“…We used a multiple allergen simultaneous test assay (Hitachi Chemical Diagnostics, Mountain View, CA, USA) containing 20 kinds of food antigens, such as wheat, milk, egg, buckwheat, shrimp, crab, soybean, and more. This assay can measure several allergen-specific IgE simultaneously in each serum, 16 and the sensitivity and specificity were 85% and 82%, respectively. 17 The results of this assay are represented as lumi-count (LC) and class (class 0 = 0-1.39, class 1 = 1.40-2.77, class 2 = 2.78-13.4, class 3 = 13.5-58.0, class 4 = 58.1-119, class 5 = 120-150, and class 6 = 160 or above).…”