Determination of chemical-specific IgGs in serum by an enzyme-linked immunosorbent assay with partial peptides of human serum albumin

Abstract

-Many different types of chemicals are used in industry, and occupational allergies are becoming a serious problem in the field of industrial hygiene. In this study, we employed a novel enzymelinked immunosorbent assay (ELISA) with partial peptides of human serum albumin (HSA) to quantify chemical-specific immunoglobulin G (IgG) in serum for evaluating exposure to chemicals. When HSA partial peptides containing lysine residues were mixed with formaldehyde (FA) or phthalic anhydride (PA), almost all lysine residues were lost. Mass spectrometry revealed that PA and FA formed imine and tertiary amine, respectively, with lysine residues in the peptides. Thus, we used FA-or PA-peptide adducts as an artificial antigen to detect FA-and PA-specific IgGs in serum. The concentrations of FA-and PAspecific IgGs in workers at plants utilizing plastic resins were significantly higher than those in general subjects. This method can estimate exposure levels to chemicals and thus be expected to contribute to the diagnosis of allergies in workers and to the prevention of health hazards due to harmful chemicals.

Association between bisphenol A diglycidyl ether-specific IgG in serum and food sensitization in young children

“…The subjects with both BADGE-specific IgG and increased wheat-specific IgE levels presented with increased IL - 6 mRNA expression compared to the other groups. Serum BADGE-specific IgGs probably reflect BADGE exposure even though the association with dose–response is unclear [4, 28]. In our previous study [4], 90% (9 of 10) of workers who had used epoxy resin and developed contact dermatitis had BADGE-specific IgG.…”