Aptamer-based electrochemical biosensor for highly sensitive and selective malaria detection with adjustable dynamic response range and reusability

Figueroa-Miranda, Gabriela; Feng, Lingyan; Shiu, Simon Chi‐Chin; Dirkzwager, Roderick M.; Cheung, Yiu-ming; Tanner, Julian A.; Schöning, Michael J.; Offenhäusser, Andreas; Mayer, Dirk

doi:10.1016/j.snb.2017.07.117

Cited by 87 publications

(42 citation statements)

References 32 publications

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“…There seems to be a growing trend of aptamer-based sensors targeting pLDH [54,55,89]. Compared to antibodies, aptamers are smaller in size, thermostable, extended shelf life without functional degradation, affordable, easily synthesized and can be readily modified.…”

Section: Detection Of Pldh In Clinical Samplesmentioning

confidence: 99%

“…Native pLDH in clinical samples was also detected up to 1 parasite/µL. Figueroa-Miranda et al immobilized 2008s aptamers on a SAM-modified gold electrode to bind pLDH down to the detection limit of 0.84 pM in buffer and 1.30 pM in blood ( Figure 3, Table 2) [89]. The aptasensor had a detection range between 1 pM-10 nM and remained highly selective for PfLDH even in the presence of high concentrations of serum proteins and analogues of LDH from muse muscle.…”

Section: Detection Of Pldh In Clinical Samplesmentioning

confidence: 99%

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Recent Advances in the Development of Biosensors for Malaria Diagnosis

Krampa

Aniweh

Kanyong

et al. 2020

Sensors

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The impact of malaria on global health has continually prompted the need to develop more effective diagnostic strategies that could overcome deficiencies in accurate and early detection. In this review, we examine the various biosensor-based methods for malaria diagnostic biomarkers, namely; Plasmodium falciparum histidine-rich protein 2 (PfHRP-2), parasite lactate dehydrogenase (pLDH), aldolase, glutamate dehydrogenase (GDH), and the biocrystal hemozoin. The models that demonstrate a potential for field application have been discussed, looking at the fabrication and analytical performance characteristics, including (but not exclusively limited to): response time, sensitivity, detection limit, linear range, and storage stability, which are first summarized in a tabular form and then described in detail. The conclusion summarizes the state-of-the-art technologies applied in the field, the current challenges and the emerging prospects for malaria biosensors.Sensors 2020, 20, 799 2 of 21 emergence and spread of drug resistance. It could also reduce the pool of individuals who can contribute to malaria transmission [5].To date, many technologies have attempted to circumvent the challenges in malaria diagnostics with technologies that address point-of-care needs and early stage asymptomatic detection. In this review, we first comprehensively summarize the biomarkers targeted during the course of malaria with emphasis on the importance of sensitive early detection. Next, we provide an overview of the recent advances in biosensor technologies for the detection of the most targeted biomarkers, focusing on: development, analytical performances, and suitability for point of care testing. The prevailing challenges and future outlook of the use of these technologies in the field are also highlighted. Parasite Development in Humans, Biomarkers, and DiagnosisThe developmental cycle of Plasmodium species that infect humans is briefly illustrated in Figure 1 [6]. The cycle begins with the injection of sporozoites into the host's circulation by an infected female Anopheles mosquito. The sporozoites then target and enter hepatocytes where they multiply and differentiate into merozoites. This stage of the parasite life cycle is known as pre-erythrocytic. In infections involving P. vivax and P. ovale, dormant forms of the liver stage, called hypnozoites may persist in the liver [7] and cause relapse of the infection, thereby making it difficult to eradicate. The pre-erythrocytic stage is essential in the establishment of malaria infection. This stage is asymptomatic, however, it is difficult for diagnostic tools to detect sporozoites because hepatocytes invasion occurs within 30-45 min after sporozoites are inoculated by the infected mosquito [8,9]. This short time and low numbers of sporozoites injected leaves little time for their detection. Some efforts in finding biomarkers for detection of early liver stage or the dormant form have identified the Plasmodium liver-specific protein 2 (LISP2) [10,11]. The sporozoite surface circum...

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Section: Detection Of Pldh In Clinical Samplesmentioning

confidence: 99%

Section: Detection Of Pldh In Clinical Samplesmentioning

confidence: 99%

Recent Advances in the Development of Biosensors for Malaria Diagnosis

Krampa

Aniweh

Kanyong

et al. 2020

Sensors

View full text Add to dashboard Cite

show abstract

“…Additionally, the development of aptamers using species specific epitopes allowed to isolate an aptamer that could accurately discriminate between the above species [47]. Aptamers against PfLDH were used in impedance spectroscopy setups allowing to propose electrochemical sensors that recognize the biomarker at a wide range of conditions [54]. Measurements of intramolecular FRET between aptamer linked dyes allowed the development of aptamer beacons [55].…”

