrast, the use of N. integer's testosterone-metabolism has proven its use in eco-toxicological studies [44][45][46]57]. For the purpose of simulating vertebrate metabolic pathways however, another invertebrate L. sericata, was evaluated as model organism [32].…”

“…During exposure, test organisms were placed in 5-mL glass tubes in a temperature-controlled chamber (15 • C, Liebher ® , Laborimpex, Brussels) [44][45][46].…”

THE BIOTRANSFORMATION, BIODEGRADATION, AND BIOREMEDIATION OF ORGANIC COMPOUNDS BY MICROALGAE1

“…2003). These studies provide novel insights into our understanding of steroid metabolic pathways, which evaluated the species’ differences in their ability to transform steroid compounds (Poelmans et al. 2006).…”

Short-term toxicity of polystryrene microplastics on mysid shrimps Neomysis japonica

“…Entanglement and blocking of PS-MPs in the tissues may disrupt physiological processes [6], hamper energy flows [7] and even affect the survival and growth of marine biota [8]. Mysid shrimps (Neomysis japonica), as an ecotoxicological model for environmental sensitivity [9], have been used in evaluating toxicity of heavy metal [10], PAHs [11] …”