Application of UHPLC-Q-TOF MS based untargeted metabolomics reveals variation and correlation amongst different tissues of Eucommia ulmoides Oliver

Chen, Jiangyan; Wang, Weitao; Kong, Jiaqi; Yue, Yadong; Dong, Yiyang; Zhang, Jiaheng; Liu, Li

doi:10.1016/j.microc.2021.106919

Cited by 25 publications

(6 citation statements)

References 32 publications

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“…Correlation analysis is a powerful tool for characterising the metabolic proximities among metabolites having significant differences 29 . Hence, a total of 159 differential metabolites in O. gracilis cultures were analysed by generating a correlation matrix using Pearson's correlation in order to find the mutual regulatory relationship among metabolites in the process of O. gracilis in different culture conditions.…”

Section: Resultsmentioning

confidence: 99%

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Wang

Yang

Tong

et al. 2023

Phytochemical Analysis

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IntroductionOphiocordyceps gracilis, a type of edible and medicinal fungus, exhibits multiple health‐promoting effects. Due to the scarcity of natural O. gracilis, artificial cultures have been developed as its substitutes. However, lacking comprehension of the metabolite composition of cultures limits its utilisation.ObjectiveThis research aimed to evaluate the nutritional and medicinal value of four cultures of O. gracilis by analysing their metabolite composition. In addition, metabolic pathways in mycelia and fruiting bodies were analysed to explore fruiting body formation mechanism at metabolic level.MethodThe mycelia, fermentation broth, spore powder and fruiting bodies of O. gracilis were cultivated in this study. Their metabolite composition was compared using an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry (LC–MS/MS).ResultsPrincipal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS‐DA) showed that the four cultures have noticeable differences in metabolite composition. A total of 612 metabolites were identified, among which 159 metabolites showed significant differences, and these differential metabolites were classified into 13 categories. The metabolites in the fruiting bodies were the most abundant compared with other cultures. However, each culture had its own advantages and significantly accumulates some active metabolites respectively. Pearson's correlation analysed the mutual relationship among metabolites. In addition, seven metabolic pathways were closely related to fruiting body formation, such as “Biosynthesis of plant secondary metabolites”, “amino acids metabolism”, “tricarboxylic acid (TCA) cycle”.ConclusionThis study offered a reference to mycelia, fermentation broth, spore powder and fruiting bodies of O. gracilis as health‐promoting functional foods and medicine.

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Section: Resultsmentioning

confidence: 99%

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Wang

Yang

Tong

et al. 2023

Phytochemical Analysis

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“…Related studies had been conducted in diverse tea populations ( Yu et al., 2020 ). Chemical components in different tissues of E.ulmoides including leaves, seeds and barks had been determined by ultra-high-performance liquid chromatography-tandem time-of-flight mass spectrometer (UHPLC-QTOF/MS) untargeted metabolomics, and finally 2,373 metabolites were identified in total ( Chen et al., 2022 ). Besides, the dynamic metabolic models for leaf growth and development of E.ulmoides were constructed by integrated uses of widely targeted metabolomics and transcriptomics ( Li et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Analysis of chemotypes and their markers in leaves of core collections of Eucommia ulmoides using metabolomics

Meng

Du²,

Du³

et al. 2023

Front. Plant Sci.

