Application of the Biological Conjugate between Antibody and Colloid Au Nanoparticles as Analyte to Inductively Coupled Plasma Mass Spectrometry

Abstract

This paper describes the study of atomization of nanoparticles by inductively coupled plasma mass spectrometry (ICPMS) and developes a novel nonisotopic immunoassay by coupling sandwich-type immunoreaction to ICPMS. The goat-anti-rabbit immunoglobulin G (IgG) labeled with colloidal gold nanoparticles served as an analyte in ICPMS for the indirect measurement of rabbit-anti-human IgG. Matrix effect studies showed the gold signal was not sensitive to the organic matrix. A relatively good correlation (r2 = 0.9528) between the proposed method and enzyme-linked immunosorbent assay has been obtained. The method may have significant potential as an important ICPMS-based nonisotopic immnoassay method for the simultaneous determination of biologic analytes of interest by labeling different kinds of inorganic nanoparticles.

A sensitive immunosensor using colloidal gold as electrochemical label

“…Some instruments involved are expensive and require skillful personnel to operate. Many other methods, such as inductively coupled plasma mass spectrometry [9], chemiluminescence [10], electrochemical stripping technique [11,12] have also been reported to measure the amount of colloidal gold in immunoassay or DNA assay. All of these methods require dissolving the gold nanoparticles using bromine or other oxidants * Corresponding author.…”

Exploration of nano-surface chemistry for spectral analysis

“…Moreover, the specific activities of biomolecules can be retained when coupling biomolecules to colloidal Au nanoparticles. Au NPs have been applied in ICPMS-based immunoassay since the pioneer works reported by Zhang et al [91] and later by Tanner goupe [92]. Those methods could achieve the detection limits of enzyme-linked immunosorbent assay (ELISA), and their sensitivity can be further enhanced by single particle mode detection [93,94].…”

Inorganic mass spectrometry as a tool for characterisation at the nanoscale

“…Within the field of atomic spectrometry, the concept of elemental labelling with elemental nanostructures was pioneered some years ago by Zhang et al [117] and by Baranov et al [116]. However, this area of research is still of great novelty, with a high potential for future applications and continual development.…”

“…However, this area of research is still of great novelty, with a high potential for future applications and continual development. In pioneer work, Zhang et al [117] successfully demonstrated the determination of rabbit-anti-human immunoglobulin G (IgG) by ICP-MS using antibodies labelled with colloidal Au NPs. In this work, a sandwich-type immunoreaction was investigated as the format for the measurement of the IgG.…”