Applying the Taguchi Method to the Optimization of Anticancer Activity of Bacterial Alginate-CuO Bionanocomposite

Abstract

AIM:In recent decades, despite various types of cancer inflicting many people worldwide, the existing therapies are not satisfactory and have many side effects. The present study was conducted to optimise the synthesis of novel alginate-CuO nanocomposite with utmost anticancer activity.METHODS:In this study, 9 nanocomposites were designed using Taguchi method and three factors including copper oxide nanoparticles, alginate biopolymer and stirring times were assessed at three different levels. The anticancer activity of the synthesised nanocomposites was evaluated on the MCF-7 cell line using the MTT method. Using the Qulitek-4 software, we determined the optimum conditions for the synthesis of alginate-CuO nanocomposite with the highest anticancer activity.RESULTS:The results indicated that all three factors (copper oxide, alginate and stirring time) were effective on the anticancer activity of the alginate-CuO nanocomposite. Also, the nanocomposite produced under the conditions of experiment 9 (8 mg/ml of copper oxide, 2 mg/ml of alginate and 60 min of stirring time) provided the highest growth inhibition rate as 75.63% against cancer cells.CONCLUSION:The synthesised alginate-copper oxide nanocomposites in this study showed a significant anticancer effect. Therefore, the synthesised nanocomposite under optimal conditions can be used in the design of new anticancer drugs.

Succinct and Expeditious Synthesis of Cu2O Nanoparticles by Using Liquid Jaggery: Anticancer and Wound Healing Activity Analyses

“…Cancer is a worrisome health concern that has swiftly overtaken every additional factor for mortality worldwide. The rising number of cancer cases might be attributed to poor nutrition, unhealthy diet, consumption of tobacco and alcohol [54,55] . Cancer is considered one of the most prevalent causes of fatalities in the USA, with anticipated 1.9 million instances and 609,360 fatalities due to the disease in 2022 [56] .…”

“…The rising number of cancer cases might be attributed to poor nutrition, unhealthy diet, consumption of tobacco and alcohol. [54,55] Cancer is considered one of the most prevalent causes of fatalities in the USA, with anticipated 1.9 million instances and 609,360 fatalities due to the disease in 2022. [56] Having the second-highest worldwide prevalence percentage (11.6 %) of all malignancies, breast cancer (BC) remains the leading prevalent malignancy in female worldwide and constitutes the primary reason for cancer-associated fatalities in women, necessitating the development of efficient treatments.…”

Study the effect of purified alginate from Pseudomonas aeruginosa on Hep G2 and A549 cell lines.

“…The effects of factors including copper oxide nanoparticles, alginate biopolymer and stirring time on the inhibitory effect on the growth of MCF-7 cancer cells in the structure of alginate-copper oxide nanocomposite were 69.01%, 66.04% and 65.80%, respectively. Hence the synthesized nanocomposite can be used in the design of new anticancer drugs under optimal conditions (18) Figure ( 4): Cytotoxicity effect of purifi ed alginate on A549 and WRL68 cells after 24 and 72 hrs of incubation at37ºC…”