Application of the MAST Immunodiagnostic System to the determination of allergen-specific IgE.

Miller, Steven P.; Marinkovich, Vincent A.; Riege, D H; Sell, W J; Baker, David L.; Eldredge, Noreen T.; Dyminski, John W.; Hale, Ralph W.; Peisach, Jack; Burd, John F.

doi:10.1093/clinchem/30.9.1467

Cited by 39 publications

(16 citation statements)

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“… CD4/CD8 ratio 1.77 (1.04 - 3.11) 1.92 (1.23 - 4.01) n.s. Significance was calculated using Wilcoxon rank-sum (Mann–Whitney) test *Lumicount by multiple allergen simultaneous test (MAST)-26 chemiluminescent assay systems [ 7 , 12 ] …”

Section: Resultsmentioning

confidence: 99%

“…*Lumicount by multiple allergen simultaneous test (MAST)-26 chemiluminescent assay systems [ 7 , 12 ]…”

Section: Resultsmentioning

confidence: 99%

“…Total IgE, total IgG, cortisol, IFN-gamma, IL-1beta, IL-2, IL-5, IL-4, IL-6, and the CD4/CD8 ratio in serum were measured by SRL, Inc., Tokyo, Japan (Additional file 1 ). Specific serum IgE antibody was measured with the multiple allergen simultaneous test (MAST)-26 chemiluminescent assay systems (Hitachi Chemical Co., Tokyo, Japan) [ 7 ].…”

Section: Methodsmentioning

confidence: 99%

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Comparison of IgG against plastic resin in workers with and without chemical dermatitis

2015

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BackgroundThere are many chemical sensitizers which cause allergy in the surrounding environment. However, the identification of substances causing allergy is difficult. We developed a new method to detect IgG which reacts against many kinds of chemical-human serum albumin (HSA) adducts at the same time. In this study, the diagnostic significance of the IgG was studied among workers of a company where a mass outbreak of chemical dermatitis had occurred after changing a plastic resin to a new one.MethodsEleven workers who handled the new plastic resin and suffered from dermatitis (case) and 9 workers who also handled the same resin in the same company but were free from dermatitis (control) were the subjects. Immunological dot blotting was carried out to detect serum IgG using originally prepared diagnostic antigens, comprising a mixture of HSA and the plastic resin or its components under various conditions.ResultsIgG against the plastic resin in use was detected in all workers who suffered from dermatitis. The prevalence of the IgG against the plastic resin was significantly higher in workers with than in those without dermatitis. On the other hand, IgG against its components (bisphenol A diglycidyl ether, m-xylylenediamine and butyl 2,3-epoxypropyl ether) was detected in a few workers with dermatitis.DiscussionThis suggests that IgG against chemical-HSA adduct reflects not only exposure but also causative chemicals of dermatitis. Our method to use a material itself as a hapten is practical and useful in the occupational field.ConclusionIt is suggested that IgG against chemicals is a useful marker of chemicals inducing dermatitis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12889-015-2302-4) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

“…*Lumicount by multiple allergen simultaneous test (MAST)-26 chemiluminescent assay systems [ 7 , 12 ]…”

Section: Resultsmentioning

confidence: 99%

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Comparison of IgG against plastic resin in workers with and without chemical dermatitis

2015

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“…We used a multiple allergen simultaneous test assay (Hitachi Chemical Diagnostics, Mountain View, CA, USA) containing 20 kinds of food antigens, such as wheat, milk, egg, buckwheat, shrimp, crab, soybean, and more. This assay can measure several allergen-specific IgE simultaneously in each serum, 16 and the sensitivity and specificity were 85% and 82%, respectively. 17 The results of this assay are represented as lumi-count (LC) and class (class 0 = 0-1.39, class 1 = 1.40-2.77, class 2 = 2.78-13.4, class 3 = 13.5-58.0, class 4 = 58.1-119, class 5 = 120-150, and class 6 = 160 or above).…”

Section: Serum Cytokines and Serological Food Hypersensitivitymentioning

confidence: 99%

Cytokine Profile and Immunoglobulin E-mediated Serological Food Hypersensitivity in Patients With Irritable Bowel Syndrome With Diarrhea

Katsumata

Ishii

Lee

et al. 2018

J Neurogastroenterol Motil

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Background/AimsFood interaction, including food hypersensitivity, plays a key role in the pathogenesis of irritable bowel syndrome with diarrhea (IBS-D). Since only a few studies have been reported about the relationship between food hypersensitivity and IBS-D, we elucidate the prevalence of serological food hypersensitivity in patients with IBS-D and the characteristics of gastrointestinal symptoms and serum cytokine profiles in patients with IBS-D and serological food hypersensitivity.MethodsImmunoglobulin E (Ig E)-mediated serological food hypersensitivity and serum cytokine levels were evaluated using the multiple allergen simultaneous test evaluating food allergen-specific serum IgE and Luminex Milliplex Panel containing multiple fluorescence-labeled beads. Class 2 or above was considered as IgE-mediated food hypersensitivity positive. The gastrointestinal symptom rating scale was used to evaluate symptoms.ResultsWe enrolled 92 subjects, including 60 with IBS-D and 32 healthy controls. The percentages of patients with IgE-mediated serological food hypersensitivity were not significantly different between the groups (controls = 28.1% and IBS-D = 33.3%). Serum IL-1β, IL-6, IL-8, macrophage inflammatory protein-1alpha, and TNF-α levels were higher in patients with IBS-D than in controls. Serum concentration of TNF-α (43.4 vs 21.8 pg/mL, P = 0.009) was higher in patients with IBS-D without IgE-mediated serological food hypersensitivity than those with food hypersensitivity.ConclusionsOne-third of Japanese patients with IBS-D showed IgE-mediated serological food hypersensitivity. The serum cytokine profile differed and was characterized by lower inflammatory cytokine levels in IBS-D with IgE-mediated serological food hypersensitivity. Serological test regarding IgE-mediated food hypersensitivity can detect a certain cluster of IBS-D.

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“…Specific IgE antibodies to a wide panel of food allergens were measured, using Multiple Antigen Simultaneous Test (MAST) 26 system in Patients 1 and 2, and by MAST 33 in Patients 3–5 (Miller et al , ). Classes of 1 or more and 2 or more were defined as positive respectively.…”

Section: Characteristics Of Five Patients Who Developed Fa After Cbtmentioning

confidence: 99%

Food allergy after cord blood transplantation in children

et al. 2012

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Application of the MAST Immunodiagnostic System to the determination of allergen-specific IgE.

Cited by 39 publications

References 0 publications

Comparison of IgG against plastic resin in workers with and without chemical dermatitis

Comparison of IgG against plastic resin in workers with and without chemical dermatitis

Cytokine Profile and Immunoglobulin E-mediated Serological Food Hypersensitivity in Patients With Irritable Bowel Syndrome With Diarrhea

Food allergy after cord blood transplantation in children

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