Applications of DNA Typing

Butler, John M.

doi:10.1016/b978-0-12-374999-4.00017-5

Cited by 62 publications

(112 citation statements)

References 89 publications

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“…There were no height restrictions for the complex pull-up and -A artefacts. For a definition and explanation of the previous terms we refer to [1].…”

Section: Data Generation and Representationmentioning

confidence: 99%

“…allele. A DNA STR profile will likely contain the following elements: the desired DNA signal, disturbance due to the instrument, non-specific amplification, or other reasons, often termed noise, and artefacts, such as stutter, -A, and pull-up [1]. The interpretation of DNA profiles, especially from low DNA input mass samples, is complicated, because of the distortion of the DNA signal by the latter elements.…”

Section: Introductionmentioning

confidence: 99%

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Probabilistic characterisation of baseline noise in STR profiles

Monich

Duffy

Médard

et al. 2015

Forensic Science International: Genetics

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“…There were no height restrictions for the complex pull-up and -A artefacts. For a definition and explanation of the previous terms we refer to [1].…”

Section: Data Generation and Representationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Probabilistic characterisation of baseline noise in STR profiles

Monich

Duffy

Médard

et al. 2015

Forensic Science International: Genetics

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“…X-STR haplotyping can be of particular help in kinship testing with deficient paternity cases where a DNA sample from one of the parents is not available for testing. For example, if a father/daughter parentage relationship is in question, X-STRs may be helpful [53][54][55][56][57].…”

Section: X-chromosomal Strs and Ministrs: Identity Testing And Beyondmentioning

confidence: 99%

“…However, the problem in their approach is that for any meaningful results, a large quantity of sample is required to perform analysis with so many miniplexes, thus defeating the very purpose of miniSTRs. Samples from a mass disaster site or from an environmentally exposed crime scene may not be only highly degraded but also in very scarce quantity making it difficult for the scientist to perform multiple PCR analyses [53]. In 2011, a 7-plex was developed [46] while an 8-plex and 10-plex were reported elsewhere [27].…”

Section: X-chromosomal Strs and Ministrs: Identity Testing And Beyondmentioning

confidence: 99%

Scope of X-Chromosomal MiniSTRs: Current Developments

Israr¹

2014

J Forensic

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The experience of using autosomal short-length amplicon STRs or miniSTRs in profiling of degraded DNA and mass disaster victims is extended into the realm of X-chromosomal (ChrX) STR miniaturization. About half of the total X-STRs are now short-length amplicons and the focus is shifting to using the mini versions of all of them. Joint multiplexing of these loci can be used for solving complex paternity cases and association of mass disaster victims with their families. This technology may herald a new dimension for research in population genetics and evolution. We present an overview of the progress made thus far and the future scope and prospects for X-miniSTRs.

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“…STRs are advantageous as genetic markers because they are highly polymorphic and have a wide range of applications in forensic science and casework, including individual identification and the study of relationship between populations. Forensic STR analysis can be performed with biological evidence, such as blood cells, buccal cells and bones [1,2].…”

Section: Introductionmentioning

confidence: 99%

Polymorphism of the Microsatellite DXS8377 in a Population of Northern Thai Males

Leewattanapasuk¹,

Kunjae²,

Sangthong³

et al. 2016

J Forensic Res

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Objective: This research investigated the polymorphism of the X-chromosomal short tandem repeats (X-STR) DXS8377 in Northern Thai males.Methods: Genomic DNA was extracted from blood or buccal cells of 200 unrelated healthy individuals using Nucleospin® Blood or Nucleospin® Tissue (Macherey-Nagel), respectively. The DXS8377 locus was amplified by polymerase chain reaction (PCR) and the PCR product was analyzed by agarose gel electrophoresis. The allelic ladder was constructed and the size of all PCR products was analyzed by polyacrylamide gel electrophoresis using allelic ladder as a marker. The nucleotide sequences of the different-sized PCR products were analyzed to identify the DXS8377 alleles. Lastly, the Polymorphic information content (PIC) and Power of discrimination (PD) of DXS8377 DNA marker were calculated and the analysis of population relationships was performed using chi-square test of homogeneity.Results: Eleven DXS8377 alleles were observed in the study population, the allele 49 was the most frequent with the allele frequency of 0.4400. According to previous studies, allele 49 was found in all of the populations studied and it was common among various populations. The polymorphism and the probability of DXS8377 to discriminate two unrelated individuals were moderate with a PIC and PD of 0.6973 and 0.7305, respectively. Most interestingly, the DXS8377 DNA marker was able to differentiate Mongoloid from Caucasoid and Negroid and it was also able to differentiate Northern Thai males from other Asian populations, including Korean, Japanese and Chinese.

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Applications of DNA Typing

Cited by 62 publications

References 89 publications

Probabilistic characterisation of baseline noise in STR profiles

Probabilistic characterisation of baseline noise in STR profiles

Scope of X-Chromosomal MiniSTRs: Current Developments

Polymorphism of the Microsatellite DXS8377 in a Population of Northern Thai Males

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