A sensitive immunosensor using colloidal gold as electrochemical label

Chen, Zhao-Peng; Peng, Zhaoliang; Zhang, Peng; Jin, Xuefang; Jiang, Jian‐Hui; Zhang, Xiaobing; Shen, Guo‐Li; Yu, Ru‐Qin

doi:10.1016/j.talanta.2007.02.020

Cited by 48 publications

(21 citation statements)

References 15 publications

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“…54 To optimize the condition of the conjugation, we adjusted the pH value of GNP solution and the amount of conjugating antibody and found that the best condition of pH value was 8 and the amount of conjugating antibody was ~20 µg (i.e., 6.8 mg/mL). Accordingly, 4 µL of 0.2 M potassium carbonate (to make sure the pH value is 8) and 3 µL conjugating antibody ( Figure S2) were successively added into the as-prepared 1 mL GNP solution for 10 min incubation at RT.…”

mentioning

confidence: 99%

Improved LFIAs for highly sensitive detection of BNP at point-of-care

Gong¹,

Hu²,

Choi³

et al. 2017

IJN

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Heart failure (HF) has become a major cause of morbidity and mortality with a significant global economic burden. Although well-established clinical tests could provide early diagnosis, access to these tests is limited in developing countries, where a relatively higher incidence of HF is present. This has prompted an urgent need for developing a cost-effective, rapid and robust diagnostic tool for point-of-care (POC) detection of HF. Lateral flow immunoassay (LFIA) has found widespread applications in POC diagnostics. However, the low sensitivity of LFIA limits its ability to detect important HF biomarkers (e.g., brain natriuretic peptide [BNP]) that are normally present in low concentration in blood. To address this issue, we developed an improved LFIA by optimizing the gold nanoparticle (GNP)–antibody conjugate conditions (e.g., the conjugate pH and the amount of added antibody), the diameter of GNP and the concentration of antibody embedded on the test line and modifying the structure of test strip. Through these improvements, the proposed test strip enabled the detection of BNP down to 0.1 ng/mL within 10–15 min, presenting ~15-fold sensitivity enhancement over conventional lateral flow assay. We also successfully applied our LFIA in the analysis of BNP in human serum samples, highlighting its potential use for clinical assessment of HF. The developed LFIA for BNP could rapidly rule out HF with the naked eye, offering tremendous potential for POC test and personalized medicine.

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confidence: 99%

Improved LFIAs for highly sensitive detection of BNP at point-of-care

Gong¹,

Hu²,

Choi³

et al. 2017

IJN

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“…The detection limit for the immunosensor was 4.0 ng mL −1 . [23] These are just a few examples of systems in which charge or other physical phenomena lead to antibody conjugates, and many research groups have carried out similar noncovalent modes of antibody-nanoparticle conjugations. The bioconjugation protocols have been mainly based on a combination of electrostatic and hydrophobic interactions among antibody and GNP surface, which have wide applications in the field of biomedical imaging.…”

Section: Antibody-conjugated Nanoparticle Using Physical Adsorptionmentioning

confidence: 99%

Recent Advances in the Generation of Antibody–Nanomaterial Conjugates

Sivaram

Wardiana

Howard

et al. 2017

Adv Healthcare Materials

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Targeted nanomedicines have significantly changed the way new therapeutics are designed to treat disease. Central to successful therapeutics is the ability to control the dynamics of protein–nanomaterial interactions to enhance the therapeutic effect of the nanomedicine. The aim of this review is to illustrate the diversity and versatility of the conjugation approaches involved in the synthesis of antibody–nanoparticle conjugates, and highlight significant new advances in the field of bioconjugation. Such nanomedicines have found utility as both advanced therapeutic agents, as well as more complex imaging contrast agents that can provide both anatomical and functional information of diseased tissue. While such conjugates show significant promise as next generation targeted nanomedicines, it is recognized that there are in fact no clinically approved targeted therapeutics on the market. This fact is reflected upon within this review, and attempts are made to draw some reasoning from the complexities associated with the bioconjugation chemistry approaches that are typically utilized. Present trends, as well as future directions of next generation targeted nanomedicines are also discussed.

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“…The UV-vis spectrometer showed an absorption peak at 525 nm. The antibody-colloid gold conjugate was prepared according to the procedure described by Chen et al [5]. The antibody-colloidal gold conjugate was prepared by adding 100 µL of anti-tetracycline antibody (1.0 mg·mL -1 ) to 1.0 mL of pH-adjusted colloidal gold solution (pH 9.0), followed by slow shaking for one hour at room temperature.…”

Section: Preparation Of Conjugated Gold Nano-particles With Antibody mentioning

confidence: 99%

Development of Immunosensor for Early Detection of Aquatic Harmful Algal Blooms (HABs)

Salam¹,

Razali²,

Rahman³

et al. 2017

JMSE-B

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Blooms of toxic and harmful microalgae, known as "red tides", represent a significant and tremendous threat to human health and fisheries resources throughout the Southeast Asia region and the world. A harmful microalga which produces secondary metabolite known as saxitoxin (STX) is a neurotoxin produced by a variety of genera of dinoflagellates. The ingestion of this toxin through contaminated shellfish will inhibit the neuron depolarizations and action potentials which result in respiratory failure in human and known as PSP (paralytic shellfish poisoning). PSP takes effect as soon as five minutes depending on the species and the concentration consumed. Currently, the identification of dinoflagellates cells is done by taxonomy, which is based on a broad expertise of specially trained staff, expensive equipment like electron microscopes and is very time consuming. In many countries, for the toxin monitoring, they use mouse bio-assay which is bulky, expensive, loss of animal life and high variability and higher limit of detection (40 µg per 100 g meat) and HPLC methods required many pre-treatment steps for the analysis. Therefore, in order to overcome this problem, development of early detection of harmful algae bloom and bio-toxin in aquatic ecosystem is needed. The detection is based on screen-printed carbon working electrode with onboard carbon counter and silver-silver chloride pseudo-reference electrode. A direct ELISA (enzyme-linked immunosorbent assay) format was developed and optimized on the surface of a carbon screen-printed working electrode by immobilizing the capture antibody using electro-deposition of gold nanoparticles conjugated with polyclonal anti-toxin antibodies. The detection reagent, toxin-HRP (horseradish peroxidase) conjugate was used as an enzyme label. Electrochemical detection was then carried out using 3,3',5,5'-tetramethylbenzidine dihydrochloride (TMB)/H 2 O 2 as the enzyme mediator/substrate system and conducted using chronoamperometry at 100 mV vs. onboard screen-printed Ag-AgCl pseudo-reference electrode. The minimum detection limits for harmful micro algae about 1 cell per mL. The immunosensor is very selective which is able to detect Alexandrium minitum with 100% selectivity and minimal cross reaction with others nontoxic algae (< 2%).

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A sensitive immunosensor using colloidal gold as electrochemical label

Cited by 48 publications

References 15 publications

Improved LFIAs for highly sensitive detection of BNP at point-of-care

Improved LFIAs for highly sensitive detection of BNP at point-of-care

Recent Advances in the Generation of Antibody–Nanomaterial Conjugates

Development of Immunosensor for Early Detection of Aquatic Harmful Algal Blooms (HABs)

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