Comparison of IgG against plastic resin in workers with and without chemical dermatitis

Abstract

BackgroundThere are many chemical sensitizers which cause allergy in the surrounding environment. However, the identification of substances causing allergy is difficult. We developed a new method to detect IgG which reacts against many kinds of chemical-human serum albumin (HSA) adducts at the same time. In this study, the diagnostic significance of the IgG was studied among workers of a company where a mass outbreak of chemical dermatitis had occurred after changing a plastic resin to a new one.MethodsEleven workers who handled the new plastic resin and suffered from dermatitis (case) and 9 workers who also handled the same resin in the same company but were free from dermatitis (control) were the subjects. Immunological dot blotting was carried out to detect serum IgG using originally prepared diagnostic antigens, comprising a mixture of HSA and the plastic resin or its components under various conditions.ResultsIgG against the plastic resin in use was detected in all workers who suffered from dermatitis. The prevalence of the IgG against the plastic resin was significantly higher in workers with than in those without dermatitis. On the other hand, IgG against its components (bisphenol A diglycidyl ether, m-xylylenediamine and butyl 2,3-epoxypropyl ether) was detected in a few workers with dermatitis.DiscussionThis suggests that IgG against chemical-HSA adduct reflects not only exposure but also causative chemicals of dermatitis. Our method to use a material itself as a hapten is practical and useful in the occupational field.ConclusionIt is suggested that IgG against chemicals is a useful marker of chemicals inducing dermatitis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12889-015-2302-4) contains supplementary material, which is available to authorized users.

A Simple Method for Detection of Multiple Chemical-Specific IgGs in Serum Based on Dot Blotting

“…We have previously reported that the antibody for Product-X, a mixture of epoxy resin compounds, in human serum can be detected by dot blot assay using X-HSA adducts as the antigen. Moreover, we also showed that the levels of Product-X-specific IgG in human serum are strongly correlated with the onset of dermatitis [9]. In this study, we attempted to simultaneously detect several specific antibodies against chemicals, such as TDI, PA, and FA in human serum from four workers handling plastic resin who had a history of allergy and from five unexposed workers.…”

“…Dot blots were performed as previously reported [9]. The antigens were blotted to nitrocellulose membranes (Amersham, GE Healthcare), which were then blocked with blocking buffer (Nacalai Tesque).…”

Association between bisphenol A diglycidyl ether-specific IgG in serum and food sensitization in young children

“…Importantly, however, it has also been reported to have estrogenic activity, albeit weaker than that of BPA [3]. In our previous study, BADGE-specific IgG was identified in sera obtained from workers with contact dermatitis who had used this material in their working space [4]. This result suggested that serum BADGE-specific IgG could reflect exposure to BADGE and that exposure is related to allergic diseases.…”

“…Recent studies have reported that exposure to endocrine-disrupting compounds (EDCs) including BPA and BADGE might increase the risk of allergic diseases such as respiratory allergies, contact dermatitis, food sensitization, and food tolerance [4–7]. BPA was suggested to induce a T helper 2 (Th2)-dominated immune response through its estrogenic activity [8].…”

Determination of chemical-specific IgGs in serum by an enzyme-linked immunosorbent assay with partial peptides of human serum albumin

“…An ELISA using formaldehyde (FA) and phthalic anhydride (PA)-HSA adducts as a substrate was also reported (Dykewicz et al, 1991;Nielsen et al, 1988). We previously reported a dot blotting-based method for the detection of chemical-specific IgGs, including TDI, FA and PA (Tsuji et al, 2016;Kawamoto et al, 2015).…”