Section: Discussionmentioning

confidence: 99%

DNA aptamers for the recognition of HMGB1 from Plasmodium falciparum

Joseph

Nakamoto

Ruiz

et al. 2019

Preprint

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21 Rapid Diagnostic Tests (RDTs) for malaria are restricted to a few biomarkers and antibody-22 mediated detection. However, the expression of commonly used biomarkers varies 23 geographically and the sensibility of immunodetection can be affected by batch-to-batch 24 differences or limited thermal stability. In this study we aimed to overcome these limitations 25 by identifying a potential biomarker and by developing molecular sensors based on aptamer 26 technology. Using gene expression databases, ribosome profiling analysis, and structural 27 modeling, we find that the High Mobility Group Box 1 protein (HMGB1) of Plasmodium 28 falciparum is highly expressed, structurally stable and steadily present along all blood-stages 29 of P. falciparum infection. To develop biosensors, we used in vitro evolution techniques to 30 produce DNA aptamers for the recombinantly expressed HMG-box, the conserved domain 31 of HMGB1. An evolutionary approach for evaluating the dynamics of aptamer populations 32 suggested three predominant aptamer motifs. Representatives of the aptamer families were 33 tested for binding parameters to the HMG-box domain using microscale thermophoresis and 34 rapid kinetics. Dissociation constants of the aptamers varied over two orders of magnitude 35 between nano-and micromolar ranges while the aptamer-HMG-box interaction occurred in 36 less than 30 seconds. The specificity of aptamer binding to the HMG-box of P. falciparum 37 compared to its human homolog depended on pH conditions. Altogether, our study proposes 38 HMGB1 as a potential biomarker and a set of sensing aptamers that can be further developed 39 into rapid diagnostic tests for P. falciparum detection. 40 41 42 3 43 44 45 46 47 Malaria is an infectious disease that affects animals and humans, caused by protozoans of the 48 genus Plasmodium. Malaria remains the cause of 435,000 deaths worldwide, with 219 49 million cases reported during 2017 [1]. Accurate treatment requires the identification of the 50 parasite of the genus Plasmodium, in addition to the species causing the disease. Currently, 51 there are several methods to diagnose malaria, such as PCR-based, Giemsa microscopy, and 52 Rapid Diagnostic Tests (RDTs). Among these options, the last two appear to be suitable for 53 low-income and mostly affected countries; nonetheless, they also present certain limitations 54 [2]. 55 Giemsa microscopy is inexpensive to perform, can differentiate malaria species and stages, 56 and can quantify the parasites [2]. However, well-trained personnel is required and, 57 frequently unavailable in low-income countries [2]. RDTs, on the other hand, 58 detect Plasmodium spp. biomarker antigens in a small amount of blood using immobilized 59 antibodies. The two most used biomarkers are histidine-rich protein 2 (HRP-2) and lactate 60 dehydrogenase (LDH), which have been shown of high sensitivity and specificity for 61 detecting P. falciparum [3]. However, P. falciparum strains lacking the HRP-2 and HRP-3 62 genes have appeared, increasing false negative re...

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“…Aptamers can act as recognition elements, filling many of the same roles as antibodies; however, aptamers have several advantages over antibodies that make them attractive for therapeutic and biotechnological applications [ 4 ]. These include ease of chemical modification, improved stability over time and at variable pH or temperature, reversible conformation change in response to stimulus, and quick and reliable synthesis in vitro [ 5 , 6 ]. Because of these properties, much work has been done on expanding the applications of aptamers and combining them with other functional elements [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

pH-Control in Aptamer-Based Diagnostics, Therapeutics, and Analytical Applications

Belleperche

DeRosa

2018

Pharmaceuticals

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Aptamer binding has been used effectively for diagnostics, in-vivo targeting of therapeutics, and the construction and control of nanomachines. Nanostructures that respond to pH by releasing or changing affinity to a target have also been used for in vivo delivery, and in the construction of sensors and re-usable nanomachines. There are many applications that use aptamers together with pH-responsive materials, notably the targeted delivery of chemotherapeutics. However, the number of reported applications that directly use pH to control aptamer binding is small. In this review, we first discuss the use of aptamers with pH-responsive nanostructures for chemotherapeutic and other applications. We then discuss applications that use pH to denature or otherwise disrupt the binding of aptamers. Finally, we discuss motifs using non-canonical nucleic acid base pairing that can shift conformation in response to pH, followed by an overview of engineered pH-controlled aptamers designed using those motifs.

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Aptamer-based electrochemical biosensor for highly sensitive and selective malaria detection with adjustable dynamic response range and reusability

Cited by 87 publications

References 32 publications

Recent Advances in the Development of Biosensors for Malaria Diagnosis

Recent Advances in the Development of Biosensors for Malaria Diagnosis

DNA aptamers for the recognition of HMGB1 from Plasmodium falciparum

pH-Control in Aptamer-Based Diagnostics, Therapeutics, and Analytical Applications

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