Study the effect of purified alginate from Pseudomonas aeruginosa on Hep G2 and A549 cell lines.

Abstract

Alginate was extracted from highest alginate producer of P. aeruginosa 29 isolate, it was determined by biochemical method (Carbazole-borate assay) and molecular method (PCR), where the amplified algD gene samples showed that clear bands at one level for all the produced isolates and molecular weight were 313 on the leader scale. partially purified of alginate using gel filtration chromatography (Sephadex G-200) showed the present of single peak and initial alginate fraction at 17 to last fraction 28 of alginate concentration , where the highest alginate concentration was at fraction 23 . The cytotoxic effect of different concentrations of purified alginate on cancer cells (HePG2 , A549 ) and normal cells WRL68 was tested. The result of half-maximal inhibition concentration (IC 50) values of purified alginate treated WRL68 and HepG2 cells after 24,48,72 hours of incubation at 37º C were ( 187.8 , 62.0, 83.07) μg /ml , (102.3, 42.5, 61.03) μg/ml as respectively. While, the IC50 of purified alginate treated WRL68 and A549 cells after 24,48,72 hours of incubation at 37ºC were ( 126.2, 63.57, 90.31 ) μg /ml , (117.8, 57.06, 65.7) μg /ml as respectively.