Aptamer Cell-Based Selection: Overview and Advances

Catuogno, Silvia; Esposito, Carla Lucia

doi:10.3390/biomedicines5030049

Cited by 66 publications

(51 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The process generally includes counter-selection steps against unwanted targets in order to improve the aptamer selectivity. To date, many variants of SELEX have been described and applied to a wide range of different targets from purified proteins and small molecules to complex intact cells, tissues or live animals [ 13 ]. At the end of the selection, individual sequences are isolated by cloning or sequencing and are screened for the binding affinity and selectivity for the chosen target.…”

Section: Selex Technologies To Generate Nsclc Targeting Aptamersmentioning

confidence: 99%

See 1 more Smart Citation

Advances in Oligonucleotide Aptamers for NSCLC Targeting

Rotoli

Santana-Viera

Ibba

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Non-small-cell lung cancer (NSCLC) is the most common type of lung cancer worldwide, with the highest incidence in developed countries. NSCLC patients often face resistance to currently available therapies, accounting for frequent relapses and poor prognosis. Indeed, despite great recent advancements in the field of NSCLC diagnosis and multimodal therapy, most patients are diagnosed at advanced metastatic stage, with a very low overall survival. Thus, the identification of new effective diagnostic and therapeutic options for NSCLC patients is a crucial challenge in oncology. A promising class of targeting molecules is represented by nucleic-acid aptamers, short single-stranded oligonucleotides that upon folding in particular three dimensional (3D) structures, serve as high affinity ligands towards disease-associated proteins. They are produced in vitro by SELEX (systematic evolution of ligands by exponential enrichment), a combinatorial chemistry procedure, representing an important tool for novel targetable biomarker discovery of both diagnostic and therapeutic interest. Aptamer-based approaches are promising options for NSCLC early diagnosis and targeted therapy and may overcome the key obstacles of currently used therapeutic modalities, such as the high toxicity and patients’ resistance. In this review, we highlight the most important applications of SELEX technology and aptamers for NSCLC handling.

show abstract

Section: Selex Technologies To Generate Nsclc Targeting Aptamersmentioning

confidence: 99%

“…Typically, aptamers are produced by a process of combinatorial chemistry known as systematic evolution of ligands by exponential enrichment (SELEX) [ 11 , 12 ] that can be applied to a wide range of targets, from purified proteins to whole living cells or tissues and organisms [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Advances in Oligonucleotide Aptamers for NSCLC Targeting

Rotoli

Santana-Viera

Ibba

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Aptamers are developed in vitro via SELEX (Systematic evolution of Ligands by Exponential Enrichment), a method based on combinatorial chemistry which allows selection of high affinity DNA/RNA aptamers to the target molecule . Aptamers, for their unique ability to form secondary and tertiary structures, can recognize and bind a wide variety of target molecules, such as small molecules, proteins, cells and whole tissue . They also possess several advantages over antibodies, including higher stability, in vitro synthesis, easy chemical modification and lower immunogenicity.…”

Section: Figurementioning

confidence: 99%

“…[9,10] Aptamers, for their unique ability to form secondary and tertiary structures, can recognize and bind a wide variety of target molecules, such as small molecules, proteins, cells and whole tissue. [11] They also possess several advantages over antibodies, including higher stability, in vitro synthesis, easy chemical modification and lower immunogenicity. Therefore, aptamers are promising molecules for the development of specific, robust and cheaper sensing strategies with application in biomedicine.…”

mentioning

confidence: 99%

Rapid Detection of AIB1 in Breast Cancer Cells Based on Aptamer‐Functionalized Nanomotors

et al. 2019

View full text Add to dashboard Cite

Herein, we report ultrasound‐propelled graphene‐oxide coated gold nanowire motors, functionalized with fluorescein‐labeled DNA aptamers (FAM‐AIB1‐apt), for qualitative detection of overexpressed AIB1 oncoproteins in MCF‐7 breast cancer cells. The movement of nanomotors under the ultrasound field facilitated intracellular uptake and resulted in a faster aptamer binding with the target protein and thus faster fluorescence recovery. The propulsion behavior of the aptamer functionalized nanomotors greatly enhanced the fluorescence intensity compared to static conditions. The new aptamer@nanomotor‐based strategy offers considerable potential for further development of sensing methodologies towards diagnosis of breast cancer.

show abstract

“…This result was not unexpected. While aptamers are known for their remarkable stabilities to bind their target over wide range of pH and temperatures (Catuogno & Esposito, 2017), it is also reported that significant pH changes can in fact reduce their detection sensitivities due to possible changes in their secondary structures, thus affecting target binding efficiency (Hianik, Ostatná, Sonlajtnerova, & Grman, 2007). This was likely the case for detection in orange juice (pH 4.0).…”

Section: Detection With Rca Signal Intensificationmentioning

confidence: 99%

Rolling circle amplification and its application in microfluidic systems for Escherichia coli O157:H7 detections

Jiang

Qiu

et al. 2019

Journal of Food Safety

View full text Add to dashboard Cite

Rolling circle amplification (RCA) has been widely used to enhance detection signals as its long single‐stranded RCA products can provide multiple binding sites for signal probes for sensitive detections. In the current study, we employ atomic force microscopy (AFM) to monitor the RCA products during the course of the RCA process over time. Subsequently, the results of the RCA obtained from the AFM study are combined with those from the conventional electrophoresis method to optimize RCA reactions for rapid and sensitive detection. We show that there appears to be an inhomogeneous RCA initiation phase in early to mid‐stage of the RCA reaction where some chains grow faster while others grow slower or remain dormant, an observation that has not been reported in the literature. Furthermore, we demonstrate that the RCA can significantly enhance detection signals by up to 100‐fold. We also show that the Escherichia coli O157:H7 detection with the RCA can be carried out in different food matrices with excellent detection sensitivities and specificities. In conclusion, these results suggest that a microfluidic device in combination with RCA signal enhancement is a simple and robust approach to sensitive whole‐cell detection in food samples. Practical applications The current research has exciting potentials for applications in sensitive detections of food samples for food safety inspections.

show abstract

Aptamer Cell-Based Selection: Overview and Advances

Cited by 66 publications

References 103 publications

Advances in Oligonucleotide Aptamers for NSCLC Targeting

Advances in Oligonucleotide Aptamers for NSCLC Targeting

Rapid Detection of AIB1 in Breast Cancer Cells Based on Aptamer‐Functionalized Nanomotors

Rolling circle amplification and its application in microfluidic systems for Escherichia coli O157:H7 detections

Contact Info

Product

Resources

About