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The leaves of Eucommia ulmoides contain various active compunds and nutritional components, and have successively been included as raw materials in the Chinese Pharmacopoeia, the Health Food Raw Material Catalogue, and the Feed Raw Material Catalogue. Core collections of E. ulmoides had been constructed from the conserved germplasm resources basing on molecular markers and morphological traits, however, the metabolite diversity and variation in this core population were little understood. Metabolite profiles of E. ulmoides leaves of 193 core collections were comprehensively characterized by GC-MS and LC-MS/MS based non-targeted metabolomics in present study. Totally 1,100 metabolites were identified and that belonged to 18 categories, and contained 120 active ingredients for traditional Chinese medicine (TCM) and 85 disease-resistant metabolites. Four leaf chemotypes of the core collections were established by integrated uses of unsupervised self-organizing map (SOM), supervised orthogonal partial least squares discriminant analysis (OPLS-DA) and random forest (RF) statistical methods, 30, 23, 43, and 23 chemomarkers were screened corresponding to the four chemotypes, respectively. The morphological markers for the chemotypes were obtained by weighted gene co-expression network analysis (WGCNA) between the chenomarkers and the morphological traits, with leaf length (LL), chlorophyll reference value (CRV), leaf dentate height (LDH), and leaf thickness (LT) corresponding to chemotypes I, II, III, and IV, respectively. Contents of quercetin-3-O-pentosidine, isoquercitrin were closely correlated to LL, leaf area (LA), and leaf perimeter (LP), suggesting the quercetin derivatives might influence the growth and development of E. ulmoides leaf shape.

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“…Partial least squares discriminant analysis is a supervised model analysis method based on a latent variable regression approach of the variance between predictor and response variables that has been shown to be effective in handling datasets with multicollinearity predictor variables (Chen et al, 2022). The samples are also clearly divided into four groups in the PLS-DA score plot, indicating that the method is accurate and reliable (Figure 4c,d).…”

Section: Partial Least Squares Discriminant Analysismentioning

confidence: 99%

Chemical characterization, multivariate analysis and comparison of biological activities of different parts of Fraxinus mandshurica

Guo,

Liu,

Guo

et al. 2024

Biomedical Chromatography

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Fraxinus mandshurica (Oleaceae) is used as a traditional medicinal plant for the treatment of red eyes, menstrual disorders, excessive leucorrhea, chronic bronchitis and psoriasis. To perform chemical characterization of the secondary metabolites of F. mandshurica roots, bark, stems and leaves, 32 samples were collected from eight provinces in this study. A total of 64 chemical components were detected from four different parts of F. mandshurica by ultra‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry. Meanwhile, a total of nine secoiridoids were obtained by natural product chemical extraction, isolation and identification methods. Quantitative analysis by high‐performance liquid chromatography–diode array detection–mass spectrometry showed the highest total content of secoiridoids in the bark, which is also consistent with the traditional medicinal parts. The results of methodological validation showed that the correlation coefficient (R2) values were all >0.9993, indicating a good linear range of the standard curve, while the relative standard deviations of precision, reproducibility and stability were <3%, and the spiked recoveries ranged from 98.22 to 102.27%, indicating that the experimental method was reliable and stable. In addition, fingerprinting and a heatmap were established to demonstrate the content trends of F. mandshurica more visually from different origins. Multivariate analysis, including principal component analysis and partial least squares discriminant analysis, was performed to determine the chemical characteristics of different parts of F. mandshurica, and six characteristic secoiridoids that could be used to distinguish different origins were screened. Finally, the inhibition of tyrosinase, α‐glucosidase, acetylcholinesterase and pancreatic lipase activities by the nine characteristic compounds and extracts from different parts were investigated, and the results showed that they all exhibited different degrees of enzyme activity inhibition and thus have potential applications in whitening and blemish removal, hypoglycemia, anti‐Alzheimer's disease and anti‐obesity as a new source of natural enzyme activity inhibitors. This study establishes an identification and evaluation method applicable to phytochemistry of different origins, which is a guideline for quality control, origin evaluation and clinical application of traditional medicinal plants. This is also an unprecedented study on the identification of the chemical composition of different parts of F. mandshurica, characteristic compounds and the inhibition of enzyme activity of extracts from different parts.

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Application of UHPLC-Q-TOF MS based untargeted metabolomics reveals variation and correlation amongst different tissues of Eucommia ulmoides Oliver

Cited by 25 publications

References 32 publications

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Comparative metabolic profiling of mycelia, fermentation broth, spore powder and fruiting bodies of Ophiocordyceps gracilis by LC–MS/MS

Analysis of chemotypes and their markers in leaves of core collections of Eucommia ulmoides using metabolomics

Chemical characterization, multivariate analysis and comparison of biological activities of different parts of Fraxinus mandshurica

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