Rapid Detection of AIB1 in Breast Cancer Cells Based on Aptamer‐Functionalized Nanomotors

Beltrán‐Gastélum, Mara; Ávila, Berta Esteban‐Fernández de; Gong, Hua; Venugopalan, P.; Hianik, Tibor; Wang, Joseph; Šubjaková, Veronika

doi:10.1002/cphc.201900844

Cited by 41 publications

(46 citation statements)

References 29 publications

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“…A similar approach of intracellular sensing was reported for the detection of E6 miRNA in the human papilloma virus associated with oropharyngeal cancer [230]. Subsequently, Beltrán-Gastélum et al [17] also reported a similar sensing strategy for qualitative detection of cancer biomarker, known as amplified in breast cancer (AIB1), and frequently overexpressed in breast cancer (Figure 15). GO modified AuNWs were functionalized with fluorescein labeled aptamer specific to AIB1 (FAM-AIB1-apt) and propelled towards MCF7 cells.…”

Section: Nanomotors Modified By Aptamersmentioning

confidence: 74%

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Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

2021

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Polymer nanoparticles and nano/micromotors are novel nanostructures that are of increased interest especially in the diagnosis and therapy of cancer. These structures are modified by antibodies or nucleic acid aptamers and can recognize the cancer markers at the membrane of the cancer cells or in the intracellular side. They can serve as a cargo for targeted transport of drugs or nucleic acids in chemo- immuno- or gene therapy. The various mechanisms, such as enzyme, ultrasound, magnetic, electrical, or light, served as a driving force for nano/micromotors, allowing their transport into the cells. This review is focused on the recent achievements in the development of polymer nanoparticles and nano/micromotors modified by antibodies and nucleic acid aptamers. The methods of preparation of polymer nanoparticles, their structure and properties are provided together with those for synthesis and the application of nano/micromotors. The various mechanisms of the driving of nano/micromotors such as chemical, light, ultrasound, electric and magnetic fields are explained. The targeting drug delivery is based on the modification of nanostructures by receptors such as nucleic acid aptamers and antibodies. Special focus is therefore on the method of selection aptamers for recognition cancer markers as well as on the comparison of the properties of nucleic acid aptamers and antibodies. The methods of immobilization of aptamers at the nanoparticles and nano/micromotors are provided. Examples of applications of polymer nanoparticles and nano/micromotors in targeted delivery and in controlled drug release are presented. The future perspectives of biomimetic nanostructures in personalized nanomedicine are also discussed.

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Section: Nanomotors Modified By Aptamersmentioning

confidence: 74%

“…Interaction of aptamer with cancer markers caused its folding, moving the fluorescent probe away from the GO. This is accompanied by increase of fluorescence [17].…”

Section: Introductionmentioning

confidence: 89%

Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

2021

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“…Besides the conventional methods such as IHC and fluorescent in situ hybridization (FISH), the new techniques will be developed to measure the expression and amplification of the SRC-3 gene. A recent study invented a new method for monitoring SRC-3 levels in a breast cancer cell based on aptamer-functionalized nanomotors [ 174 ]. However, whether this method can be used to detect SRC-3 in tissues needs to be further clarified.…”

Section: Discussionmentioning

confidence: 99%

SRC-3, a Steroid Receptor Coactivator: Implication in Cancer

Li¹,

Deng²,

Chen³

2021

IJMS

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Steroid receptor coactivator-3 (SRC-3), also known as amplified in breast cancer 1 (AIB1), is a member of the SRC family. SRC-3 regulates not only the transcriptional activity of nuclear receptors but also many other transcription factors. Besides the essential role of SRC-3 in physiological functions, it also acts as an oncogene to promote multiple aspects of cancer. This review updates the important progress of SRC-3 in carcinogenesis and summarizes its mode of action, which provides clues for cancer therapy.

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“…For example, fluorescein-labeled aptamers conjugated with an ultrasound-propelled gold nanowire motors (FAM-AIB1-apt) coated with graphene-oxide (C 140 H 42 O 20 ) was used for qualitative diagnosis of overexpressed AIB1 oncoproteins in breast cancer cells (MCF-7). The nanomotor movement in the ultrasound field ensured the faster uptake and binding of the aptamers with the target and results in fluorescence [ 50 ]. The propulsion of the nanomotors conjugated with aptamer significantly increased the fluorescence intensity, in comparison to the static conditions.…”

Section: Aptamers In Cancer Diagnosismentioning

confidence: 99%

Current Perspectives on Aptamers as Diagnostic Tools and Therapeutic Agents

2020

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Aptamers are synthetic single-stranded DNA or RNA sequences selected from combinatorial oligonucleotide libraries through the well-known in vitro selection and iteration process, SELEX. The last three decades have witnessed a sudden boom in aptamer research, owing to their unique characteristics, like high specificity and binding affinity, low immunogenicity and toxicity, and ease in synthesis with negligible batch-to-batch variation. Aptamers can specifically bind to the targets ranging from small molecules to complex structures, making them suitable for a myriad of diagnostic and therapeutic applications. In analytical scenarios, aptamers are used as molecular probes instead of antibodies. They have the potential in the detection of biomarkers, microorganisms, viral agents, environmental pollutants, or pathogens. For therapeutic purposes, aptamers can be further engineered with chemical stabilization and modification techniques, thus expanding their serum half-life and shelf life. A vast number of antagonistic aptamers or aptamer-based conjugates have been discovered so far through the in vitro selection procedure. However, the aptamers face several challenges for its successful clinical translation, and only particular aptamers have reached the marketplace so far. Aptamer research is still in a growing stage, and a deeper understanding of nucleic acid chemistry, target interaction, tissue distribution, and pharmacokinetics is required. In this review, we discussed aptamers in the current diagnostics and theranostics applications, while addressing the challenges associated with them. The report also sheds light on the implementation of aptamer conjugates for diagnostic purposes and, finally, the therapeutic aptamers under clinical investigation, challenges therein, and their future directions.

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Rapid Detection of AIB1 in Breast Cancer Cells Based on Aptamer‐Functionalized Nanomotors

Cited by 41 publications

References 29 publications

Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

Polymer Nanoparticles and Nanomotors Modified by DNA/RNA Aptamers and Antibodies in Targeted Therapy of Cancer

SRC-3, a Steroid Receptor Coactivator: Implication in Cancer

Current Perspectives on Aptamers as Diagnostic Tools and Therapeutic Agents

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