Applications of digital PCR in COVID‐19 pandemic

Tan, Chianru; Fan, Dongdong; Wang, Nan; Wang, Fang; Wang, Bo; Zhu, Lingxiang; Guo, Yong

doi:10.1002/viw.20200082

Cited by 41 publications

(55 citation statements)

References 30 publications

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“…The dPCR method can be classified into three types based on liquid separation: droplet-based (ddPCR), chip-based (cdPCR), and microfluidic digital PCR (mdPCR). The primary difference between these three types of digital PCR is the design of the sample partitioning system in the detection platform: ddPCR combines several millions partitioning of the PCR test into individual droplets in a water-in-oil emulsion [26,28], whereas cdPCR uses an active partitioning approach. It has two chip halves with two arrays of microwells.…”

Section: Digital Rt-pcrmentioning

confidence: 99%

“…It has two chip halves with two arrays of microwells. The chambers are aligned so that the opposite halves form continuous channels [28,29]. In mdPCR, microfluidic chambers are used to split the samples.…”

Section: Digital Rt-pcrmentioning

confidence: 99%

“…The integration of electrochemistry with RT-PCR aims to provide a rapid, miniatur- dPCR can be used for the quantification of a low viral load, monitoring of the virus in the environment, evaluation of anti-SARS-CoV-2 drugs [28], and the detection of viral mutations [31]. Many types of clinical samples can be used for COVID-19 testing using dPCR, including blood, urine, sputum, stool, nasal swabs, and throat swabs.…”

Section: Electrochemical Pcr: Unexplored Potentialmentioning

confidence: 99%

“…Figure 2. Schematic depicting workflow of a ddPCR system: (A) preparation for amplification, (B) generation of water-in-oil droplets using a microfluidic flow system, (C) collection of the droplets in PCR tubes, (D) PCR amplification, (E) analysis of fluorescence in the droplets after amplification, and (F) fitting to Poisson distribution to determine the absolute copy numbers of the target molecules (Reprinted from Ref [28]…”

mentioning

confidence: 99%

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Point-of-Care PCR Assays for COVID-19 Detection

et al. 2021

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Molecular diagnostics has been the front runner in the world’s response to the COVID-19 pandemic. Particularly, reverse transcriptase-polymerase chain reaction (RT-PCR) and the quantitative variant (qRT-PCR) have been the gold standard for COVID-19 diagnosis. However, faster antigen tests and other point-of-care (POC) devices have also played a significant role in containing the spread of SARS-CoV-2 by facilitating mass screening and delivering results in less time. Thus, despite the higher sensitivity and specificity of the RT-PCR assays, the impact of POC tests cannot be ignored. As a consequence, there has been an increased interest in the development of miniaturized, high-throughput, and automated PCR systems, many of which can be used at point-of-care. This review summarizes the recent advances in the development of miniaturized PCR systems with an emphasis on COVID-19 detection. The distinct features of digital PCR and electrochemical PCR are detailed along with the challenges. The potential of CRISPR/Cas technology for POC diagnostics is also highlighted. Commercial RT–PCR POC systems approved by various agencies for COVID-19 detection are discussed.

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Section: Digital Rt-pcrmentioning

confidence: 99%

Section: Digital Rt-pcrmentioning

confidence: 99%

Section: Electrochemical Pcr: Unexplored Potentialmentioning

confidence: 99%

mentioning

confidence: 99%

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Point-of-Care PCR Assays for COVID-19 Detection

et al. 2021

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“…7 . (A) Schematics of qPCR and droplet digital PCR (image modified from ( Tan et al, 2021 )), (B) workflow of the isothermal amplification process (image taken from ( Khan et al, 2020 ) with permission, © 2020 American Chemical Society), and (C) paper-based isothermal amplification technique (RT-LAMP) integrated with VIVAS for collection and detection of airborne influenza A H1N1 viruses (image taken from ( Jiang et al, 2021 )). …”

Section: Virus Measurementsmentioning

confidence: 99%

Recent advancements in the measurement of pathogenic airborne viruses

Bhardwaj

Hong

Jang

et al. 2021

Journal of Hazardous Materials

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Air-transmissible pathogenic viruses, such as influenza viruses and coronaviruses, are some of the most fatal strains and spread rapidly by air, necessitating quick and stable measurements from sample air volumes to prevent further spread of diseases and to take appropriate steps rapidly. Measurements of airborne viruses generally require their collection into liquids or onto solid surfaces, with subsequent hydrosolization and then analysis using the growth method, nucleic-acid-based techniques, or immunoassays. Measurements can also be performed in real time without sampling, where species-specific determination is generally disabled. In this review, we introduce some recent advancements in the measurement of pathogenic airborne viruses. Air sampling and measurement technologies for viral aerosols are reviewed, with special focus on the effects of air sampling on damage to the sampled viruses and their measurements. Measurement of pathogenic airborne viruses is an interdisciplinary research area that requires understanding of both aerosol technology and biotechnology to effectively address the issues. Hence, this review is expected to provide some useful guidelines regarding appropriate air sampling and virus detection methods for particular applications.

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Thermally Engineered Ferric Yolk‐Shells for Vaginal Secretion Based Cervical Cancer Screening

Wu,

Wei,

Shi

et al. 2024

Adv Funct Materials

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Engineering of materials is crucial for the advanced detection of bio‐molecules, particularly in laser desorption/ionization mass spectrometry (LDI‐MS). However, effective screening with enhanced LDI performance remains challenging due to the lack of engineered substrate materials. Herein, thermally engineered ferric yolk‐shells are designed for enhanced metabolic screening of cervical cancer (CC) using vaginal secretion. A facile thermal engineering strategy is developed to prepare amorphous ferric yolk‐shells with integrated properties. Notably, the amorphous mesoporous yolk‐shells offer signal amplification up to orders of magnitude in LDI‐MS for standard metabolites and diverse bio‐samples. High‐performance CC screening (including identification of human papillomavirus (HPV) infection, high‐grade squamous intraepithelial lesion (HSIL), and CC) is achieved, with the areas under the curve of 0.842–0.963. Finally, a metabolic panel for CC screening, covering the stages of HPV, HSIL, and CC, is established. This work elucidates the LDI‐MS mechanism for enhanced metabolic detection and highlights potential applications in cancer screening.

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Applications of digital PCR in COVID‐19 pandemic

Cited by 41 publications

References 30 publications

Point-of-Care PCR Assays for COVID-19 Detection

Point-of-Care PCR Assays for COVID-19 Detection

Recent advancements in the measurement of pathogenic airborne viruses

Thermally Engineered Ferric Yolk‐Shells for Vaginal Secretion Based Cervical Cancer Screening

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